A5016511178- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Parasites and Host Interactions
- Hepatitis B Virus Studies
- Peptidase Inhibition and Analysis
- Liver Diseases and Immunity
- Protease and Inhibitor Mechanisms
- Hepatitis C virus research
- Drug Transport and Resistance Mechanisms
- Liver Disease and Transplantation
- Endoplasmic Reticulum Stress and Disease
- Pediatric Hepatobiliary Diseases and Treatments
- Cystic Fibrosis Research Advances
- Inflammatory Bowel Disease
- Drug-Induced Hepatotoxicity and Protection
- Helminth infection and control
- Macrophage Migration Inhibitory Factor
- Mycobacterium research and diagnosis
- Pancreatitis Pathology and Treatment
- Parasite Biology and Host Interactions
- Cytokine Signaling Pathways and Interactions
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Helicobacter pylori-related gastroenterology studies
- Cell Adhesion Molecules Research
- Trace Elements in Health
Justus-Liebig-Universität Gießen
2016-2025
Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori
2022
Istituti di Ricovero e Cura a Carattere Scientifico
2022
Universitätsklinikum Gießen und Marburg
2006-2021
Giessen School of Theology
2021
Universitätsklinikum Aachen
2003-2015
Vrije Universiteit Brussel
2008
Morpho (United States)
2008
University of Antwerp
2008
RWTH Aachen University
2004-2006
Tissue inhibitor of metalloproteinases-1 (TIMP-1) plays a crucial role in the pathogenesis hepatic fibrosis and thus may represent an important therapeutic target design anti-fibrotic strategies for chronic liver disease. We present innovative therapy based on assignment inactivated enzymes acting as scavengers TIMP-1. Hepatic was induced BALB/c mice by repetitive intraperitoneal CCl4 injection. The animals were treated with proteolytic inactive matrix metalloproteinase-9 mutants (E402Q,...
Matrix metalloproteinases (MMPs) are responsible for the turnover and degradation of extracellular matrix. They play a crucial role in growth migration colorectal carcinoma cells. Colorectal carcinomas characterized by enhanced expression MMP-2, MMP-9, MMP-7, MMP-13. The aim this study was to determine levels MMP-13, MMP-14 their specific inhibitor TIMP-1 inflammatory bowel diseases precancerous lesions colon, i.e., Crohn's disease ulcerative colitis, adenomatous polyps (APs) comparison....
Bone marrow (BM)-derived stem cells and CD34+ fibrocytes are associated with fibrogenesis in several organs. In an Abcb4 −/− mouse model for sclerosing cholangitis alpha-smooth muscle actin-positive (α-SMA+) myofibroblasts thought to play a pivotal role hepatic fibrogenesis. The aim of this study was 2-fold: (1) demonstrate that the origin important fibrogenetic cell population is BM; (2) investigate whether transplantation BM (BM-Tx) affects liver function, staging, grading. Surrogate...
Clinical data have provided evidence that schistosomiasis can promote hepatocellular carcinogenesis. c‐Jun and STAT3 are critical regulators of liver cancer development progression. The aim the present study was to investigate activation by Schistosoma mansoni infection. Expression function as well proliferation DNA repair were analyzed western blotting, electrophoretic mobility‐shift assay, immunohistochemistry in S. –infected hamsters, Huh7 cells, primary hepatocytes, human biopsies....
Hepatocyte growth factor (HGF) gene transfer inhibits liver fibrosis by regulating aberrant cellular functions, while mutant matrix metalloproteinase-9 (mMMP-9) enhances degradation neutralizing the elevated tissue inhibitor of metalloproteinase-1 (TIMP-1). It was shown that ASH1 and EZH2 methyltransferases are involved in development fibrosis; however, their role resolution phase has not been investigated. This study evaluated two mechanistically different therapeutic modalities, HGF mMMP-9...
