A5078543959- Cholangiocarcinoma and Gallbladder Cancer Studies
- Radiomics and Machine Learning in Medical Imaging
- Colorectal Cancer Treatments and Studies
- Genetic factors in colorectal cancer
- Cancer Cells and Metastasis
- AI in cancer detection
- Lymphoma Diagnosis and Treatment
- Occupational and environmental lung diseases
- Cancer Research and Treatments
- Urinary and Genital Oncology Studies
- Advanced X-ray and CT Imaging
- Bladder and Urothelial Cancer Treatments
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Hepatitis C virus research
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Salivary Gland Tumors Diagnosis and Treatment
- Genital Health and Disease
- Gallbladder and Bile Duct Disorders
- Prostate Cancer Diagnosis and Treatment
- Tissue Engineering and Regenerative Medicine
- Pancreatic and Hepatic Oncology Research
- Colorectal and Anal Carcinomas
- Parasites and Host Interactions
Goethe University Frankfurt
2016-2025
University Hospital Frankfurt
2016-2025
Humboldt-Universität zu Berlin
2024-2025
Charité - Universitätsmedizin Berlin
2024-2025
Freie Universität Berlin
2024-2025
Frankfurt Cancer Institute
2023
Robert Koch Institute
2016
In colorectal cancers, the tumor microenvironment plays a key role in prognosis and therapy efficacy. Patient-derived organoids (PDTO) show enormous potential for preclinical testing; however, cultured cells lose important characteristics, including consensus molecular subtypes (CMS). To better reflect cellular heterogeneity, we established cancer organoid-stroma biobank of matched PDTOs cancer-associated fibroblasts (CAF) from 30 patients. Context-specific phenotyping showed that...
Recently, a transcriptome-based consensus molecular subtype (CMS) classification of colorectal cancer (CRC) has been established, which may ultimately help to individualize CRC therapy. However, the lack animal models that faithfully recapitulate different subtypes impedes adequate preclinical testing stratified therapeutic concepts. Here, we demonstrate constitutive AKT activation in intestinal epithelial cells markedly enhances tumor invasion and metastasis Trp53ΔIEC mice...
Muscle-invasive urothelial bladder cancer (MIBC) is associated with limited response rates to systemic therapy, risk of recurrence and death. Tumor infiltrating immune cells have been outcome chemo-and immunotherapy in MIBC. We aimed profile the tumor microenvironment (TME) predict prognosis MIBC responses adjuvant chemotherapy.We performed multiplex immunohistochemistry (IHC) profiling quantification stromal (CD3, CD4, CD8, CD163, FoxP3, PD-1, CD45, Vimentin, αSMA, PD-L1, Pan-Cytokeratin,...
Summary Many epidemiological studies provide solid evidence for an association of chronic hepatitis C virus ( HCV ) infection with B‐cell non‐Hodgkin's lymphoma (B‐ NHL ). However, the most convincing a causal relationship between and development is observation B‐ regression after eradication by antiviral therapy AVT We conducted literature search to identify that included patients ‐associated ‐ who received , intention treat viral disease at same time. The primary end point was correlation...
Prostate cancer is a major health concern in aging men. Paralleling an society, prostate prevalence increases emphasizing the need for efficient diagnostic algorithms.Retrospectively, 106 tissue samples from 48 patients (mean age, [Formula: see text] years) were included study. Patients suffered (n = 38) or benign prostatic hyperplasia 10) and treated with radical prostatectomy Holmium laser enucleation of prostate, respectively. We constructed microarrays (TMAs) comprising representative...
Inflammation promotes solid tumor progression, but how regulatory mechanisms of inflammation may affect leukemia is less well studied. Using annexin A5 (ANXA5), a calcium-binding protein known for apoptosis, which we discovered to be differentially expressed in the bone marrow microenvironment (BMM) mice with acute myeloid (AML) vs chronic leukemia, as model system, unravel here circuit AML-derived necrosis factor α (TNF-α) dose-dependently reduces ANXA5 BMM. This creates an inflammatory BMM...
Abstract Objectives To analyze the performance of radiological assessment categories and quantitative computational analysis apparent diffusion coefficient (ADC) maps using variant machine learning algorithms to differentiate clinically significant versus insignificant prostate cancer (PCa). Methods Retrospectively, 73 patients were included in study. The (mean age, 66.3 ± 7.6 years) examined with multiparametric MRI (mpMRI) prior radical prostatectomy ( n = 33) or targeted biopsy 40). index...
We aimed to identify hepatocellular carcinoma (HCC) patients who will respond repetitive transarterial chemoembolization (TACE) improve the treatment algorithm. Retrospectively, 61 (mean age, 65.3 years ± 10.0 [SD]; 49 men) with 94 HCC mRECIST target-lesions had three consecutive TACE between 01/2012 and 01/2020 were included. Robust non-redundant radiomics features extracted from 24 h post-embolization CT. Five different clinical TACE-scores assessed. Seven feature selection methods machine...
// Dirk Walter 1, 2 , Claudia Döring Magdalena Feldhahn 3 Florian Battke Sylvia Hartmann Ria Winkelmann Markus Schneider Katrin Bankov 1 Andreas Schnitzbauer 4 Stefan Zeuzem Martin Leo Hansmann Jan Peveling-Oberhag 2, 5 Department of Internal Medicine I, Johann Wolfgang Goethe-University Hospital, 60590 Frankfurt, Germany Dr. Senckenberg Institute Pathology, CeGaT GmbH, 72076 Tuebingen, General and Visceral Surgery, for Gastroenterology, Hepatology Endocrinology, Robert-Bosch 70376...
