A5047132545- Genetics, Aging, and Longevity in Model Organisms
- Scientific Computing and Data Management
- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Circadian rhythm and melatonin
- Big Data and Business Intelligence
- Online Learning and Analytics
- Mitochondrial Function and Pathology
- Neuroendocrine regulation and behavior
- Protist diversity and phylogeny
- Online and Blended Learning
- Geographic Information Systems Studies
- Data Visualization and Analytics
- Photosynthetic Processes and Mechanisms
- Algorithms and Data Compression
- Web and Library Services
- Face and Expression Recognition
- Data Analysis with R
- Advanced Statistical Methods and Models
- Cardiomyopathy and Myosin Studies
- Generative Adversarial Networks and Image Synthesis
- Fungal and yeast genetics research
- Statistics Education and Methodologies
- Genetic Syndromes and Imprinting
- Chromosomal and Genetic Variations
Simon Fraser University
2013-2023
University of British Columbia
2008-2022
University of Saskatchewan
2022
Brain (Germany)
2008
Primary cilia are specialised sensory and developmental signalling devices extending from the surface of most eukaryotic cells. Defects in these organelles cause inherited human disorders (ciliopathies) such as retinitis pigmentosa Bardet-Biedl syndrome (BBS), frequently affecting many physiological processes across multiple organs. Cilium formation, maintenance function depend on intracellular transport systems intraflagellar (IFT), which is driven by kinesin-2 IFT-dynein motors regulated...
Primary cilia are microtubule-based sensory organelles whose structures and functions must be actively maintained throughout animal lifespan to support signal transduction pathways essential for development physiological processes such as vision olfaction [1Goetz S.C. Anderson K.V. The primary cilium: a signalling centre during vertebrate development.Nat. Rev. Genet. 2010; 11: 331-344Crossref PubMed Scopus (1342) Google Scholar]. Remarkably, few cellular components aside from the...
We investigated the role of Caenorhabditis elegans CREB (cAMP response element binding protein) homologue, crh-1, in to tap (nonlocalized mechanosensory stimulation) and habituation. Worms with a loss-of-function mutation crh-1 performed smaller reversals than did wild-type worms not show long-term memory for spaced training 24-hr posttraining; however, they short-term habituation stimuli when were presented at both 10-s 60-s interstimulus intervals, showed 12-hr intermediate...
The distinction between non-associative and associative forms of learning has historically been based on the behavioral training paradigm. Through discovering molecular mechanisms that mediate learning, we can develop a deeper understanding relationships different learning. Here, genetically dissect short- long-term memory for form habituation an context conditioning habituation, in nematode Caenorhabditis elegans. In short-term chemosensory worms trained tested presence either taste (sodium...
Habituation is a ubiquitous form of non-associative learning observed as decrement in responding to repeated stimulation that cannot be explained by sensory adaptation or motor fatigue. One the defining characteristics habituation its sensitivity rate at which training stimuli are presented—animals habituate faster response more rapid stimulation. The molecular mechanisms underlying this interstimulus interval (ISI)-dependent characteristic remain unknown. In article, we use behavioural...
Lasting memories are likely to result from a lasting change in neurotransmission. In the nematode Caenorhabditis elegans , spaced training with tap stimulus induces habituation that lasts for >24 h and is dependent on glutamate transmission, postsynaptic AMPA receptors, CREB. Here we describe distinct, presynaptic mechanism shorter memory induced by massed training. We report FMRFamide-related peptide (FMRF = Phe-Met-Arg-Phe-NH 2 ), FLP-20, critical 12 following training, but not required...
Forward genetic screens represent powerful, unbiased approaches to uncover novel components in any biological process. Such suffer from a major bottleneck, however, namely the cloning of corresponding genes causing phenotypic variation. Reverse have been employed as way circumvent this issue, but can often be limited scope. Here we demonstrate an innovative approach gene discovery. Using C. elegans model system, used whole-genome sequenced multi-mutation library, Million Mutation Project,...
Neurons throughout the mammalian brain possess non-motile cilia, organelles with varied functions in sensory physiology and cellular signaling. Yet, roles of cilia these neurons are poorly understood. To shed light into their functions, we studied EFHC1, an evolutionarily conserved protein required for motile function linked to a common form inherited epilepsy humans, juvenile myoclonic (JME). We demonstrate that C. elegans EFHC-1 within specialized mechanosensory where it regulates neuronal...
In the data science courses at University of British Columbia, we define as study, development and practice reproducible auditable processes to obtain insight from data. While reproducibility is core our definition, most learners enter field with other aspects in mind, for example predictive modelling, which often one interesting topic novices. This fact, along highly technical nature industry standard tools currently employed science, present out-of-the gate challenges teaching classroom....
Abstract The ability to learn is an evolutionarily conserved adaptation that remains incompletely understood. Genetically tractable model organisms facilitate mechanistic explanations of learning span genetic, neural circuit, and behavioural levels. Many aspects physiology, including processes underlie ( e.g. neurotransmitter release long-lasting changes in synaptic strength), are regulated by brief local [ μ m] levels free intracellular Ca 2+ . On this scale, [Ca ] activate many -sensors,...
The conserved Shugoshin (SGO) protein family is essential for mediating proper chromosome segregation from yeast to humans but has also been implicated in diverse roles outside of the nucleus. SGO's include inhibiting incorrect spindle attachment kinetochore, regulating assembly checkpoint (SAC), and ensuring centriole cohesion centrosome, all functions that involve different microtubule scaffolding structures cell. In Caenorhabditis elegans, a species with holocentric chromosomes, SGO-1 not...