A5007291934- Polysaccharides and Plant Cell Walls
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Genetics and Neurodevelopmental Disorders
- Plant nutrient uptake and metabolism
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Cell Image Analysis Techniques
- Chromosomal and Genetic Variations
- Alzheimer's disease research and treatments
- Autism Spectrum Disorder Research
- Smart Agriculture and AI
- Plant and animal studies
- Legume Nitrogen Fixing Symbiosis
- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Plant Micronutrient Interactions and Effects
- Cellular transport and secretion
- Plant-Microbe Interactions and Immunity
- Insect Pest Control Strategies
Pennsylvania State University
2018-2024
Salk Institute for Biological Studies
2024
Beijing Institute of Technology
2017
Summary Herbivore‐induced plant volatiles (HIPVs) are widely recognized as an ecologically important defensive response of plants against herbivory. Although the induction this ‘cry for help’ has been well documented, only a few studies have investigated inhibition HIPVs by herbivores and little is known about whether evolved mechanisms to inhibit release HIPVs. To examine role herbivore effectors in modulating stomatal dynamics, we conducted series experiments combining pharmacological,...
Plant cell deformations are driven by pressurization and mechanical constraints imposed the nanoscale architecture of wall, but how these factors controlled at genetic molecular levels to achieve different types deformation is unclear. Here, we used stomatal guard cells investigate influences wall mechanics turgor pressure on demonstrate that expression pectin-modifying gene PECTATE LYASE LIKE12 (PLL12) required for normal dynamics in Arabidopsis thaliana. Using nanoindentation finite...
Stomatal pores are vital for the diffusion of gasses into and out land plants are, therefore, gatekeepers photosynthesis transpiration. Although much published literature has described intercellular signaling transcriptional regulators involved in early stomatal development, little is known about cellular details local separation between sister guard cells that give rise to pore or how formation this achieved. Using three-dimensional (3D) time-lapse imaging, we found Arabidopsis (Arabidopsis...
SUMMARY Stomata are pores at the leaf surface that enable gas exchange and transpiration. The signaling pathways regulate differentiation of stomatal guard cells mechanisms pore formation have been characterized in Arabidopsis thaliana . However, process by which complexes develop after into fully mature is poorly understood. We tracked morphogenesis young over time to establish characteristic geometric milestones along path maturation. Using 3D‐nanoindentation coupled with finite element...
Pectin is a critical component of the plant cell wall, supporting wall biomechanics and contributing to signaling in response stress. The carefully regulates pectin methylesterification with endogenous methylesterases (PMEs) their inhibitors (PMEIs) promote growth protect against pathogens. We expressed Aspergillus nidulans methylesterase (AnPME) Arabidopsis thaliana plants determine impacts status on function. Plants expressing AnPME had roughly 50% reduction methylester content compared...
Abstract The ability of plants to absorb CO 2 for photosynthesis and transport water from root shoot depends on the reversible swelling guard cells that open stomatal pores in epidermis. Despite decades experimental theoretical work, biomechanical drivers opening closure are still not clearly defined. We combined mechanical principles with a growing body knowledge concerning flux across plant cell membrane properties walls quantitatively test long-standing hypothesis increasing turgor...
Automating the three-dimensional (3D) segmentation of stomatal guard cells and other confocal microscopy data is extremely challenging due to hardware limitations, hard-to-localize regions, limited optical resolution. We present a memory-efficient, attention-based, one-stage neural network for 3D images cells. Our model trained end achieved expert-level accuracy while leveraging only eight human-labeled volume images. As proof concept, we applied our from cell ablation experiment that tests...
Stomata are pores at the leaf surface that enable gas exchange and transpiration. The signaling pathways regulate differentiation of stomatal guard cells mechanisms pore formation have been characterized in Arabidopsis thaliana. However, process by which complexes develop after into fully mature is poorly understood. We tracked morphogenesis young over time to establish characteristic geometric milestones along path maturation. Using 3D-nanoindentation coupled with finite element modeling...
Rett Syndrome is a neurological condition primarily affecting women. Its symptoms include decreased brain size, inability to speak, and coordination problems. Several gene mutations have been discovered play role in this disease, with the MeCP2 mutation being most widely known frequent cause. Scientists are also attempting create medicines targeting genetic that may provide cure. However, mechanism behind how affects human on molecular level remains be discovered. This article summarises...