Aberrant expression of transforming growth factor beta 1 (TGF-beta 1) has been implicated in a number disease processes, particularly those involving fibrotic and inflammatory lesions. To determine the vivo effects overexpression TGF-beta on function structure hepatic as well extrahepatic tissues, transgenic mice were generated containing fusion gene (Alb/TGF-beta consisting modified porcine cDNA under control regulatory elements mouse albumin gene. Five lines developed, all which expressed...

Transforming growth factor-type beta (TGF-beta) has been identified as a constituent of bone matrix (Seyedin, S. M., A. Y. Thompson, H. Bentz, D. M. Rosen, J. McPherson, Conti, N. R. Siegel, G. Gallupi, and K. Piez, 1986, Biol. Chem. 261:5693-5695). We used both developing bone-forming cells in vitro to demonstrate the cellular origin this peptide. TGF-beta mRNA was detected by Northern analysis tissue fetal bovine using human cDNA probes. shown be synthesized secreted metabolically labeled...

We have compared the expression of genes encoding transforming growth factors beta 1, 2 and 3 during mouse embryogenesis from 9.5 to 16.5 days p.c. using in situ hybridisation cellular RNAs. Each gene has a different pattern, which gives some indication possible biological function vivo. All three appear be involved chondroossification, though each is expressed cell type. Transcripts are also present embryonic epithelia. Epithelial TGF RNA associated with regions active morphogenesis...

We present evidence for unique localization and specific biological activities transforming growth factor-beta s (TGF-beta s) 2 3, as compared to TGF-beta 1, in the nervous system of 12-18 day mouse embryo. Each isoform was localized immunohistochemically by antibodies raised peptides corresponding sequences respective proteins. Staining 1 principally meninges, while 3 co-localized neuronal perikarya axons, well radial glial cells. In central system, staining most prominent zones where...

Journal Article cDNA cloning by PCR of rat transforming growth factor β-1 Get access Su Wen Qian, Qian * Laboratory Chemoprevention, National Cancer InstituteBethesda, MD 20892, USA To whom correspondence should be addressed Search for other works this author on: Oxford Academic PubMed Google Scholar Paturu Kondaiah, Kondaiah Anita B. Roberts, Roberts Micheal Sporn Nucleic Acids Research, Volume 18, Issue 10, 25 May 1990, Page 3059, https://doi.org/10.1093/nar/18.10.3059 Published: 1990...

ABSTRACT We have studied the expression of genes encoding transforming growth factors (TGFs) beta1, beta2 and beta3 during development secondary palate in mouse from 11.5 to 15.5 days postcoitum using situ hybridisation. The RNA detected at earliest developmental stage is TGF beta3, which localised epithelial component vertical palatal shelf. This continues horizontal shelf, predominantly medial edge epithelium, lost as seam disrupts, soon after shelf fusion. betal expressed with same...

Two sets of novel multiheaded cationic amphiphiles bearing one, two, and three trimethylammonium headgroups (T1, T2, T3) pyridinium (P1, P2, P3), have been synthesized tested for antimicrobial activities against both Gram-positive Gram-negative bacteria. The multicationic in these were attached covalently via scissile ester-type linkages. results compared with those known surface-active, nonhydrolyzable compounds cetyltrimethylammonium bromide (CTAB) cetylpyridinium (CPB). killing effects...

Transforming growth factorβ 1 (TGFβ 1) has been purified and the mRNA cloned from a number of mammalian species including human, murine, bovine, porcine, simian. Using human TGFβ cDNA probe, we have detected two distinct RNAs in cultured primary chick embryo chondrocytes. One these RNAs, migrating at about 1.7 kilobases, shows similarity to 1. The second RNA, 3 is novel which named 3. Clones corresponding each were isolated chondrocyte library. Two clones for 3, pTGFB-ChX17 pTGFB-ChX25,...

A novel transforming growth factor-beta (TGF-beta) mRNA of about 3.0 kilobases, which encodes a putative protein 382 amino acids, has been identified in amphibians by cDNA cloning. This mRNA, we designate as TGF-beta 5, is developmentally regulated and highly expressed beginning at early neurula (stage 14) many adult tissues Xenopus laevis. Following the first methionine, precursor hydrophobic region, approximately 22 acids long, probably represents signal sequence, similar to that found s...

