A5064665433- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Carbon and Quantum Dots Applications
- Advanced Fluorescence Microscopy Techniques
- Luminescence and Fluorescent Materials
- Orbital Angular Momentum in Optics
- Molecular Sensors and Ion Detection
- Advanced Nanomaterials in Catalysis
- CRISPR and Genetic Engineering
- Nanocluster Synthesis and Applications
- Quantum Dots Synthesis And Properties
- Gold and Silver Nanoparticles Synthesis and Applications
- DNA and Nucleic Acid Chemistry
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- Microfluidic and Capillary Electrophoresis Applications
- Analytical Chemistry and Sensors
- Spectroscopy Techniques in Biomedical and Chemical Research
- Electrochemical sensors and biosensors
- Single-cell and spatial transcriptomics
- Graphene and Nanomaterials Applications
- Soil erosion and sediment transport
Wuhan University
2016-2025
Hohai University
2009-2024
State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering
2010-2024
Ministry of Water Resources of the People's Republic of China
2024
Wuhan Institute of Bioengineering
2012-2020
State Council of the People's Republic of China
2020
Guilin University of Electronic Technology
2008-2018
Central South University
2012-2018
State Key Laboratory of Virology
2010-2015
Zhongnan Hospital of Wuhan University
2010
Mucin 1 (MUC1) which presents in epithelial malignancies, is a well-known tumor biomarker. In this paper, highly sensitive and selective fluorescent aptasensor for detection constructed, utilizing graphene oxide (GO) as quencher can quench the fluorescence of single-stranded dye-labeled MUC1 specific aptamer. absence MUC1, adsorption aptamer on GO brings dyes close proximity to surface resulting high efficiency quenching dye fluorescence. Therefore, designed completely quenched by GO, system...
Taking advantage of outstanding precision in target recognition and trans-cleavage ability, the recently discovered CRISPR/Cas12a system provides an alternative opportunity for designing fluorescence biosensors. To fully exploit analytical potential, we introduce here some meaningful concepts. First, collateral cleavage is efficiently activated a functional DNA regulation manner bottleneck which largely applicable to nucleic acids detection broken. After selection representative aptamer...
We report on the capabilities of near-infrared surface-enhanced Raman scattering (SERS) using gold nanoparticles to obtain detailed chemical information with high spatial resolution from within single cancer cells, living or fixed. Colloidal particles, 60 nm in size, were introduced into live human osteosarcoma cells by endocytosis adding them growth medium. Rapid SERS mapping indicated that not only could rich vibrational spectra be obtained intrinsic cellular constituents both cytoplasm...
The further development of high-performance fluorescent biosensors to image intracellular microRNAs is beneficial cancer medicine. By virtue the need for enzymes and hairpin DNA probes, entropy-driven reaction-assisted signal amplification strategy has shown an enormous potential accomplish this task. Nevertheless, good option still meets with poor biostability, low cell uptake efficiency, unsatisfactory accuracy. On basis these challenges, we put forward here a battery solving pathways....
Although great headway has been made in DNAzyme-based detection of Pb2+, its adaptability, sensitivity, and accessibility complex media still need to be improved. For this, we introduce new ways surmount these hurdles. First, a spherical nucleic acid (SNA) fluorescence probe (Au nanoparticles-DNAzyme probe) is utilized specifically identify Pb2+ suitability for precise samples due excellent nuclease resistance. Second, the sensitivity greatly enhanced via use clustered regularly interspaced...
The CRISPR-Cas system has been found to be extremely sensitive and there is an urgent demand extend its potential in bioassays.
Abstract This study presents a linear charge-coupled device (CCD) spectrometer and its application to quantitative determination of rutin with UV-Vis spectrophotometry fluorimetry detections. The can obtain spectrum the range 271.44 nm from one exposure 1.7 resolution. With same spectrometer, in tablet was successfully studied by measuring absorption laser-induced fluorescence emission. data shows that there is no significant difference for determining between two methods. result implies...
We explore the interactions between a fluorescein (FAM)-labeled single-stranded DNA (P), graphene oxide (GO), and cationic conjugated polymer, poly [(9,9-bis(6'-N,N,N-trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP). It is found that fluorescence change of P-GO-PFP system dependent on addition order P PFP. When adding PFP into P/GO complex, resonance energy transfer (FRET) from to inefficient. If added PFP/GO efficient FRET obtained. This may be attributed equal binding...
A fluorescence method for protein detection is developed based on terminal protection of small-molecule-linked DNA by target and a graphene oxide-assisted assay strategy. This design results in fluorescence-enhanced that sensitive selective the protein. As service to our authors readers, this journal provides supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical support issues arising from...
Membrane nanotubes can facilitate direct intercellular communication between cells and provide a unique channel for transfer of cellular contents. However, the transport mechanisms membrane remain poorly understood cancer cells. Also largely unknown is pattern mediated by nanotubes. In this work, wheat germ agglutinin (WGA), widely used drug carrier potential antineoplastic drug, was labeled with quantum dots (QDs-WGA) as model exploring transportation via We found that allowed effective...
The mechanism for short ssDNA having weaker affinity to graphene oxide than long was systematically investigated.
Abstract Comprehensive phenotypic profiling of heterogeneous circulating tumor cells (CTCs) at single‐cell resolution has great importance for cancer management. Herein, a novel spectrally combined encoding (SCE) strategy was proposed multiplex biomarker single CTCs using multifunctional nanosphere‐mediated microfluidic platform. Different cellular biomarkers uniquely labeled by nanosphere barcodes, possessing identical magnetic tags and distinct optical signatures, enabled isolation with...
While three-dimensional (3D) DNA walking amplifiers hold considerable promise in the construction of advanced DNA-based fluorescent biosensors for bioimaging, they encounter certain difficulties such as inadequate sensitivity, premature activation, need exogenous propelling forces, and low reaction rates. In this contribution, a variety profitable solutions have been explored. First, catalytic hairpin assembly (CHA)-achieved nonenzymatic isothermal nucleic acid amplification is integrated to...
Breast cancer (BC) is a heterogeneous tumor, and better understanding of its heterogeneity essential to improving treatment effect. Quantum dot (QD)-based immunofluorescent nanotechnology (QD-IHC) for molecular pathology has potential advantages in delineating tumor heterogeneity. This explored this paper by QD-IHC imaging HER2 ER. BC can be displayed more clearly sensitively than conventional IHC tissue microarrays. Furthermore, the simultaneous ER might help understand their interactions...