A5078315361- SARS-CoV-2 and COVID-19 Research
- Genetic factors in colorectal cancer
- COVID-19 Clinical Research Studies
- Colorectal Cancer Treatments and Studies
- Long-Term Effects of COVID-19
- Epigenetics and DNA Methylation
- Mosquito-borne diseases and control
- Gastric Cancer Management and Outcomes
- RNA modifications and cancer
- Viral Infections and Vectors
- Genetic Associations and Epidemiology
- Cancer-related gene regulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Virology and Viral Diseases
- Peptidase Inhibition and Analysis
- Cancer Genomics and Diagnostics
- Protein Degradation and Inhibitors
- Melanoma and MAPK Pathways
- Autism Spectrum Disorder Research
- Cutaneous Melanoma Detection and Management
- Microplastics and Plastic Pollution
- interferon and immune responses
- Viral Infections and Outbreaks Research
- Bipolar Disorder and Treatment
QIMR Berghofer Medical Research Institute
2016-2025
Royal Brisbane and Women's Hospital
2014
// Catherine E. Bond 1 , Diane M. McKeone Murugan Kalimutho 2 Mark L. Bettington 1, 3, 4 Sally-Ann Pearson Troy D. Dumenil Leesa F. Wockner 5 Matthew Burge 6 Barbara A. Leggett 4, 7 Vicki L.J. Whitehall 8 Conjoint Gastroenterology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Queensland, Australia Signal Transduction 3 Envoi Specialist Pathologists, School of Medicine, University Cancer and Population Studies, Department Oncology, Royal Brisbane Women's Hospital,...
Global microplastic (MP) contamination and the effects on environment are well described. However, potential for MP consumption to affect human health remains controversial. Mice consuming ≈80 μg/kg/day of 1 μm polystyrene MPs via their drinking water showed no weight loss, nor were detected in internal organs. The microbiome was also not significantly changed. did lead small transcriptional changes colon suggesting plasma membrane perturbations mild inflammation. challenged with...
The reduced pathogenicity of the omicron BA.1 sub-lineage compared to earlier variants is well described, although whether such attenuation retained for later like BA.5 and XBB remains controversial. We show that isolates were significantly more pathogenic in K18-hACE2 mice than a isolate, showing increased neurotropic potential, resulting fulminant brain infection mortality, similar seen original ancestral isolates. also infected human cortical organoids greater extent In brains mice,...
Human
Colorectal cancer is a major cause of death and approximately 20% arises within serrated polyps, which are under-recognized poorly understood. Human colorectal polyps frequently exhibit both oncogenic BRAF mutation widespread DNA methylation changes, important in silencing genes restraining neoplastic progression. Here, we investigated whether vivo induction mutant Braf sufficient to result coordinated promoter changes for multiple cancer-related genes. The BrafV637E was induced murine...
Background & AimsColorectal cancer is an epigenetically heterogeneous disease, however, the extent and spectrum of CpG island methylator phenotype (CIMP) not clear.MethodsGenome-scale methylation transcript expression were measured by DNA Methylation RNA microarray in 216 unselected colorectal cancers, findings validated using The Cancer Genome Atlas 450K sequencing data. Mutations epigenetic regulators assessed CIMP-subtyped exomes.ResultsCIMP-high cancers dichotomized into CIMP-H1 CIMP-H2...
SARS-CoV-2 uses the human ACE2 (hACE2) receptor for cell attachment and entry, with mouse (mACE2) unable to support infection. Herein we describe an ACE2-lentivirus system illustrate its utility in vitro vivo infection models. Transduction of non-permissive lines hACE2 imparted replication competence, transduction mACE2 containing N30D, N31K, F83Y H353K substitutions, match hACE2, rescued replication. Intrapulmonary hACE2-lentivirus C57BL/6J mice permitted significant virus lung epithelium....
How well mouse models recapitulate the transcriptional profiles seen in humans remains debatable, with both conservation and diversity identified various settings. Herein we use RNA-Seq data bioinformatics approaches to analyze responses SARS-CoV-2 infected lungs, comparing 4 human studies widely used K18-hACE2 model, a model where hACE2 is expressed from ACE2 promoter, that uses adapted virus wild-type mice. Overlap of single copy orthologue differentially genes (scoDEGs) between was...
Granzyme A (GZMA) is a serine protease secreted by cytotoxic lymphocytes, with Gzma -/- mouse studies having informed our understanding of GZMA’s physiological function. We show herein that mice have mixed C57BL/6J and C57BL/6N genetic background retain the full-length nicotinamide nucleotide transhydrogenase ( Nnt ) gene, whereas truncated in mice. Chikungunya viral arthritis was substantially ameliorated mice; however, presence background, rather than loss GZMA expression, responsible for...
