A5003724521- Bone Tissue Engineering Materials
- TGF-β signaling in diseases
- Orthopaedic implants and arthroplasty
- Spine and Intervertebral Disc Pathology
- Periodontal Regeneration and Treatments
- Bone and Dental Protein Studies
- Dental Implant Techniques and Outcomes
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Bone fractures and treatments
- Protease and Inhibitor Mechanisms
- Connective Tissue Growth Factor Research
- Bone health and treatments
- Diet and metabolism studies
- Oral and Maxillofacial Pathology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Connective tissue disorders research
- Dental materials and restorations
- Mesenchymal stem cell research
- dental development and anomalies
- Medical Imaging and Analysis
- Cell Adhesion Molecules Research
- Renin-Angiotensin System Studies
- Vitamin D Research Studies
- Metabolism and Genetic Disorders
University of Zagreb
2014-2023
Bone morphogenetic proteins (BMPs) participate in organ regeneration through autocrine and paracrine actions, but the existence effects of these systemic circulation is unknown. Using liquid chromatography–mass spectrometry, we identified BMP6, GDF15, BMP1–3 isoform Bmp1 gene plasma samples from healthy volunteers patients with CKD. We isolated endogenous protein demonstrated that it circulates as an active enzyme, evidenced by its ability to cleave dentin matrix protein-1 vitro. In rats...
In the present study, we evaluated an autologous bone graft substitute (ABGS) composed of recombinant human BMP6 (rhBMP6) dispersed within blood coagulum (ABC) used as a physiological carrier for new formation in spine fusion sheep models. The application ABGS included cervical cage use anterior lumbar interbody (ALIF), while posterolateral (PLF) model allograft devitalized particles (ALLO) were applied with and without instrumentation. ALIF model, (rhBMP6/ABC/cage) implants fused...
BMP2 and BMP7, which use bovine Achilles tendon-derived absorbable collagen sponge bone as scaffold, respectively, have been approved graft substitutes for orthopedic dental indications. Here, we describe an osteoinductive autologous substitute (ABGS) that contains recombinant human BMP6 (rhBMP6) dispersed within blood coagulum (ABC) scaffold. The ABGS is created injectable or implantable gel with rhBMP6 binding tightly to plasma proteins fibrin meshwork, examined by dot-blot assays,...
Reduced peripheral serotonin (5HT) in mice lacking tryptophan hydroxylase (TPH1), the rate limiting enzyme for 5HT synthesis, was reported to be anabolic skeleton. However, other studies TPH1 deletion either had no bone effect or an age dependent inhibition of osteoclastic resorption. The role therefore remains poorly understood. To address this issue, we used selective breeding create rat sublines with constitutively high (high-5HT) and low (low-5HT) platelet level (PSL) uptake (PSU)....
In the present study, we describe autologous blood coagulum (ABC) as a physiological carrier for BMP6 to induce new bone formation. Recombinant human (rhBMP6), dispersed within ABC and formed an graft substitute (ABGS), was evaluated either with or without allograft particles (ALLO) in rat subcutaneous implants posterolateral lumbar fusion (PLF) model rabbits. ABGS induced endochondral differentiation implants. Coating ALLO by significantly decreased formation of multinucleated foreign body...
Abstract Aim Regenerative periodontal therapy is often unpredictable and limited. Cementum regeneration necessary for the proper repair of a ligament. The precise mechanism how bone morphogenetic protein‐7 ( BMP 7) induces differentiation mineralization cementoblasts remains undetermined. purpose this study was to evaluate effect 7 on early proteome gene expression profile cementoblastic OCCM .30 cells in vitro. Materials Methods Immortalized murine .30) were exposed evaluated for: (1)...
Abstract Osteocytes are the main cells of bone tissue and play a crucial role in formation resorption. Recent studies have indicated that Diabetes Mellitus (DM) affects mass potentially causes higher fracture risk. Previous work on osteocyte cell cultures has demonstrated mechanotransduction is impaired after culture under diabetic pre-conditioning with high glucose (HG), specifically osteoclast recruitment differentiation. The aim this study was to analyze extracellular metabolic changes...
Posterolateral lumbar fusion (PLF) is a commonly performed surgical procedure for the treatment of pathological conditions lumbosacral spine. In present study, we evaluated an autologous bone graft substitute (ABGS) containing rhBMP6 in blood coagulum (ABC) and synthetic ceramics used as compression resistant matrix (CRM) rabbit PLF model. pilot experiment, tested four different CRMs (BCP 500-1700 μm, BCP 1700-2500 μm two TCP form slabs) which were selected based on achieving uniform ABC...
