A5056040835- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Image and Object Detection Techniques
- Phase Change Materials Research
- Biomedical Ethics and Regulation
- Pesticide Exposure and Toxicity
- Cancer, Lipids, and Metabolism
- Renal and related cancers
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Engineering Structural Analysis Methods
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Impact of Light on Environment and Health
- Dielectric materials and actuators
- Laser and Thermal Forming Techniques
- Night-time city culture
- Geomagnetism and Paleomagnetism Studies
- Animal Disease Management and Epidemiology
- Scientific Measurement and Uncertainty Evaluation
- Xenotransplantation and immune response
- Refrigeration and Air Conditioning Technologies
- Heat shock proteins research
Shanxi Medical University
2025
Harbin Veterinary Research Institute
2021-2024
Chinese Academy of Agricultural Sciences
2021-2024
Wuyi University
2022-2023
Shandong Institute of Metrology
2020-2023
Liaoning Normal University
2023
Zhejiang Chinese Medical University
2023
Wuyi University
2022
Hebei Medical University
2008-2020
Fourth Hospital of Hebei Medical University
2008-2020
NADC34-like porcine reproductive and respiratory syndrome virus (PRRSV) strains were first detected in China 2017, with epidemic potential. In this study, the phylogenetic, epidemic, recombinant properties of PRRSV evaluated comprehensively. From 2020 to October 2021, 82 isolates obtained from 433 PRRSV-positive clinical samples. These accounted for 11.5% 28.6% positives respectively, have spread eight provinces. We selected 15 samples whole-genome sequencing, revealing genome lengths...
Human beta-defensin 2 (hBD-2) and hBD-3 have potent fungicidal activity in the micromolar range. Although little is known about their mechanism of action against Candida species, some similarities to antifungal salivary peptide histatin 5 (Hst 5) seem exist. Since hBD-2 been reported cause direct disruption target cell membranes, we compared effects on albicans membrane integrity. Incubation calcein-loaded C. cells with a dose lethal for 90% strains tested (LD(90)) resulted maximal dye...
ABSTRACT Histatin 5 (Hst 5) is a salivary cationic peptide that has toxicity for Candida albicans by inducing rapid cellular ion imbalance and cell volume loss. Microarray analyses of peptide-treated cells were used to evaluate global gene responses elicited Hst 5. The major transcriptional response C. was expression genes involved in adaptation osmotic stress, including production glycerol ( RHR2 , SKO1 PDC11 ) the general stress CTA1 HSP70 ). oxidative-stress AHP1 TRX1 GPX1 mildly induced...
Porcine reproductive and respiratory syndrome virus (PRRSV) has brought serious economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years. The prevalence features of on Chinese farms are unclear. We continuously monitored PRRSV a farm with strict biosafety measures Henan Province, China, 2020. results showed that multiple types coexisted this single farm. was one the main circulating strains responsible infections throughout nearly entire epidemic cycle....
Porcine reproductive and respiratory syndrome virus (PRRSV)-1 PRRSV-2 have long been cocirculating in China. To date, all PRRSV-1 strains China classified as subtype 1. We investigated the prevalence of several areas from 2016 to 2022 found that BJEU06-1-like comprised main epidemic branch PRRSV-1. Pathogenicity data for this subgroup are currently lacking. In study, Chinese strain ZD-1 was isolated primary alveolar macrophages (PAMs). has undergone no recombination a 5-aa discontinuous...
Candida albicans Hsp70 Ssa1/2 proteins have been identified as cell wall binding partners for the antifungal cationic peptide Histatin 5 (Hst 5) in vivo. C. Ssa2p plays a major role and translocation of Hst into fungal cells, demonstrated by defective uptake killing SSA2 null mutants. Candidal are classical chaperone with two discrete functional domains consisting ATP regions. Pull-down assays full-length truncated Ssa2 found that ATPase domain was required binding. Further mapping limited...
The newly emerged sublineage 1.5 (NADC34-like) porcine reproductive and respiratory syndrome virus (PRRSV) has posed a direct threat to the Chinese pig industry since 2018. However, prevalence impact of NADC34-like PRRSV on farms is unclear. In present study, we continuously monitored pathogens-including PRRSV, African swine fever (ASFV), classical (CSFV), pseudorabies (PRV), circovirus 2 (PCV2)-on fattening farm with strict biosecurity practices located in Heilongjiang Province, China, from...
Abstract The matrix metalloproteinases ( MMPs ) and tissue inhibitor of TIMPs may contribute to the development adenomyosis. aim present study was investigate whether three single nucleotide polymorphisms (SNPs) in promoter regions MMP‐2 (−1306 C/T −735 TIMP‐2 (‐ 418G/C genes were related risk adenomyosis development. Genotypes determined by polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) assay 180 patients 324 frequency‐matched control women a Chinese...
