A5069754990- Genomics and Chromatin Dynamics
- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Acute Lymphoblastic Leukemia research
- Hemoglobinopathies and Related Disorders
- Animal Genetics and Reproduction
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related gene regulation
- DNA and Nucleic Acid Chemistry
- Immune Response and Inflammation
- Chronic Myeloid Leukemia Treatments
- DNA Repair Mechanisms
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Cancer-related Molecular Pathways
- Erythrocyte Function and Pathophysiology
- Chromosomal and Genetic Variations
- Chronic Lymphocytic Leukemia Research
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
Université de Montréal
2012-2024
Centre Intégré Universitaire de Santé et de Services Sociaux du Centre-Sud-de-l'Île-de-Montréal
2019-2024
Centre Intégré Universitaire de Santé et de Services Sociaux du Saguenay–Lac-Saint-Jean
2024
Hôpital Maisonneuve-Rosemont
2010-2023
Institute for Research in Immunology and Cancer
2006-2009
Erasmus University Rotterdam
1996-2007
Jewish General Hospital
2006
Erasmus MC
1996-2001
University of Fukui
2000
Canadian Red Cross Society
1992
The candidate tumor suppressor BAP1 is a deubiquitinating enzyme (DUB) involved in the regulation of cell proliferation, although molecular mechanisms governing its function remain poorly defined. was recently shown to interact with and deubiquitinate transcriptional regulator host factor 1 (HCF-1). Here we show that assembles multiprotein complexes containing numerous transcription factors cofactors, including HCF-1 Yin Yang (YY1). Through coiled-coil motif, directly interacts zinc fingers...
Eukaryotic cells have evolved highly orchestrated protein catabolic machineries responsible for the timely and selective disposal of proteins organelles, thereby ensuring amino acid recycling. However, how degradation is coordinated with supply synthesis has remained largely elusive. Here we show that mammalian proteasome undergoes liquid-liquid phase separation in nucleus upon deprivation. We termed these condensates SIPAN (Starvation-Induced Proteasome Assemblies Nucleus) are a common...
During development and erythropoiesis, globin gene expression is finely modulated through an important network of transcription factors chromatin modifying activities. In this report we provide in vivo evidence that endogenous Ikaros recruited to the human beta-globin locus targets histone deacetylase HDAC1 remodeling protein Mi-2 gamma-gene promoters, thereby contributing gamma-globin silencing at time gamma- transcriptional switch. We show for first interacts with GATA-1 enhances binding...
The transcription factor Hairy Enhancer of Split 1 (HES1), a downstream effector the Notch signaling pathway, is an important regulator hematopoiesis. Here, we demonstrate that in primary erythroid cells, Hes1 gene expression transiently repressed around proerythroblast stage differentiation. Using mouse erythroleukemia found RNA interference (RNAi)-mediated depletion HES1 enhances cell differentiation, suggesting this protein opposes terminal This also supported by decreased differentiation...
IKAROS is a critical regulator of hematopoietic cell fate and its dynamic expression pattern required for proper hematopoiesis. In collaboration with the Nucleosome Remodeling Deacetylase (NuRD) complex, it promotes gene repression activation. It remains to be clarified how can support transcription activation while being associated HDAC-containing complex NuRD. also binds Positive-Transcription Elongation Factor b (P-TEFb) at promoters. Here, we demonstrate that NuRD P-TEFb are assembled in...
Suppressed neutrophil apoptosis, a hallmark of sepsis, perpetuates inflammation and delays resolution. Myeloid nuclear differentiation antigen (MNDA) is expressed only in myeloid cells has been implicated cell differentiation; however, its function mature neutrophils not known.We studied whether MNDA could contribute to regulation apoptosis from healthy subjects patients with investigated the impact knockdown on apoptosis.Human were challenged mediators sepsis cultured investigate cleavage...
Sepsis and septic shock are characterized by prolonged inflammation delayed resolution, which associated with suppression of neutrophil apoptosis. The role the intrinsic apoptotic pathway intracellular factors in regulation apoptosis remain incompletely understood. We previously reported that nuclear factor MNDA (myeloid differentiation antigen) is fundamental to execution constitutive death program. During cleaved caspases relocated cytoplasm. However, when challenged known mediators...
Ikaros is associated with both gene transcriptional activation and repression in lymphocytes. acts also as repressor of human γ-globin (huγ-) transcription fetal adult erythroid cells. Whether eventually, how can function a activator cells remains poorly understood. Results presented herein demonstrate that developmental-specific huγ-gene expression yolk sac Molecular analysis primary revealed interacts Gata-1 favors Brg1 recruitment to the β-globin Locus Control Region huγ-promoters,...
Inflammation control is critical during the innate immune response. Such response triggered by detection of molecules originating from pathogens or damaged host cells pattern-recognition receptors (PRRs). PRRs subsequently initiate intra-cellular signalling through different pathways, resulting in i) production inflammatory cytokines, including type I interferon (IFN), and ii) initiation a cascade events that promote both immediate responses as well adaptive responses. All human PYRIN...