AimTo explore the possibility of brain imaging by microcomputed tomography (microCT) using x-ray contrasting methods to visualize mouse ischemic lesions after middle cerebral artery occlusion (MCAO).MethodsIsolated brains were immersed in ionic or nonionic radio contrast agent (RCA) for 5 days and subsequently scanned microCT scanner. To verify whether ex-vivo images can be used characterize lesions, they compared Nissl stained serial histological sections same brains. if RCA may afterwards...
Liver fibrosis is a progressive pathological process resulting in an accumulation of excess extracellular matrix proteins. We discovered that bone morphogenetic protein 1-3 (BMP1-3), isoform the metalloproteinase Bmp1 gene, circulates plasma healthy volunteers and its neutralization decreases progression chronic kidney disease 5/6 nephrectomized rats. Here, we investigated potential role BMP1-3 liver disease. Rats with carbon tetrachloride (CCl4)-induced were treated monoclonal anti-BMP1-3...
Bone morphogenetic proteins (BMPs) are potent osteoinductive agents for bone tissue engineering. In order to define optimal properties of a novel autologous graft substitute (ABGS) containing rhBMP6 within the blood coagulum (ABC) and ceramic particles as compression resistant matrix (CRM), we explored influence their amount, chemical composition particle size on quantity quality formation in rat subcutaneous assay. Tested included tricalcium phosphate (TCP), hydroxyapatite (HA) biphasic...
Bone morphogenetic proteins (BMPs) have a major role in tissue development. BMP3 is synthesized osteocytes and mature osteoblasts has an antagonistic effect on other BMPs bone tissue. The main aim of this study was to fully characterize cortical trabecular long bones both male female Bmp3−/− mice. To investigate the Bmp3 from birth maturity, we compared mice with wild-type littermates at following stages postnatal development: 1 day (P0), 2 weeks (P14), 8 16 age. deletion confirmed using...
Abstract Autologous bone graft substitute (ABGS) containing rhBMP6 in autologous blood coagulum (Osteogrow) is a novel therapeutic solution for regeneration. This study aimed to investigate the long-term outcome of ABGS with synthetic ceramics (Osteogrow-C) rabbit posterolateral spinal fusion (PLF) model. Osteogrow-C implants were implanted bilaterally between lumbar transverse processes. We compared following implantation ceramic particles different chemical composition (TCP and biphasic...
Type III transforming growth factor (TGFβ) receptor (TGFβrIII) modulates TGFβ superfamily signaling. Its tumor tissue expression is downregulated in human breast cancer. We determined (indirect ELISA) plasma levels of the soluble (sTGFβrIII) 47 women with cancer (AJCC stages 0-IIB) (cases) pre-surgery and over two months after surgery, 36 healthy (controls). Plasma sTBFβrIII was lower cases than controls (age-adjusted difference -29.7 ng/mL, p < 0.001), discriminated between disease health...
Mushroom extracts have shown promising effects in the treatment of cancer and various chronic diseases. Osteoporosis is considered one most widespread diseases, for which currently available therapies show mixed results. In this research we investigated vitro water culinary-medicinal mushrooms Trametes versicolor, Grifola frondosa, Lentinus edodes, Pleurotus ostreatus on a MC3T3-E1 mouse osteoblast-like cell line, primary rat osteoblasts, osteoclasts. an animal osteoporosis model, rats were...
Individuals with inflammatory bowel disease (IBD) have an increased risk of bone impairment, which is a process controlled by the RANKL/RANK/OPG system, mostly due to chronic inflammation and corticosteroid treatment. Bone morphogenic protein 7 (BMP7) has complex role in maintaining remodeling but little known about its anti-inflammatory potential colitis. We investigated effect systemically administered BMP7 corticosteroids on severity inflammation, macrophage differentiation, regeneration...
Xenogeneic biomaterials Cerbone® and OsteoBiol® are widely used in oral implantology. In dental practice, xenogeneic biomaterial is usually combined with autologous bone to provide volume stability needed for long-term implants. Magnesium alloy implants dissolve form mineral corrosion layer that directly contact tissue, allowing deposition of the newly formed bone. CSBD heals by intramembranous ossification therefore a convenient model analyses ostoconductive osteoinductive properties...
Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)