Abstract Expression patterns of matrix metalloproteinase-9 (MMP-9) and its specific inhibitor, tissue inhibitor metalloproteinases-1 (TIMP-1), are closely correlated with physiological pathological processes characterized by the degradation accumulation extracellular (ECM). Both, activated MMP-9 pro-MMP-9 can bind to TIMP-1, most cell types secrete in complex TIMP-1. Utilizing immunofluorescence, we observed intracellular co-localization TIMP-1 stimulated human fibrosarcoma cells. In present...
About 30% of patients with Cystic Fibrosis (CF) develop CF-associated liver disease (CFLD). Recent studies have shown that transient elastography (TE), as a method to quantify stiffness, allows non-invasive diagnosis CFLD in adults and children CF. Within this study we aimed prospectively identify at risk for development by longitudinal analysis stiffness fibrosis scores 5-year follow-up. 36 pediatric 16 adult initial below the cut-off value indicative (6.3 kPa) were examined 4-5 years. TE,...
The endocannabinoid (EC) system has been implicated in the pathogenesis of several metabolic diseases, including nonalcoholic fatty liver disease (NAFLD). With current study we aimed to verify modulatory effect receptor 1 (CB1)-signaling on perilipin 2 (PLIN2)-mediated lipophagy. Here, demonstrate that a global knockout cannabinoid gene (CB1−/−) reduced expression lipid droplet binding protein PLIN2 livers CB1−/− and hepatitis B surface (HBs)-transgenic mice, which spontaneously develop...
Mesenchymal stromal cells (MSC) increasingly emerge as an option to ameliorate non-alcoholic steatohepatitis (NASH), a serious disease, which untreated may progress liver cirrhosis and cancer. Before clinical translation, the mode of action MSC needs be established. Here, we established NASH in immune-deficient mouse model by feeding high fat diet. Human bone-marrow-derived were delivered via intrasplenic transplantation. As verified biochemical image analyses, human mesenchymal improved...
Objective. Matrix metalloproteinases (MMPs) are considered the predominant proteases in pathogenesis of mucosal ulcerations associated with inflammatory bowel disease (IBD). Whether malignancy MMP-7 and MMP-13 or recently cloned MMP-28 convey a certain meaning for intestinal homeostasis IBD is currently unknown. We therefore set off to analyze regulation patterns cellular origins these MMPs tissues patients ulcerative colitis (UC). Material methods. Biopsy samples affected healthy were...
Reports on the effects of bone marrow-derived cells hepatic fibrosis are contradictory. Impaired but increased inflammation has recently been demonstrated 10 weeks after marrow transplantation (BM-Tx) in Abcb4-/- mice. It is hypothesised that BM-Tx might have long-term therapeutic potential by altering immunological and matrix remodelling processes leading to regeneration.After lethal irradiation recipient mice, BM from GFP+ donor mice (allogeneic Tx) or (syngeneic were transplanted via tail...
Cathepsin L (CTSL) and B (CTSB) have a crucial role in extracellular matrix (ECM) degradation tissue remodeling, which is prominent feature of fibrogenesis. The aim this study was to determine the clinical significance these cathepsins liver fibrosis.Hepatic histological CTSL CTSB expression were assessed experimental models fibrosis, patients with cirrhosis, chronic viral hepatitis, controls by real-time PCR immunohistochemistry. Plasma levels analyzed 51 cirrhosis (Child-Pugh stages A, C)...
Schistosomiasis is a neglected tropical disease caused by worm parasites of the genus Schistosoma. Upon infection, parasite eggs can lodge inside host organs like liver. This leads to granuloma formation, which main cause pathology schistosomiasis. To better understand different levels host–pathogen interaction and pathology, our study focused on characterization glycosphingolipids (GSLs). For this purpose, GSLs in livers infected noninfected hamsters were studied combining...
Background and Aims Cystic Fibrosis associated liver disease (CFLD) develops in approximately 30% of CF patients. However, routine sensitive diagnostic tools for CFLD are lacking. Within this study, we aimed to identify new experimental biomarkers the detection CFLD. Methods 45 patients were included study received transient elastography. Differential regulation 220 different serum proteins was assessed a subgroup with without Most interesting candidate further quantified validated by ELISA...