A5083803152- Geophysics and Gravity Measurements
- Ionosphere and magnetosphere dynamics
- Solar and Space Plasma Dynamics
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Ubiquitin and proteasome pathways
- Graphene research and applications
- RNA Research and Splicing
- Cancer Immunotherapy and Biomarkers
- Breast Cancer Treatment Studies
- Cancer Cells and Metastasis
- Biosensors and Analytical Detection
- Pesticide Residue Analysis and Safety
- Autophagy in Disease and Therapy
- Molecular Biology Techniques and Applications
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Analytical Chemistry and Chromatography
- Breast Lesions and Carcinomas
Qilu Hospital of Shandong University
2017-2025
Capital Medical University
2024-2025
Beijing Shijitan Hospital
2024-2025
Yancheng Institute of Technology
2025
Shandong University
2017-2024
Brigham Young University
2018-2024
Capital University
2024
Zhongshan Hospital
2024
Fudan University
2024
University of Science and Technology Beijing
2019-2023
Tamoxifen is the most commonly used endocrine therapy for patients with hormone receptor (HR)-positive breast cancer. Despite its initial therapeutic efficacy, many eventually develop drug resistance, which remains a serious clinical challenge. To investigate roles of circular RNAs (circRNAs) in tamoxifen tamoxifen-resistant MCF-7 cell line was established and screened circRNA expression profile by RNA sequencing. hsa_circ_0025202, that significantly downregulated, selected further...
Abstract Background Long noncoding RNAs (lncRNAs) play crucial roles in tumor progression and are aberrantly expressed various cancers. However, the functional of lncRNAs breast cancer remain largely unknown. Methods Based on public databases integrating bioinformatics analyses, overexpression lncRNA BCRT1 tissues was detected further validated a cohort tissues. The effects proliferation, migration, invasion macrophage polarization were determined by vitro vivo experiments. Luciferase...
Abstract Rapid proliferation and metastasis of breast cancers resulted in poor prognosis clinic. Recent studies have proved that long noncoding RNAs (lncRNAs) were involved tumor progression. In this study, we aimed to determine the roles mechanisms lncRNA–cell division cycle 6 (CDC6) regulating cancer. Clinically, lncRNA–CDC6 was highly expressed tissues positively correlated with clinical stages cancers. Functionally, ectopic expression promoted via regulation G1 phase checkpoint, further...
The combination of nanodroplet sample preparation, ultra-low-flow nanoLC, high-field asymmetric ion mobility spectrometry (FAIMS) and latest-generation mass instrumentation provides dramatically improved single-cell proteome profiling.
Disruption of the gut microbiota homeostasis may induce low-grade inflammation leading to obesity-associated diseases. A major protective mechanism is use multi-layered mucus structures keep a safe distance between epithelial cells and microbiota. To investigate whether pesticides would insulin resistance/obesity through interfering with mucus-bacterial interactions, we conducted study determine how long-term exposure chlorpyrifos affected C57Bl/6 CD-1 (ICR) mice fed high- or normal-fat...
Single-cell proteomics can provide unique insights into biological processes by resolving heterogeneity that is obscured bulk measurements. Gains in the overall sensitivity and proteome coverage through improvements sample processing analysis increase information content obtained from each cell, particularly for less abundant proteins. Here we report on improved single-cell combination of previously developed nanoPOTS platform with further miniaturization liquid chromatography (LC)...
The tumor microenvironment contributes to progression and metastasis. Cancer-associated fibroblasts (CAFs) form a major cellular component of the microenvironment. In this study, we further explored mechanisms underlying tumor-promoting roles CAFs. Methods: Patient-derived CAFs normal (NFs) were isolated from breast carcinomas adjacent tissue. Exosomes by ultracentrifugation CAF-derived exosomal microRNAs screened using next-generation sequencing technology. MiR-500a-5p expression was...
The protein-coding ability of circular RNAs (circRNAs) has recently been a hot topic, but the expression and roles circRNAs in triple-negative breast cancer (TNBC) remain uncertain. By intersecting circRNA sequencing data from clinical samples cell lines, we identified circRNA, termed circ-EIF6, which predicted poorer prognosis correlated with clinicopathological characteristics cohort TNBC patients. Functionally, showed that circ-EIF6 promoted proliferation metastasis cells vitro vivo....
Chemoresistance is one of the major causes therapeutic failure and poor prognosis for breast cancer patients, especially triple-negative patients. However, underlying mechanism remains elusive. Here, we identified novel functional roles heat shock protein beta-1 (HSPB1), regulating chemoresistance ferroptotic cell death in cancer. Based on TCGA GEO databases, HSPB1 expression was upregulated tissues associated with which considered an independent prognostic factor Functional assays revealed...