Abstract Purpose: The clinical standard treatment for patients with malignant pleural mesothelioma (MPM) includes a cisplatin-based chemotherapy, leading to reduction of tumor size in only minority patients. Predicting response chemotherapy MPM by using genetic marker would, therefore, enable patient stratification. Experimental Design: In this retrospective biomarker study, eligible had resectable MPM, measurable disease, and available primary tissue. All underwent first-line cisplatin...
In gastric cancer (GC), there are four molecular subclasses that indicate whether patients respond to chemotherapy or immunotherapy, according the TCGA. clinical practice, however, not every patient undergoes testing. Many laboratories have used well-implemented in situ techniques (IHC and EBER-ISH) determine their cohorts. Although multiple stains used, we show a staining approach is unable correctly discriminate all subclasses. As an alternative, trained ensemble convolutional neuronal...
Molecular markers for predicting prognosis of colorectal cancer (CRC) patients are urgently needed effective disease management. We reported previously that the multifunctional enzyme Transglutaminase 2 (TGM2) is essential CRC cell survival by inactivation tumor suppressor p53. Based on these data, we determined clinical relevance TGM2 expression and explored its potential as prognostic marker therapeutic target in CRC. profiled protein samples 279 clinically characterized using...
Different studies have characterized the microenvironment and its prognostic impact in classic Hodgkin lymphoma whereas such analyses are pending for nodular lymphocyte-predominant (NLPHL). We thus investigated characteristics of tumour cells NLPHL evaluated possible correlations with clinical presentation. Lymph node samples from 152 patients who had first-line treatment within randomized German Study Group HD16-HD18 trials were available analysed regard to IgD status nuclear size as well...
Colorectal cancers (CRCs) deficient in the DNA mismatch repair protein MutL homolog 1 (MLH1) display distinct clinicopathological features and require a different therapeutic approach compared to CRCs with MLH1 proficiency. However, molecular basis of this fundamental difference remains elusive. Here, we report that MLH1-deficient exhibit reduced levels cytoskeletal scaffolding non-erythroid spectrin αII (SPTAN1), tumor progression metastasis correlate SPTAN1 levels.To investigate link...
The induction of apoptosis is a direct way to eliminate tumor cells and improve cancer therapy. Apoptosis tightly controlled by the balance pro- antiapoptotic Bcl-2 proteins. BH3 mimetics neutralize function proteins are highly promising compounds inducing in several entities including pediatric malignancies. However, clinical application solid tumors impeded frequent resistance single anticipated toxicity high concentrations or combination treatments. One potential avenue increase potency...
Introduction and Objective Identifying patients that benefit from cisplatin-based adjuvant chemotherapy is a major issue in the management of muscle-invasive bladder cancer (MIBC). The purpose this study to correlate “luminal” “basal” type protein expression with histological subtypes, investigate prognostic impact on survival after define molecular consensus subtypes “double negative” (i.e., without CK5/6 or GATA3). Materials Methods We performed immunohistochemical (IHC) analysis GATA3 for...
Muscle-invasive bladder cancer (MIBC) is associated with limited response rates to systemic therapy leading a significant risk of recurrence and death. A recently discovered histone methyltransferase KMT9, acts as an epigenetic regulator carcinogenesis in different tumor entities. In this study, we investigated the presence association histological molecular subtypes their impact on survival KMT9α MIBC. We performed immunohistochemical (IHC) analysis 135 MIBC patients undergoing radical...
Abstract Purpose Dedicated gene signatures in small (SD-iCCA) and large (LD-iCCA) duct type intrahepatic cholangiocarcinoma remain unknown. We performed immune profiling SD- LD-iCCA to identify novel biomarker candidates for personalized medicine. Methods Retrospectively, 19 iCCA patients with either SD-iCCA (n = 10, median age, 63.1 years (45–86); men, 4) or 9, 69.7 (62–85); 5)) were included. All diagnosed histologically confirmed between 04/2009 01/2021. Tumor tissue samples processed...
Abstract Purpose The newly discovered histone methyltransferase KMT9 serves as an epigenetic regulator of carcinogenesis in various cancer entities. For the first time, we investigated presence KMT9α cholangiocarcinoma, association with histologic subtypes, and its impact on survival. Methods A tissue microarray cohort all CCA patients who underwent surgical resection curative intent between 08/2005 12/2021 at University Hospital Frankfurt was immunohistochemically analyzed antibody. overall...
Distinct immune patterns of hepatocellular carcinoma (HCC) may have prognostic implications in the response to transarterial chemoembolization (TACE). Thus, we aimed exploratively analyze tumor tissue HCC patients who do or not respond TACE, and identify novel biomarkers predictive TACE. We retrospectively included 15 had three consecutive TACE between January 2019 November 2019. Eight a while seven no All measurable disease according mRECIST. Corresponding samples were processed for...
Non-small-cell lung cancer (NSCLC) shows a high incidence of brain metastases (BM). Early detection is crucial to improve clinical prospects. We trained and validated classifier models identify patients with risk developing BM, as they could potentially benefit from surveillance MRI.