A5066291977- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- TGF-β signaling in diseases
- Signaling Pathways in Disease
- Platelet Disorders and Treatments
- Carcinogens and Genotoxicity Assessment
- Venomous Animal Envenomation and Studies
- Natural product bioactivities and synthesis
- Invertebrate Immune Response Mechanisms
- Drug Transport and Resistance Mechanisms
- Antimicrobial Peptides and Activities
- Kruppel-like factors research
- DNA and Nucleic Acid Chemistry
- Phytochemistry and Biological Activities
- DNA Repair Mechanisms
- Fluid Dynamics and Turbulent Flows
- Growth Hormone and Insulin-like Growth Factors
- Metabolism, Diabetes, and Cancer
- Peptidase Inhibition and Analysis
- Chemical Synthesis and Analysis
- Advanced Thermoelectric Materials and Devices
- Synthetic Organic Chemistry Methods
- Cavitation Phenomena in Pumps
- Bone Metabolism and Diseases
Kagawa University
2013-2025
University of Fukui
2019-2024
Toshiba (Japan)
1993-2024
The University of Tokyo
2005-2024
Kyoto Pharmaceutical University
2018-2023
Himeji Dokkyo University
2008-2022
Murata (Japan)
1995-2020
Nissan Chemical Corporation (Japan)
2003-2019
Engineering (Italy)
2019
Faculty (United Kingdom)
2019
Activin, a member of the transforming growth factor beta protein family, was originally isolated from gonadal fluids and stimulates release pituitary follicle-stimulating hormone (FSH). Activin has numerous functions in both normal neoplastic cells. Various cells synthesize activin have specific binding site for this peptide. However, molecular basis its actions is unknown. A purified rat ovary identical to follistatin, inhibitor FSH release. It likely that participates diverse regulatory activin.
A growth factor (HGF) stimulating DNA synthesis of adult rat hepatocytes in primary culture was found platelets. HGF purified from platelets to homogeneity by a three-step procedure: stimulation its release thrombin, cation-exchanger fast protein liquid chromatography on Mono S column, and heparin-Sepharose chromatography. clearly distinguishable the platelet-derived (PDGF) heat- acid-labile cationic that inactivated reduction with dithiothreitol. Its molecular mass estimated be 27 kDa...
A cationic peptide, designated tachyplesin, was isolated from acid extracts of horseshoe crab (Tachypleus tridentatus) hemocyte debris. It consists 17 residues and the structure determined by Edman degradation is: (formula; see text) The carboxyl-terminal end this peptide identified as arginine alpha-amide, whole sequence including alpha-amide also confirmed fast atom bombardment mass spectrometry, indicating a value 2263. Tachyplesin inhibits growth both Gram-negative -positive bacteria at...
It is unclear whether organ-specific autoantigens are critical for the development of primary Sjögren's syndrome (SS). A 120-kilodalton autoantigen was purified from salivary gland tissues an NFS/sld mouse model human SS. The amino-terminal residues were identical to those cytoskeletal protein alpha-fodrin. antigen induced proliferative T cell responses and production interleukin-2 interferon-gamma in vitro. Neonatal immunization with prevented disease mice. Sera patients SS reacted...
The primary structure of rat hepatocyte growth factor (HGF) was elucidated by determining the base sequence complementary DNA (cDNA) HGF. cDNA for HGF isolated screening a liver library with oligonucleotides based on partial N-terminal amino acid beta subunit purified is encoded in an mRNA about 6 kilobases. Both alpha and subunits are specified single open reading frame 728-amino protein calculated molecular weight 82,904. part has signal prosequence 30 25 residues, respectively. mature...
Abstract Galectin-9 (Gal-9) induced the apoptosis of not only T cell lines but also other types in a dose- and time-dependent manner. The was suppressed by lactose, sucrose, indicating that β-galactoside binding is essential for Gal-9-induced apoptosis. Moreover, Gal-9 required at least 60 min possibly de novo protein synthesis to mediate We assessed peripheral blood cells Gal-9. Apoptosis both activated CD4+ CD8+ cells, former were more susceptible than latter. A pan-caspase inhibitor...
Members of the ATP-binding cassette (ABC) transporter superfamily are mutated to cause diseases that include cystic fibrosis, hyperinsulinemia, adrenoleukodys-trophy, Stargardt disease and multidrug resistance. We recently isolated a novel human member ABC as candidate for glucuronide glutathione-conjugated antitumor agents, found it highly homologous rat cmoat gene. Consistent with recent findings defects in gene two models hyperbilirubinemia (TR− Eisai), we report deletions missense...