A limitation of conventional bulk-tissue proteome studies in amyotrophic lateral sclerosis (ALS) is the confounding motor neuron (MN) signals by admixed non-MN proteins. Here, we leverage laser capture microdissection and nanoPOTS single-cell mass spectrometry-based proteomics to query changes protein expression single MNs from postmortem ALS control tissues. In a follow-up analysis, examine impact stratification based on cytoplasmic transactive response DNA-binding 43 (TDP-43)+ inclusion...
Abstract Chemo-resistance and metastasis of triple negative breast cancer (TNBC) contributed the most treatment failure in clinic. MicroRNAs (miRNAs) have been proved to be involved many biological processes diseases. In this study, we aimed determine role miR-770 regulation chemo-resistance TNBC. Clinically, was highly expressed chemo-sensitive tissues predicted a better prognosis Functionally, ectopic expression suppressed doxorubicin-resistance TNBC cell lines via apoptosis tumor...
We report reduced contact resistance of single-layer graphene devices by using ultraviolet ozone (UVO) treatment to modify the metal/graphene interface. The were fabricated from mechanically transferred, chemical vapor deposition (CVD) grown, single layer graphene. UVO in regions as defined photolithography and prior metal was found reduce interface contamination originating incomplete removal poly(methyl methacrylate) (PMMA) photoresist. Our control experiment shows that exposure times up...
Current mass spectrometry (MS)-based proteomics approaches are ineffective for mapping protein expression in tissue sections with high spatial resolution because of the limited overall sensitivity conventional workflows. Here we report an integrated and automated method to advance spatially resolved by seamlessly coupling laser capture microdissection (LCM) a recently developed nanoliter-scale sample preparation system termed nanoPOTS (Nanodroplet Processing One pot Trace Samples). The...
Abstract Triple-negative breast cancer (TNBC) is highly heterogeneous and has a poor prognosis. It therefore important to identify the underlying molecular mechanisms in order develop novel therapeutic strategies. Although emerging research revealed long noncoding RNAs (lncRNA) as vital carcinogenesis progression, their functional involvement TNBC not been well defined. In this study, we utilized The Cancer Genome Atlas (TCGA) database analyzed clinical samples show that antisense transcript...
Recent advances in sample preparation and analysis have enabled direct profiling of protein expression single mammalian cells other trace samples. Several techniques to prepare analyze low-input samples employ custom fluidics for nanoliter processing manual injection onto a specialized separation column. While being effective, these highly systems require significant expertise fabricate operate, which has greatly limited implementation most proteomic laboratories. Here, we report fully...
Single-cell proteomics (SCP) has great potential to advance biomedical research and personalized medicine. The sensitivity of such measurements increases with low-flow separations (<100 nL/min) due improved ionization efficiency, but the time required for sample loading, column washing, regeneration in these systems can lead low measurement throughput inefficient utilization mass spectrometer. Herein, we developed a two-column liquid chromatography (LC) system that dramatically label-free...
We combined efficient sample preparation and ultra-low-flow liquid chromatography with a newly developed data acquisition analysis scheme termed wide window (WWA) to quantify >3,000 proteins from single cells in rapid label-free analyses. WWA employs large isolation windows intentionally co-isolate co-fragment adjacent precursors along the selected precursor. Optimized increased number of MS2-identified by ≈40 % relative standard data-dependent acquisition. For 40-min LC gradient operated at...
Abstract Triple‐negative breast cancer (TNBC) is a highly aggressive subtype of that suggested to be associated with hypoxia. This study the first identify novel circular RNA (circRNA), circTBC1D14, whose expression significantly upregulated in TNBC. The authors confirm high circTBC1D14 poor prognosis patients cancer. circTBC1D14‐associated mass spectrometry and RNA‐binding protein‐related bioinformatics strategies indicate FUS can interact which bind downstream flanking sequence induce...
Mitophagy, which selectively eliminates the dysfunctional and excess mitochondria by autophagy, is crucial for cellular homeostasis under stresses such as hypoxia. Dysregulation of mitophagy has been increasingly linked to many disorders including neurodegenerative disease cancer. Triple-negative breast cancer (TNBC), a highly aggressive subtype, reported be characterized However, role in hypoxic TNBC well underlying molecular mechanism largely unexplored. Here, we identified GPCPD1...
Abstract Breast cancer is the major common malignancy worldwide among women. Previous studies reported that cancer-associated fibroblasts (CAFs) showed pivotal roles in regulating tumor progression via exosome-mediated cellular communication. However, detailed mechanism underlying exosomal circRNA from CAFs breast remains ambiguous. Here, profiling of cancer-derived and normal (NFs) was detected by high-throughput sequencing, upregulated circTBPL1 expression identified CAF exosomes. The...
Recent developments in mass spectrometry-based single-cell proteomics (SCP) have resulted dramatically improved sensitivity, yet the relatively low measurement throughput remains a limitation. Isobaric and isotopic labeling methods been separately applied to SCP increase through multiplexing. Here we combined both forms of achieve multiplicative scaling for higher throughput. Two-plex stable isotope amino acids cell culture (SILAC) isobaric tandem tag (TMT) enabled up 28 single cells be...