A5089859174- Mosquito-borne diseases and control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Viral Infections and Outbreaks Research
- Insect symbiosis and bacterial influences
- Microplastics and Plastic Pollution
- Virology and Viral Diseases
- Connective Tissue Growth Factor Research
- Nanoparticles: synthesis and applications
- Dermatologic Treatments and Research
- Insect Resistance and Genetics
- Plant Virus Research Studies
- interferon and immune responses
- Nutrition and Health in Aging
- HIV Research and Treatment
- Phagocytosis and Immune Regulation
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Diet and metabolism studies
- Iron Metabolism and Disorders
- Hedgehog Signaling Pathway Studies
- Peroxisome Proliferator-Activated Receptors
- Genetic and Kidney Cyst Diseases
QIMR Berghofer Medical Research Institute
2016-2025
Bioscience (China)
2025
First Affiliated Hospital of GuangXi Medical University
2024
Guangxi Medical University
2024
Wuhan University
2015
Green Technology
2012
Qingdao Agricultural University
2010
Chinese Academy of Tropical Agricultural Sciences
2010
Hunan City University
2009
Shihezi University
2007-2008
Chikungunya virus (CHIKV) is an arthritogenic alphavirus causing epidemics of acute and chronic arthritic disease. Herein we describe a comprehensive RNA-Seq analysis feet lymph nodes at peak viraemia (day 2 post infection), arthritis 7) disease 30) in the CHIKV adult wild-type mouse model. Genes previously shown to be up-regulated patients were also sequence information was obtained with up ≈8% reads mapping viral genome; however, no adaptive genome changes apparent. Although day 2, 7 30...
Insect-specific Binjari virus facilitates the production of chimeric particles flavivirus pathogens for diagnostics and vaccines.
Abstract Zika and chikungunya viruses have caused major epidemics are transmitted by Aedes aegypti and/or albopictu s mosquitoes. The “Sementis Copenhagen Vector” (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe single-vector construct SCV encodes the structural polyprotein cassettes of both from different loci. A single vaccination mice induces neutralizing antibodies to...
Global microplastic (MP) contamination and the effects on environment are well described. However, potential for MP consumption to affect human health remains controversial. Mice consuming ≈80 μg/kg/day of 1 μm polystyrene MPs via their drinking water showed no weight loss, nor were detected in internal organs. The microbiome was also not significantly changed. did lead small transcriptional changes colon suggesting plasma membrane perturbations mild inflammation. challenged with...
The reduced pathogenicity of the omicron BA.1 sub-lineage compared to earlier variants is well described, although whether such attenuation retained for later like BA.5 and XBB remains controversial. We show that isolates were significantly more pathogenic in K18-hACE2 mice than a isolate, showing increased neurotropic potential, resulting fulminant brain infection mortality, similar seen original ancestral isolates. also infected human cortical organoids greater extent In brains mice,...
Abstract Human infections with the Japanese encephalitis virus (JEV) are a leading cause of viral encephalitis. An unprecedented outbreak JEV genotype 4 was recently reported in Australia, an isolate (JEV NSW/22 ) obtained from stillborn piglet brain. Herein we conduct thorough characterization three different mouse strains and human cortical brain organoids (hBOs), determined ability to be neutralized by sera humans vaccinated IMOJEV. less virulent than FU (genotype 2) Nakayama 3) C57BL/6J...
Introduction Global microplastic (MP) pollution is now well recognized, with humans and animals consuming inhaling MPs on a daily basis, growing body of concern surrounding the potential impacts human health. Methods Using mouse model mild COVID-19, we describe herein effects azide-free 1 μm polystyrene MP beads, co-delivered into lungs SARS-CoV-2 omicron BA.5 inoculum. The effect host response to infection was analysed using histopathology RNA-Seq at 2 6 days post-infection (dpi). Results...
Human
Abstract In 2022, a genotype IV (GIV) strain of Japanese encephalitis virus (JEV) caused an unprecedented and widespread outbreak disease in pigs humans Australia. As no veterinary vaccines against JEV are approved Australia all current human derived from (G) III strains, we used the recently described insect-specific Binjari (BinJV) chimeric flavivirus vaccine technology to produce GIV candidate. Herein describe production displaying structural prM E proteins isolate obtained stillborn...
Self-amplifying mRNA (SAM) vaccines can be rapidly deployed in the event of disease outbreaks. A legitimate safety concern is potential for recombination between alphavirus-based SAM and circulating viruses. This theoretical risk needs to assessed regulatory process vaccine approval. Herein, we undertake extensive vitro vivo assessments explore a wide selection alphaviruses coronavirus. were found effectively limit alphavirus co-infection through superinfection exclusion, although some...
Chikungunya virus (CHIKV) belongs to a group of mosquito-borne alphaviruses associated with acute and chronic arthropathy, peripheral limb joints most commonly affected. Using mouse model CHIKV infection arthritic disease, we show that replication the ensuing foot arthropathy were dramatically reduced when mice housed at 30°C, rather than conventional 22°C. The effect was not detectable fever, but dependent on type I interferon responses. Bioinformatics analyses RNA-Seq data after injection...