Hepatocyte growth factor (HGF) is a potent mitogen for primary hepatocytes. Therefore, we examined HGF as possible autocrine in hepatocellular carcinoma (HCC). We introduced an albumin-HGF expression vector into Fao HCC cells and transgenic mice. Expression of the inhibited their vitro. In vivo, FaoHGF produced tumors that averaged 10% sizes G418-resistant controls when transplanted nude contrast, hepatocytes from mice expressing grew more rapidly than did those normal siblings. Further,...
Follistatin (FS), an activin-binding protein, is a monomer derived from two polypeptide core sequences of 315 (FS-315) and 288 (FS-288) amino acids originated alternatively spliced mRNA. To define the structural heterogeneity native FS, we purified six molecular forms FS porcine ovaries. Protein chemical analysis revealed that differences among isoforms were caused by truncation carboxyl-terminal region and/or presence carbohydrate chains, resulting in formation FS-315, FS-288, composed 303...
A binding protein for activin was purified from bovine pituitary by affinity chromatography on dextran sulfate- Sepharose CL-4B and activin-Affi-Gel 10. 52,700-fold purification over the starting crude homogenate achieved. The preparation showed two bands of 36 33 kilodalton in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under nonreducing conditions. ability each form to specifically bind determined ligand blot analysis competition study. Each found have same NH2-terminus its...
Abstract Maturation of dendritic cells (DCs) is critical for initiation immune responses and regulated by various stimulatory signals. We assessed the role galectin (Gal)-9 in DC maturation. Culture immature DCs with exogenous Gal-9 markedly increased surface expression CD40, CD54, CD80, CD83, CD86, HLA-DR a dose-dependent manner, although had no or little effect on differentiation human monocytes into DCs. Gal-9-treated secreted IL-12 but not IL-10, they elicited production Th1 cytokines...
Abstract Purpose: Galectin-9, a member of the β-galactoside–binding galectin family, induces aggregation certain cell types. We assessed contribution galectin-9 to breast cancer cells as well relation between expression in tumor tissue and distant metastasis patients with cancer. Experimental Design: Subclones MCF-7 high or low levels were established either cultured on plastic dishes transplanted into nude mice. The tumors 84 tested for by immunohistochemistry. followed up 14 years....
Abstract Galectin‐9 expression was examined in 6 human melanoma cell lines. Among them, MM‐BP proliferated with colony formation, but MM‐RU failed. RT‐PCR analysis revealed evident of galectin‐9 mRNA not MM‐RU. expressed protein both on the surface and cytoplasm, whereas it only weakly cytoplasm. Exogenous induced vitro aggregation apoptosis proliferating without formation. Association cells prognosis patients bearing melanocytic tumors further examined. strongly homogeneously nevi,...
Factor C is an endotoxin-sensitive, intracellular serine protease zymogen which initiates the coagulation cascade system in limulus hemolymph. We have determined entire amino acid sequence of factor using recombinant DNA technique. The consisted 994 residues with a calculated molecular mass 109,648 Da. Most interestingly, has five repeating units (Sushi domain or short consensus repeat) about 60 each, been found many proteins participating mammalian complement system. In addition to typical...
Follistatin, an activin-binding protein secreted by cultured rat granulosa cells, was shown to associate with the cell surface affinity labeling 125I-activin. Addition of follistatin cells demonstrated a typical ligand-binding saturation curve, suggesting that have specific binding site for follistatin. This markedly inhibited heparin and heparan sulfate, but not chondroitin sulfates A C, keratan dermatan sulfate. When were treated glycosaminoglycan-degrading enzymes before or after addition...
Exposure of limulus hemocytes to bacterial endotoxins (lipopolysaccharide, LPS) results in the activation intracellular clotting system, consisting several protein components. During separation these components, a potent anticoagulant, named anti-LPS factor, which inhibits endotoxin-mediated coagulation cascade, was found from both Tachypleus tridentatus and Limulus polyphemus (Tanaka, S., et al. (1982) Biochem. Biophys. Res. Commun. 105, 717–723). The principle isolated hemocyte lysate by...
A potent anticoagulant, anti-lipopolysaccharide (LPS) factor, found in limulus hemocytes