A5072249403- Microbial bioremediation and biosurfactants
- Microbial Metabolic Engineering and Bioproduction
- Microbial metabolism and enzyme function
- Electrochemical sensors and biosensors
- Fungal Biology and Applications
- Mass Spectrometry Techniques and Applications
- Amino Acid Enzymes and Metabolism
- Biocrusts and Microbial Ecology
- Advanced Chemical Sensor Technologies
- Electrochemical Analysis and Applications
- bioluminescence and chemiluminescence research
- Chemical Reactions and Isotopes
- Analytical Chemistry and Sensors
- Metal-Catalyzed Oxygenation Mechanisms
- Protist diversity and phylogeny
- Biopolymer Synthesis and Applications
- Nonlinear Optical Materials Studies
- Photorefractive and Nonlinear Optics
- Microfluidic and Capillary Electrophoresis Applications
- Biochemical Acid Research Studies
- Sesame and Sesamin Research
- Digital Economy and Transformation
- Microbial Metabolism and Applications
- Ion Transport and Channel Regulation
- Material Science and Thermodynamics
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
2015-2024
The Russian Presidential Academy of National Economy and Public Administration
2020
Institute of Engineering Physics
1998
Biodegradation of phenol is an effective method for removing this toxicant from contaminated sites. Phenol a toxic compound living cells, so many bacteria degrade in relatively low concentrations, up to 0.75 g L−1. The Rhodococcus opacus strain 1CP destructor wide range pollutants. In the absence carbon source medium, cells R. easily form cyst-like resting (CLC). purpose work was evaluate viability during long-term storage and efficiency process destruction by germinating after dormancy....
Bacteria make a huge contribution to the purification of environment from toxic stable pollutants anthropogenic and natural origin due diversity their enzyme systems. For example, ability decompose 3-chlorobenzoate (3CBA) by four representative genera Actinobacteria, such as Rhodococcus, Gordonia, Microbacterium, Arthrobacter, was studied. In most cases, formation 4-chlorocatechol only key intermediate during decomposition 3CBA observed. However, Rhodococcus opacus strain 1CP an exception,...
The process of benzoate degradation by strain Rhodococcus opacus 1CP after a five-year dormancy was investigated and its peculiarities were revealed. shown to be capable growth on at concentration up 10 g L−1. substrate specificity dioxygenase (BDO) during the culture medium with low (200–250 mg L−1) high (4 assessed. BDO R. an extremely narrow enzyme. Out 31 substituted benzoates, only one, 3-chlorobenzoate, activity higher than 9% that benzoate. Two dioxygenases, catechol 1,2-dioxygenase...
Actuality of the innovational potential formation regions was revealed in article. Problems development Central Federal District were emphasized. Volume indexes goods, works, services, charges for innovations distinguished. Reasons differences activity estimated. The leading shown, and dynamic index changes presented as well. On basis performed analysis it revealed, that both objective factors, are connected with geographic locations, source raw materials, operation large enterprises,...
In the process of evolution, living organisms develop mechanisms for population preservation to survive in unfavorable conditions. Spores and cysts are most obvious examples dormant forms microorganisms. Non-spore-forming bacteria also capable surviving conditions, but patterns their behavior adaptive reactions have been studied less detail compared spore-forming organisms. The purpose this work was study features transition from dormancy active vegetative growth one non-spore-forming bacteria,
The aim of present study was to describe the graphical technique how go from Hill concentration constant Michaelis constant. To compare enzymatic processes, kinetics which is subjected different regularities, it possible use constants that characterize catalytic activity (Vmax) and are substrate at rate process equal a half maximum permissible rate. Concentration S0.5 for equation Km Michaelis-Menton equation. approach proposed in order could be characterized by same (the values minimum...
The electrochemical reactor microbial sensor with the Clark oxygen electrode as transducer was used for investigation of competition between 3-chlorobenzoate (3-CBA) and its analogues, 2- 4-chlorobenzoate (2-CBA 4-CBA), 3-chlorobenzoate-1,2-dioxygenase (3-CBDO) Rhodococcus opacus 1CP cells. change in respiration freshly harvested R. cells response to 3-CBA served an indicator 3-CBDO activity. results obtained confirmed inducibility 3-CBDO. Sigmoidal dependency rate enzymatic reaction on...
Abstract We explored the effect of a change in substrate-benzoate (as sole carbon and energy source) concentration growth medium on activity benzoate 1,2-dioxygenase (BDO) R.opacus 1CP cells, where BDO is enzyme mediated initial attack benzoate. The was estimated by respiration whole freshly harvested bacterial cells (growth benzoate) response to injection It shown that when decreased from 6 g/L 250 mg/L, curves dependency rate demonstrated kinetics process changes hyperbolic saturation...
Microbial reactor sensors (based on freshly harvested intact microbial cells) or membrane immobilized can be used as convenient instruments for studying processes that cause the response of a biosensor, such properties enzymes characteristics metabolism. However, mechanisms formation biosensors responses have not yet been fully understood to study only one these processes. In this work, results studies juglone and bacterial cells receptors are presented. It was shown contribution reactive...
Background: Halogenated aromatic compounds are more resistant to microbial degradation than non-halogenated compounds. Microbial of sodium benzoate in the presence 3-chlorobenzoate is interest. The ability degrade largely determined by substrate specificity first enzyme that initiates degradation, namely, 1,2-dioxygenase for and degradation. In this study, perspective immobilized cells Rhodococcus opacus 1CP actinobacterium was explored. Methods: biosensor approach (a membrane sensor based...