Self-amplifying mRNA (SAM) vaccines can be rapidly deployed in the event of disease outbreaks. A legitimate safety concern is potential for recombination between alphavirus-based SAM and circulating viruses. This theoretical risk needs to assessed regulatory process vaccine approval. Herein, we undertake extensive vitro vivo assessments explore a wide selection alphaviruses coronavirus. were found effectively limit alphavirus co-infection through superinfection exclusion, although some...
Abstract The Val158Met polymorphism of the gene encoding catechol‐ O ‐methyltransferase (COMT) is one most widely tested variants for association with psychiatric disorders, but replication has been inconsistent including both sex limitation and heterogeneity associated allele. In this study we investigate between three SNPs from COMT anxiety depression disorders neuroticism all measured within same sample. Participants were selected as sibling pairs (or multiples) that either concordant or...
Formalin fixation and embedding of clinical tissue samples in paraffin is a common method for archiving biological material. These are often well annotated provide an invaluable resource research. However, this process storage leads to DNA damage fragmentation. The use from formalin fixed, paraffin‐embedded (FFPE) interrogate methylation levels on genome‐wide scale can pose challenges. We compared fresh matched FFPE using the Illumina Infinium HD Human Methylation 450K BeadChip platform with...
WNT activation is a hallmark of colorectal cancer. BRAF mutation present in 15% cancers, and the role mutations signaling regulators this context unclear. Here, we evaluate mutational landscape mutant cancers.we performed exome-sequencing on 24 cancers analyzed these data combination with 175 publicly available cancer exomes. We assessed somatic regulators, hotspot driver analyses to identify potential drivers signaling. The effects Apc Braf were modelled, vivo, using Apcmin/+...
Angiotensin-converting enzyme 2 (ACE2) is the primary entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV-2), but ACE2-independent has been observed in vitro strains with spike-E484D substitution. Here, we conduct a whole-genome CRISPR-Cas9 knockout screen using SARS-CoV-2 mouse adapted 1 (SARS-CoV-2
Warmer climatic conditions have been associated with fewer COVID-19 cases. Herein we infected K18-hACE2 mice housed at the standard animal house temperature of ∼22 °C, or ∼31 which is considered to be thermoneutral for mice. On day 2 post infection, RNA-Seq analyses showed no significant differential gene expression lung in lungs two temperatures, almost identical viral loads and type I interferon responses. There was also difference on 5, but histology clearly elevated inflammatory...
Mayaro virus (MAYV) is associated with acute rheumatic disease that can be debilitating and evolve into months of chronic arthralgia. MAYV believed to have the potential emerge as a tropical public health threat, especially if it develops ability efficiently transmitted by urban mosquito vectors, such Aedes aegypti and/or albopictus .
Abstract N-linked glycosylation of flavivirus envelope proteins is widely viewed as being required for optimal folding, processing and/or transit proteins, and the assembling virons, through endoplasmic reticulum (ER) Golgi. Zika virus (ZIKV) has a single glycan located adjacent to fusion loop. Herein we show that independent serial passage ZIKVNatal in Rag1-/- mice 223 or 386 days, generated two unique glycan-deficient mutants, ZIKV-V153D ZIKV-N154D, respectively. Surprisingly, these...
Getah virus (GETV) is a mosquito-transmitted alphavirus primarily associated with disease in horses and pigs Asia. GETV was also reported to have been isolated from mosquitoes Australia 1961; however, retrieval sequencing of the original isolates (N544 N554), illustrated that these viruses were virtually identical 1955 GETVMM2021 isolate Malaysia. K-mer mining >40,000 terabases sequence data Sequence Read Archive followed by BLASTn confirmation identified multiple sequences biosamples Asia...
Functional assays that assess mRNA splicing can be used in interpretation of the clinical significance sequence variants, including Lynch syndrome associated mismatch repair (MMR) genes. The purpose this study was to investigate contribution assay data classification MMR gene variants. We assayed for 24 variants MLH1, MSH2, and MSH6, 12 missense were also assessed using a cell-free vitro activity (CIMRA) assay. Multifactorial likelihood analysis conducted each variant, combining CIMRA...
Poxvirus systems have been extensively used as vaccine vectors. Herein a RNA-Seq analysis of intramuscular injection sites provided detailed insights into host innate immune responses, well expression vector and recombinant immunogen genes, after vaccination with new multiplication defective, vaccinia-based vector, Sementis Copenhagen Vector. Chikungunya Zika virus mRNA protein was associated necrosing skeletal muscle cells surrounded by mixed cellular infiltrates. The multiple adjuvant...