Chikungunya virus (CHIKV), Ross River (RRV), o'nyong nyong (ONNV), Mayaro (MAYV) and Getah (GETV) represent arthritogenic alphaviruses belonging to the Semliki Forest antigenic complex. Antibodies raised against one of these viruses can cross-react with other serogroup members, suggesting that, for instance, a CHIKV vaccine (deemed commercially viable) might provide cross-protection antigenically related alphaviruses. Herein we use human alphavirus isolates (including new RRV isolate)...
SARS-CoV-2 uses the human ACE2 (hACE2) receptor for cell attachment and entry, with mouse (mACE2) unable to support infection. Herein we describe an ACE2-lentivirus system illustrate its utility in vitro vivo infection models. Transduction of non-permissive lines hACE2 imparted replication competence, transduction mACE2 containing N30D, N31K, F83Y H353K substitutions, match hACE2, rescued replication. Intrapulmonary hACE2-lentivirus C57BL/6J mice permitted significant virus lung epithelium....
How well mouse models recapitulate the transcriptional profiles seen in humans remains debatable, with both conservation and diversity identified various settings. Herein we use RNA-Seq data bioinformatics approaches to analyze responses SARS-CoV-2 infected lungs, comparing 4 human studies widely used K18-hACE2 model, a model where hACE2 is expressed from ACE2 promoter, that uses adapted virus wild-type mice. Overlap of single copy orthologue differentially genes (scoDEGs) between was...
Despite the availability of an effective, live attenuated yellow fever virus (YFV) vaccine (YFV 17D), this flavivirus still causes up to ≈60,000 deaths annually. A number new approaches are seeking address supply issues and improve safety for immunocompromised recipients. Herein we describe adult female IFNAR-/- mouse model YFV 17D infection disease that recapitulates many features in humans. We used evaluate a is based on recently described chimeric Binjari (BinJV) technology. BinJV...
We recently developed a chimeric flavivirus vaccine technology based on the novel insect-specific Binjari virus (BinJV) and used this to generate ZIKV (BinJ/ZIKA-prME) that protected IFNAR
Angiotensin-converting enzyme 2 (ACE2) is the primary entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV-2), but ACE2-independent has been observed in vitro strains with spike-E484D substitution. Here, we conduct a whole-genome CRISPR-Cas9 knockout screen using SARS-CoV-2 mouse adapted 1 (SARS-CoV-2
Chikungunya virus (CHIKV) is a mosquito transmitted alphavirus associated with robust systemic infection and an acute inflammatory rheumatic disease. A high fiber diet has been widely promoted for its ability to ameliorate diseases. Fiber fermented in the gut into short chain fatty acids such as acetate, propionate butyrate, which enter circulation providing anti-inflammatory activities. Herein we show that mice fed clear worsening of CHIKV arthropathy, increased edema neutrophil...
Most vertebrate RNA viruses show pervasive suppression of CpG and UpA dinucleotides, closely resembling the dinucleotide composition host cell transcriptomes. In contrast, is absent in both invertebrate mRNA that exclusively infect arthropods. Arthropod-borne (arbo) are transmitted between hosts by vectors thus encounter potentially conflicting evolutionary pressures different cytoplasmic environments. Using a newly developed Zika virus (ZIKV) model, we have investigated how demands for...
Zika virus (ZIKV) strains are classified into the African and Asian genotypes. The higher virulence of MR766 strain, which has been used extensively in ZIKV research, adult IFNα/β receptor knockout (IFNAR -/- ) mice is widely viewed as an artifact associated with mouse adaptation due to at least 146 passages wild-type suckling brains. To gain insights molecular determinants MR766’s virulence, a series genes from were swapped those genotype PRVABC59 isolate, less virulent IFNAR mice. causes...
Warmer climatic conditions have been associated with fewer COVID-19 cases. Herein we infected K18-hACE2 mice housed at the standard animal house temperature of ∼22 °C, or ∼31 which is considered to be thermoneutral for mice. On day 2 post infection, RNA-Seq analyses showed no significant differential gene expression lung in lungs two temperatures, almost identical viral loads and type I interferon responses. There was also difference on 5, but histology clearly elevated inflammatory...
More than 100 arboviruses, almost all of which have an RNA genome, cause disease in humans. viruses are causing unprecedented health system challenges worldwide, many with little or no specific therapies vaccines available. Certain species mosquito can carry dengue virus (DENV), Zika (ZIKV) and yellow fever (YFV), where co-infection these has occurred. Here, we found that purified synthetic defective interfering particles (DIPs) derived from DENV type 2 (DENV-2) strongly suppressed...
Mayaro virus (MAYV) is associated with acute rheumatic disease that can be debilitating and evolve into months of chronic arthralgia. MAYV believed to have the potential emerge as a tropical public health threat, especially if it develops ability efficiently transmitted by urban mosquito vectors, such Aedes aegypti and/or albopictus .
Zika virus (ZIKV) is the etiological agent of congenital syndrome (CZS), a spectrum birth defects that can lead to life-long disabilities. A range vaccines are in development with target population including pregnant women and child-bearing age. Using recently described chimeric flavivirus vaccine technology based on novel insect-specific Binjari (BinJV), we generated ZIKV (BinJ/ZIKA-prME) illustrate herein its ability protect against fetal brain infection. Female IFNAR−/− mice were...