A5007542925- Indoor Air Quality and Microbial Exposure
- Animal Disease Management and Epidemiology
- Insect symbiosis and bacterial influences
- Odor and Emission Control Technologies
- Genomics and Phylogenetic Studies
- Vector-Borne Animal Diseases
- Microbial Community Ecology and Physiology
- Bacterial Infections and Vaccines
- Research on Leishmaniasis Studies
- Viral Infections and Immunology Research
- Plant Pathogens and Fungal Diseases
- Genetics and Neurodevelopmental Disorders
- Antibiotic Resistance in Bacteria
- Air Quality and Health Impacts
- Insect and Pesticide Research
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Antibiotics Pharmacokinetics and Efficacy
- Reproductive tract infections research
- Autism Spectrum Disorder Research
- Insect behavior and control techniques
- Animal and Plant Science Education
- Microbial infections and disease research
- Insect Utilization and Effects
- Yeasts and Rust Fungi Studies
Instituto de Salud Carlos III
1998-2021
Hospital Universitario 12 De Octubre
2017-2021
Centro Nacional de Microbiologia
1998-2021
Instituto de Investigación de Enfermedades Raras
2016-2021
Centre for Biomedical Network Research on Rare Diseases
2016-2021
Centro de Investigación Biomédica en Red
2019-2021
European Union Satellite Centre
2015-2018
Centro de Investigación en Sanidad Animal
2008-2018
University of Milan
2013-2018
Federal Institute for Occupational Safety and Health
2009-2017
This study represents a complete comparative analysis of the most widely used African swine fever (ASF) diagnostic techniques in European Union (EU) using field and experimental samples from animals infected with genotype II ASF virus (ASFV) isolates circulating Europe. To detect ASFV, three different PCRs were evaluated parallel 785 samples. The results showed almost perfect agreement between Universal ProbeLibrary (UPL-PCR) real-time (κ = 0.94 [95% confidence interval {CI}, 0.91 to 0.97])...
Feed security, feed quality and issues surrounding the safety of raw materials are always interest to all livestock farmers, manufacturers competent authorities. These concerns even more important when alternative ingredients, new product developments innovative feeding trends, like insect-meals, considered. The black soldier fly (Hermetia illucens) is considered a good candidate be used as ingredient for aquaculture other farm animals, mainly an protein source. Data on transfer contaminants...
The role of the ancestral sylvatic cycle African swine fever virus (ASFV) is not well understood in endemic areas eastern Africa. We therefore analysed ASF infection status on samples collected from 51 free-ranging warthogs (Phacocherus africanus) and 1576 Ornithodorus porcinus ticks 26 independent warthog burrows at a single ranch Kenya. Abattoir 83 domestic pigs without clinical symptoms, originating specific locations with no recent reported outbreaks were included this study. All derived...
Abstract Background The Midichloriaceae is a novel family of the order Rickettsiales, that encompasses intracellular bacteria associated with hard ticks (Ixodidae) and other arthropods. most intensively investigated member this Midichloria mitochondrii , symbiotic bacterium sheep tick Ixodes ricinus characterized by capacity multiplying inside mitochondria. A recent study suggested these might be inoculated into human host during bite. purpose was to determine potential infectivity for...
ObjectivesTo determine trends in ciprofloxacin resistance and co-resistance to other antibiotic classes blood isolates of Escherichia coli, investigate if there is an ecological relationship the community use fluoroquinolones antibiotics.
Employees at agricultural working places are often exposed to complex bioaerosols. Investigations of bioaerosols in duck houses revealed concentrations cultivable bacteria between 0.4 and 3 x 10(5) colony forming units (CFU) m(-3) on tryptone soy agar, 0.3 2 CFU actinomycetes isolation 0.8 5 10(3) Middlebrook respectively, when incubated 25 degrees C. At an incubation temperature 37 C, 0.6-3 10(2) were counted MacConkey agar 0.3-2 the glycerol-arginine oatmeal 50 C varied 0.1 1 7 m(-3),...
Summary There is still a lack of studies on fungal microbiota in mosquitoes, compared with the number available bacterial microbiota. This study reports identification yeasts clinical significance laboratory mosquito species: Anopheles gambiae , stephensi Culex quinquefasciatus Aedes albopictus and aegypti . Among isolated, they focused opportunistic pathogen Candida parapsilosis since there need to better understand breakthrough candidaemia resistance usual antifungals, which requires...
