A5027053971- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Platelet Disorders and Treatments
- Influenza Virus Research Studies
- Carbohydrate Chemistry and Synthesis
- SARS-CoV-2 and COVID-19 Research
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Phytoplasmas and Hemiptera pathogens
- Bone and Dental Protein Studies
- Plant Pathogenic Bacteria Studies
- Genetics and Neurodevelopmental Disorders
- Thyroid Cancer Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Ubiquitin and proteasome pathways
- Pancreatic function and diabetes
- Viral Infections and Vectors
- Phytochemical compounds biological activities
- Cocoa and Sweet Potato Agronomy
- Natural product bioactivities and synthesis
- Fibroblast Growth Factor Research
- Skin and Cellular Biology Research
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Thyroid and Parathyroid Surgery
Universidade Federal de São Paulo
2015-2025
Associação Paulista de Medicina
2017
Fundação de Apoio à Universidade Federal de São Paulo
2017
The dependence of development and homeostasis in animals on the interaction hundreds extracellular regulatory proteins with peri- glycosaminoglycan heparan sulfate (HS) is exploited by many microbial pathogens as a means adherence invasion. Heparin, widely used anticoagulant drug, structurally similar to HS common experimental proxy. Exogenous heparin prevents infection range viruses, including S-associated coronavirus isolate HSR1. Here, we show that inhibits severe acute respiratory...
Abstract The dependence of the host on interaction hundreds extracellular proteins with cell surface glycosaminoglycan heparan sulphate (HS) for regulation homeostasis is exploited by many microbial pathogens as a means adherence and invasion. closely related polysaccharide heparin, widely used anticoagulant drug, which structurally similar to HS common experimental proxy, can be expected mimic properties HS. Heparin prevents infection range viruses if added exogenously, including...
Bone tissue is mineralized dense connective consisting mainly of a mineral component (hydroxyapatite) and an organic matrix comprised collagens, non-collagenous proteins proteoglycans (PGs). Extracellular PGs bind tightly to hydroxyapatite which would protect these molecules from the destructive effects temperature chemical agents after death. DNA have been successfully extracted archaeological skeletons valuable information has obtained; however, date neither nor glycosaminoglycan (GAG)...
Vesicle trafficking is pivotal in heparan sulfate (HS) biosynthesis, influencing its spatial and temporal regulation within distinct Golgi compartments. This modulates the sulfation pattern of HS, which crucial for governing various biological processes. Here, we investigate effects silencing Rab1A Rab2A expression on localisation 3-O-sulfotransferase-5 (3OST5) compartments subsequent alterations HS structure levels. Interestingly, led to a shift 3OST5 localization towards trans-Golgi,...
Calcitonin (CT) is a sensitive marker of medullary thyroid carcinoma (MTC) and used for primary diagnosis follow-up after thyroidectomy. However, persistently elevated CT observed even complete surgical removal without evidence recurrent or persistent tumor.To investigate the presence assay interference in serum MTC patients who are apparently structural disease.We studied three index cases 14 with metastatic MTC. The level was measured using an immunofluorometric assay. Screening performed...
3-O-Sulfotransferase enzyme (sHS) from Litopenaeus vannamei was cloned and its substrate specificity investigated against a number of GAG structures, including modified heparin polysaccharides model oligosaccharides. For the polysaccharides, derived porcine intestinal mucosa heparin, sulfate groups were incorporated into glucosamine residues containing both N-sulfated N-acetylated substitution within regions predominant repeating disaccharide, either I-ANS or I-ANAc. However, resulting did...
Biallelic pathogenic variants in TBCK cause encephaloneuropathy, infantile hypotonia with psychomotor retardation, and characteristic facies 3 (IHPRF3). The molecular mechanisms underlying its neuronal phenotype are largely unexplored. In this study, we reported two sisters, who harbored biallelic met diagnostic criteria for IHPRF3. We provided evidence that may play an important role the early secretory pathway neuroprogenitor cells (iNPC) differentiated from induced pluripotent stem...
Heparan sulfate (HS), a sulfated linear carbohydrate that decorates the cell surface and extracellular matrix, is ubiquitously distributed throughout animal kingdom represents key regulator of biological processes largely untapped reservoir potential therapeutic targets. The temporal spatial variations in HS structure underpin concept "heparanome" complex network binding proteins. However, despite its widespread roles, determination direct structure-to-function correlations impaired by...
Nowadays, pharmaceutical heparin is purified from porcine and bovine intestinal mucosa. In the past decade there has been an ongoing concern about safety of heparin, since in 2008, adverse effects associated with presence oversulfated chondroitin sulfate (OSCS) were observed preparations which led to death patients, causing a global public health crisis. However, it not clarified whether OSCS added preparation, or already introduced during production raw heparin. Using combination different...
Angiogenesis is the formation of new vessels from pre-existing vasculature. The heparan sulfate chains endothelial cell proteoglycans interact with major angiogenic factors, regulating blood vessels´ formation. Since FDA´s first approval, anti-angiogenic therapy has shown tumor progression inhibition and increased patient survival. Previous work in our group selected an HS-binding peptide using a phage display system. Therefore, we investigated effect angiogenesis progression. showed higher...
Xylella fastidiosa is a plant-infecting bacillus, responsible for many important crop diseases, such as Pierce's disease of vineyards, citrus variegated chlorosis, and coffee leaf scorch (CLS), among others. Recent genomic comparisons involving two CLS-related strains, belonging to X. subsp. pauca, revealed that one them carries frameshift mutation inactivates gene encoding an oxidoreductase the short-chain dehydrogenase/reductase (SDR) superfamily, which may play roles in determining...
Abstract The cell surface and extracellular matrix polysaccharide, heparan sulfate (HS) conveys chemical information to control or influence crucial biological processes. Attempts describe its structure-function relationships with HS binding proteins in a classical ‘lock key’ type manner, however, have been unsuccessful. chains are synthesized non-template driven process the ER Golgi apparatus, involving large number of enzymes capable fine-tuning structures. Changes localization...