Curcumin (Hcur) as a cellular imaging and PDT agent shows remarkable photocytotoxicity in HeLa cells visible light of 400–700 nm giving IC50 = 8.2 ± 0.2 μM its degradation is arrested on formation photocytotoxic dipyridophenazine (dppz) complex [VO(cur)(dppz)Cl] (IC50 3.3 0.4 μM), while both are less toxic the dark.

A red light for cancer cells: An iron(III) complex (1, see picture) that contains an anthracenyl fluorophore moiety and a catecholate ligand is potent, metal-based PDT agent efficiently photocleaves DNA in near-infrared light, has significant nuclear uptake, high photocytotoxicity by apoptotic pathway HeLa MCF-7 cells. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or typeset. They made available...

[Pt(cur)(NH3)2](NO3) (1), a curcumin-bound cis-diammineplatinum(II) complex, nicknamed Platicur, as novel photoactivated chemotherapeutic agent releases photoactive curcumin and an active platinum(II) species upon irradiation with visible light. The hydrolytic instability of free reduces binding to platinum(II). Interactions 1 5'-GMP ct-DNA indicated formation platinum-bound DNA adducts exposure light (λ=400-700 nm). It showed apoptotic photocytotoxicity in cancer cells (IC50 ≈ 15 μM), thus...

Oral submucous fibrosis (OSF) is a chronic inflammatory disease characterized by the accumulation of excess collagen, and areca nut chewing has been proposed as an important etiological factor for manifestation. Activation transforming growth factor-β signaling postulated main causative event increased collagen production in OSF. epithelium plays roles OSF, arecoline shown to induce TGF-β epithelial cells. In attempt understand role constituents manifestation we studied global gene...

Abstract We present herein a short tripeptide sequence (Lys–Phe–Gly or KFG) that is situated in the juxtamembrane region of tyrosine kinase nerve growth factor (Trk NGF) receptors. KFG self‐assembles water and shows reversible concentration‐dependent switching nanostructures from nanospheres (vesicles) to nanotubes, as evidenced by dynamic light scattering, transmission electron microscopy, atomic force microscopy. The morphology change was associated with transition secondary structure....

Stimuli-responsive and NIR fluorescence combination probe (<bold>QCy-BA</bold>⊂DNA) to efficiently quantify image normal elevated levels of hydrogen peroxide in primary disease-associated cells.

The term Laser "Photobiomodulation" was coined to encompass the pleiotropic effects of low-power lasers on biological processes. purpose this study investigate whether transforming growth factor (TGF)-beta had a role in mediating far-infrared laser irradiation. We assayed for vitro activation using various forms cell-secreted, recombinant, and serum latent TGF-beta p3TP reporter enzyme-linked immunosorbent assays. demonstrate here that are capable activating TGF-beta1 -beta3 and, further, it...

Abstract Background TGF-beta is one of the key cytokines implicated in various disease processes including cancer. inhibits growth and promotes apoptosis normal epithelial cells contrast, acts as a pro-tumour cytokine by promoting tumour angiogenesis, immune-escape metastasis. It not clear if actions on are due to differential gene regulations. Hence we studied regulation expression cancer cells. Results Using human 19 K cDNA microarrays, show that 1757 genes exclusively regulated A549...

The most important objective of the present study was to explain why cationic lipid (CL)-mediated delivery plasmid DNA (pDNA) is better than that linear in gene therapy, a question that, until now, has remained unanswered. Herein for first time we experimentally show different types CLs, pDNA, contrast DNA, compacted with large amount its counterions, yielding lower effective negative charge. This feature been confirmed through number physicochemical and biochemical investigations....

Nine lipopolymers based on low molecular weight polyethyleneimines (PEI) and cholesterol via an ether linkage between the polymer amine backbone have been synthesized. Different percentage of moieties grafted three types PEI weights 800, 1200, 2000. These were studied for gene transfection activities in HeLa cells. All first optimized enhanced efficacies as coliposomes with DOPE. are better transfecting agents highly serum compatible than commercially available PEI-25KDa. Transfection...

Lipoplexes formed by the pEGFP-C3 plasmid DNA (pDNA) and lipid mixtures containing cationic gemini surfactant of 1,2-bis(hexadecyl dimethyl ammonium) alkanes family referred to as C16CnC16, where n=2, 3, 5, or 12, zwitterionic helper lipid, 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) have been studied from a wide variety physical, chemical, biological standpoints. The study has carried out using several experimental methods, such zeta potential, gel electrophoresis, small-angle...