A Gram-positive, non-spore-forming actinobacterium (Sj 10(T)) was isolated on tryptone soy agar from the air of a duck barn after filter sampling. Based 16S rRNA gene sequence similarity studies, strain Sj 10(T) shown to belong genus Leucobacter and closely related chromiireducens subsp. L-1(T) (97.8 %), tardus DSM 19811(T) (97.3 %) luti RF6(T) %). The peptidoglycan contained 2,4-diaminobutyric acid in combination with lower amount lysine as diagnostic diamino acids. In addition, threonine,...
Analysis of the 16S rRNA gene sequences type strains Aquamicrobium defluvii and Defluvibacter lusatiensis shows a similarity 98.3 %. There is no evidence for clear phenotypic differences between these organisms that justifies assignment to different genera. Both have ubiquinone Q-10 as dominant quinone very similar fatty acid profiles, with 18 : 1ω7c 19 0 cyclo ω8c predominating. A proposal made transfer genus lusatiense comb. nov. (type strain S1T =CIP 106844T =DSM 11099T). Furthermore,...
Abstract Yeasts of the Meyerozyma guilliermondii species complex are widespread in nature and can be isolated from a variety sources, environment to arthropods hospital patients. To date, comprises thoroughly studied versatile M. guilliermondii, hard distinguish caribbica, Candida carpophila. Here we report whole genome sequencing de novo assembly four caribbica isolates, identified with most recent molecular techniques, derived Diptera species. The novel assemblies present reduced...
Abstract Rett syndrome (RTT) is an early-onset neurodevelopmental disorder that almost exclusively affects girls and totally disabling. Three genes have been identified cause RTT: MECP2 , CDKL5 FOXG1 . However, the etiology of some RTT patients still remains unknown. Recently, next generation sequencing (NGS) has promoted genetic diagnoses because quickness affordability method. To evaluate usefulness NGS in diagnosis, we present study RTT-like using different techniques based on this...
A Gram-stain-negative, rod-shaped, oxidase-positive, non-spore-forming bacterium (Sa15(T)) was isolated from the air in a duck barn. On basis of 16S rRNA gene sequence similarity studies, organism grouped into class gammaproteobacteria neighbourhood genus Fulvimonas. The quinone system consisted exclusively ubiquinone Q-8. polar lipid profile mainly composed major lipids diphosphatidylglycerol, phosphatidylmonomethylethanolamine and phosphatidylcholine, moderate amounts phosphatidylglycerol...
A Gram-negative, rod-shaped, oxidase-positive, non-spore-forming, non-motile bacterium (Sa25(T)) was isolated from air of a duck barn. 16S rRNA gene and recA sequence analyses clearly placed the isolate in vicinity Brucella-Ochrobactrum-Pseudochrobactrum group, with closest relative being Pseudochrobactrum glaciei KMM 3858(T). This allocation confirmed by quinone system (ubiquinone Q-10), fatty acid data (major acids C(18 : 1)omega7c C(19 0) cyclo omega8c) polar lipid profile components...
A Gram-stain-positive, coccoid, non-endospore-forming actinobacterium (Sj14a T ) was isolated from the air of a duck barn on tryptone soy agar after filter sampling. On basis 16S rRNA gene sequence similarity studies, strain Sj14a shown to belong genus Dietzia and most closely related schimae (98.7 %), cercidiphylli (98.4 %) maris (98.6 %). The major menaquinone MK-8 (H 2 ). polar lipid profile included components diphosphatidylglycerol, phosphatidylglycerol, an unknown two glycolipids....
Microbial symbionts played a central role in insect evolution. Oreina cacaliae (Schrank, 1785) (Coleoptera: Chrysomelidae) is rare example of viviparous insect, able to feed on toxic plants and sequester compounds. In the current study, microbiota associated with O. was characterized using culture-independent approach, targeting 16S rRNA bacterial gene. The obtained gene sequences were analyzed identified at different taxonomic levels. Wolbachia dominant bacterium, both male female (100...
Abstract Rett syndrome (RTT) is a severe neurological disorder usually caused by mutations in the MECP2 gene. Since gene located on X chromosome, chromosome inactivation (XCI) could play role wide range of phenotypic variation RTT patients; however, classical methylation-based protocols to evaluate XCI not determine whether preferentially inactivated carried mutant or wild-type allele. Therefore, we developed an allele-specific assay methylation at loci several recurrent mutations. We...
Today's large-scale poultry production is often accompanied by high concentrations of airborne microorganisms at working places. However, the microbial communities in those bioaerosols are rarely characterised. In this study, we investigated bacterial population from a duck hatchery both cultivation based and molecular methods compared results. Depending on used media, culturable bacteria varied between 6 × 10(1) 7 10(6) CFU per m(3) air. The corresponding total cell count DAPI stained cells...