Variant sublines LMF3, LMF4 and LMF5 with high metastatic potential were established from a human oral squamous carcinoma cell line HSC‐3. These metastasized to the draining lymph nodes after subcutaneous inoculation into nude mice. They obtained by sequential selection in vivo parent At each step, cells which cultured reinoculated Two other lines HSC‐2 HSC‐4 also patients, that had neither invasive nor potential. Biologic properties compared among metastatic, low non‐metastatic cells....

Calcium, but not magnesium or strontium ions, specifically induce the precipitation of dentin phosphoprotein, which is reversibly solubilized by EDTA. This finding very useful in pruifying phosphoprotein either a free matrix-bound type. Bovine phosphoproteins, thus isolated, contain minimal amount contaminant protein, unlike previous preparations.

Previous studies by the authors demonstrated that response of urinary aquaporin-2 (AQP2) excretion to dDAVP (deamino-8-D-arginine vasopressin) infusion is an index vasopressin action on kidney (N Engl J Med 332: 1540-1545, 1995). In study presented here, characteristics AQP2 were examined further. An RIA suitable for in urine was established. Relatively high concentrations detergent and bovine serum albumin buffer allowed analysis samples with a wide range increased sensitivity assay....

The physiological function of the prion protein (PrP) remains enigmatic despite its established involvement in pathogenesis spongiform encephalopathies. PrP is a glycolipid-anchored membrane protein, which constitutively recycles between cell surface and an endosomal compartment. N-terminal region contains four tandem repeat (OP4) octapeptide PHGGGWGQ (OP) that binds reduces Cu(II) ions. We have examined kinetic properties OP4-mediated reduction found OP4 exhibits highest activity around pH...

We propose a novel ethylenediamine plasma-polymerized film matrix that is deposited on gold surfaces for use in surface plasmon resonance immunosensing. The films formed the are extremely thin (∼100 nm), homogeneous, demonstrate good adhesion, have flat profile, and incorporate amino groups to introduce chemical functionality. A sensor chip made with has many advantages when compared conventionally used designs such as carboxylated dextran hydrogels. For example, shows better response than...

Dentin phosphophoryn is a highly phosphorylated protein which has hydrophilic character but not soluble in dilute acetic acid. A histochemical method was developed for staining this with Stains‐all® situ utilizing those chemical properties. We have succeeded detecting the presence of circumpulpal orthodentin human permanent and deciduous teeth, mantle dentin, secondary dentin reparative dentin. Phosphophoryn also absent dentinogenesis imperfecta (DI) Type II, genetic disorder formation. From...

Comparative investigations of odontogenic cells in normally forming teeth and tumors may provide insights into the mechanisms differentiation process. The present study is devoted to late phenotypic markers ameloblast odontoblast cells, i.e., proteins involved biomineralization. situ expression amelogenins, keratins, collagens type III IV, vimentin, fibronectin, osteonectin, osteocalcin was performed on normal tumor human cells. pattern protein showed some similarities between ameloblasts...

Molecular cloning of a bovine amelogenin cDNA was accomplished by construction expression library (λgt11 library) from the ameloblast mRNA and then screening with antibodies to amelogenins. The complete primary structure an deduced cloned cDNA. One clones isolated phage λgt11 had 864-base-pair-long insert that encoded protein 216 amino acid residues. This clone appears represent coding region mRNA, including putative AUG initiation codon signal peptide sequence. predicted sequence has 87%...

Aquaporin-2 (AQP-2) is an arginine vasopressin (AVP)-regulated water channel in kidney collecting duct cells. The present study was undertaken to determine whether a change tonicity could directly regulate the AQP-2 gene vitro experiment.Various fragments of 5'-flanking region murine up -9.5 kb were cloned into luciferase (Luc) reporter plasmid, and they transiently transfected Madin-Darby canine cells.Hypertonicity significantly increased Luc activity constructs containing >6.1 (-6.1AQP2)....

Phosphophoryn, the most abundant of dentin non-collagenous proteins, has been considered to be related in function mineralization process. In present study, identification phosphophoryn localization was attempted using newly developed, precautionary histological methods by which retained sections during specimen preparation and stained selectively situ. Phosphophoryn found widely all calcified except mantle dentin, external, first-formed portion but not predentin, inner, uncalcified layer...

The age-dependent changes in reducible collagen cross-links, Schiff bases and their precursors were followed for human bone articular cartilage throughout the whole life span from 3 to 89 years of age. With aging, reduced dihydro-ylysinonorleucine remarkably decreased, whereas hexitollysine peaks increased significantly both collagens. Dihydroxynorleucine, precursor cross-link, was found increase slightly with But hydroxylysinonorleucine lysinonorleucine comparatively small. These discussed...

To investigate the hypothesis that there is difference in treatment outcomes of milder skeletal Class III malocclusion between facemask and combination with a miniscrew growing patients.Patients were randomly divided into two groups. In one group, patients treated therapy (FM group: 12 males, eight females, average age: 10 years, 5 months ± 1 year, 8 months). other along (FM+MS seven 11 month 3 A lingual arch hooks was fixed to maxillary both groups protractive force 500 g applied from...

The sensitivities of surface plasmon resonance spectroscopy were examined at incident angles 66–76 deg. calculated for various refractive indices liquid samples and thicknesses deposited dielectric thin layers. Furthermore, the confirmed experimentally. experimentally measured 1.3311–1.3463 0–89 nm, respectively. From these results it was demonstrated that sensing system showed higher with smaller angles. For example, sensitivity a index 66° angle seven times larger than 76°. It also...

Fetuin, an abundant protein in fetal bovine plasma, is the homolog of human alpha 2HS glycoprotein (alpha 2HS). In spite numerous studies, biological functions both proteins remain elusive. We now report remarkable 45Ca-binding activity fetuin serum that has been blotted on a membrane after electrophoresis. The Ca-binding ability purified was analyzed by equilibrium dialysis, which revealed had multiple sites, one Kd 0.95 x 10(-4) M. It also shown greater than albumin fetus at late...

Enzymatic and immunological properties of alkaline phosphatase [ALP; orthophosphoric monoester phosphohydrolase, optimum, EC 3.1.3.1.] in the human dental pulp were investigated. In inhibition thermal inactivation studies, ALP showed universal-type (kidney/bone/liver type). Dental cross-reacted with polyclonal monoclonal antibodies against purified swine-kidney ALP, antibody osteoblast-like cells same manner as ALPs bone kidney. The sodium dodecyl sulfate-gel electrophoretic pattern a 140,...

Abstract A pathogenic agent isolated in mice from the brain of a sheep affected by scrapie‐like disease was characterized. The incubation period primary transmission to longer than secondary and tertiary same strain mice. Progressive dilution inoculum caused prolongation period. infectivity 10% homogenate persisted, but decreased about 10 3 4 times after heating at 100 C for 30 min. Histological changes diseased mouse brains consisted vacuolation nerve cells spongiform degeneration gray...