A latex microsphere-based immunochromatographic strip (ICS) test was successfully developed for the rapid and sensitive detection of porcine reproductive respiratory syndrome virus (PRRSV). The PRRSV N protein-specific monoclonal antibody (mAb) 1H4 labeled with microspheres dispensed on a conjugate pad use as detector. same mAb goat anti-mouse were blotted nitrocellulose membrane to generate control lines, respectively. limit approximately 5 × 102.0 median tissue culture infectious dose...
Porcine reproductive and respiratory syndrome virus (PRRSV) has caused serious economic losses to the pig industry worldwide. During continuous monitoring of PRRSV, a new PRRSV strain type with novel characteristics was first identified in three different regions Shandong Province. These strains presented deletion pattern (1 + 8 1) NSP2 region belonged branch sublineage 8.7 based on ORF5 gene phylogenetic tree. To further study genomic new-branch we selected sample from each farms for...
NADC34-like porcine reproductive and respiratory syndrome virus (PRRSV) strains were first detected in China 2017 became major circulating 2021. Our previous study showed that the live-attenuated vaccine candidate SD-R strain could provide broad cross-protection against different NADC30-like PRRSVs (sublineage 1.8). However, protective effect of PRRSV is unclear. Here, a novel PRRSV, LNTZJ1341-2012, was isolated from pig farm experiencing disease 2020. Sequence analysis revealed...
Both highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) NADC30-like PRRSV have caused tremendous economic losses to the Chinese pig industry. In this study, a good challenge model was established evaluate protection afforded by candidate SD-R vaccine against infection with representative HP-PRRSV strain (HuN4). The control piglets in experiment displayed obvious clinical symptoms of infection, mortality rate up 40%. contrast, all vaccinated challenged group...
One-second resolution geomagnetic data from 5 stations located at low-latitudes (i.e., L = 1.2 ~ 1.6) were used to examine the local time dependence of dominant frequency Pi2 pulsations. We analyzed 183 events simultaneously recorded and discussed their possible generation mechanisms. The averaged H (horizontal) component is higher on dawn side than that dusk shows a peak value in post-midnight around 03 LT, which confirms LT previously suggested single station non-simultaneous observation)....
Since 2011, pseudorabies virus (PRV) has recurred in several vaccinated pig farms China. PRV variants with high virulence were found to be the main cause of outbreaks. In face epidemic, detection wild strain is as important vaccine immunization, so we hoped achieve differential diagnosis by obtaining a monoclonal antibody (mAB) that could used identify strain. this study, novel immunization and screening strategy prepare an mAB obtained 1H5 against gE glycoprotein. An immunofluorescence...
China suffered from severe economic stagnation and urban decay during the COVID-19 pandemic, even public mental health was threatened. To create a favorable consumption environment for enhancing development, meeting needs of life, Chinese government has transformed built series national night cultural tourism agglomeration areas as new type functional area in cities. The present study uses spatial analysis methods such nearest neighbor index, nuclear density analysis, coefficient geographic...
Poly(vinylidene fluoride) (PVDF) membranes were blended with loading different kinds of carboxylated multi-walled carbon nanotubes (MWCNTs) by phase inversion method. PVDF molecular weight 450 and 800 kDa was selected for our study, whereas four CNTs as pristine nanotubes, carboxyl content from 0.49, 2 to 3.86 wt.% chosen a comparison. The casting solution set 18 wt%. effects in MWCNTs on membrane morphology, surface roughness, hydrophilicity, pore size porosity comprehensively investigated...
Image recognition of pointer instrument based on machine vision has been widely used. However, traditional algorithms are inadequate in the case intersectant dual-pointer instrument. This paper provides a complete solution, including image pretreatment, extraction position and reading identification. In particular, target line segment filter method is invented.
Gene-modified miniature pigs serve as alternative tissue and organ donors for xenotransplantation to alleviate the shortage of human allogenic organs. However, high copy number porcine endogenous retrovirus (PERV) genomes integrates with genome, which has a potential risk cross-species transmission hinders clinical practice xenotransplantation. Recently, CRISPR/Cas9 been used inactivate PERVs. Cas9 also triggers severe DNA damage at multiple integrated PERV sites in induces senescence...
Objective To compare the clinical effect of penehyclidine hydrochloride and atropine in treatment acute organophosphorus pesticide poisoning. Methods 86 patients with poisoning were selected randomly assigned to observation group control according digital table, 43 cases each group. The two groups given conventional treatment, while was treated atropine, hydrochloride.The disappearance time main symptoms, rescue success rate adverse reactions observed. Results After salvage...