A5010107827- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Legume Nitrogen Fixing Symbiosis
- Transgenic Plants and Applications
- Plant Molecular Biology Research
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Epigenetics and DNA Methylation
- Cassava research and cyanide
- Polysaccharides and Plant Cell Walls
- Lichen and fungal ecology
- Bacteriophages and microbial interactions
- Renal and related cancers
- Plant pathogens and resistance mechanisms
- Insect Resistance and Genetics
- Cancer-related gene regulation
- Plant Stress Responses and Tolerance
- Congenital Anomalies and Fetal Surgery
- Alcoholism and Thiamine Deficiency
- Antimicrobial Peptides and Activities
- Knee injuries and reconstruction techniques
- Reproductive Biology and Fertility
- Histone Deacetylase Inhibitors Research
- Metabolism and Genetic Disorders
- Sexual Differentiation and Disorders
University of Kentucky
2024
Innovative Genomics Institute
2019-2020
University of California, Berkeley
2019-2020
Australian National University
2018
University of California, Davis
2013-2018
University of California, Riverside
2017-2018
Joint BioEnergy Institute
2016
University of California System
2015
Boston University
2015
Brigham and Women's Hospital
2011
Surveillance of the extracellular environment by immune receptors is central importance to eukaryotic survival. The rice receptor kinase XA21, which confers robust resistance most strains Gram-negative bacterium Xanthomonas oryzae pv. (Xoo), representative a large class cell surface in plants and animals. We report identification previously undescribed Xoo protein, called RaxX, required for activation XA21-mediated immunity. that lack or carry mutations single RaxX tyrosine residue (Y41),...
During male sexual differentiation, the transforming growth factor-β (TGF-β) signaling molecule anti-Müllerian hormone (AMH; also known as Müllerian inhibiting substance, MIS) is secreted by fetal testes and induces regression of ducts, primordia female reproductive tract organs. Currently, molecular identity downstream events regulated AMH pathway remains unclear. We found that male-specific Wnt4 expression in mouse duct mesenchyme depends upon signaling, implicating WNT a mediator...
Thiamin diphosphate (ThDP), also known as vitamin B1, serves an enzymatic cofactor in glucose metabolism, the Krebs cycle, and branched-chain amino acid biosynthesis all living organisms. Unlike plants microorganisms, humans are not able to synthesize ThDP de novo must obtain it from their diet. Staple crops such rice poor sources of thiamin. Hence, populations that mainly consume commonly suffer thiamin deficiency. In addition thiamin's nutritional function, studies have shown some genes...
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Neural tube defects (NTDs) is a general term for central nervous system malformations secondary to failure of closure or development the neural tube. The resulting pathologies may involve brain, spinal cord and/or vertebral column, in addition associated structures such as soft tissue skin. condition reported among more common birth humans, leading significant infant morbidity and mortality. etiology remains poorly understood but genetic, nutritional, environmental factors, combination...
Posttranslational modification (PTM) of proteins and peptides is important for diverse biological processes in plants animals. The paucity heterologous expression systems PTMs the technical challenges associated with chemical synthesis these modified has limited detailed molecular characterization therapeutic applications. Here we describe an optimized system tyrosine-sulfated Escherichia coli its application a bio-based crop protection strategy rice.
Rice ( Oryza sativa ) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. (Xoo) infection. We previously demonstrated that a chimeric protein containing ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and endodomain (EFR:XA21) in rice does not confer robust resistance Xoo . To test if is required for resistance, we produced transgenic lines consisting of EFR (XA21:EFR) inoculated these with also tested XA21:EFR respond synthetic sulfated 21 amino...
Abstract Indole-3-acetic acid (IAA) plays a critical role in regulating numerous aspects of plant growth and development. While there is much genetic support for tryptophan-dependent (Trp-D) IAA synthesis pathways, little evidence tryptophan-independent (Trp-I) pathways. Using Arabidopsis, we identified two mutant alleles ISS1 (IndoleSevereSensitive) that display indole-dependent overproduction phenotypes including leaf epinasty adventitious rooting. Stable isotope labeling showed iss1, but...
Journal Article Notes on Cephaleuros Get access N. THOMAS ROYAL COLLEGE OF SCIENCELONDON Search for other works by this author on: Oxford Academic PubMed Google Scholar Annals of Botany, Volume os-27, Issue 4, October 1913, Pages 781–792, https://doi.org/10.1093/oxfordjournals.aob.a089487 Published: 01 1913
The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. ( Xoo ). We developed a detached leaf infection assay quickly and reliably measure activation of XA21-mediated immune response using genetic markers. used RNA sequencing elf18 treated EFR:XA21:GFP plants identify candidate genes that could serve as markers for activation. From this analysis, we identified eight are up-regulated in both leaves infected leaves. These results provide rapid...
The rice immune receptor XA21 confers resistance to Xanthomonas oryzae pv. (Xoo), the causal agent of bacterial leaf blight. We previously demonstrated that an auxilin-like protein, BINDING PROTEIN 21 (XB21), positively regulates Xoo. To further investigate function XB21, we performed a yeast two-hybrid screen. identified 22 unique XB21 interacting proteins, including LEUCINE-RICH REPEAT 1 (LRR1), which selected for analysis. Silencing LRR1 in genetic background (XA21-LRR1Ri) compromises Xoo...
The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. ( Xoo ). We developed a detached leaf infection assay quickly and reliably measure activation of XA21-mediated immune response using genetic markers. used RNA sequencing elf18 treated EFR:XA21:GFP plants identify candidate genes that could serve as markers for activation. From this analysis we identified 8 are up-regulated in both leaves infected leaves. These results provide rapid...
Joint injury is associated with protracted atrophy and weakness that poorly recovers; our objective was to define molecular effectors of lost muscle size, strength, plasticity following joint injury. We hypothesized epigenetic imprinting in impairs contributes poor functional recovery surgical repair. recruited a total sixteen ACL-injured participants from whom we obtained vastus lateralis biopsies Pre-, 7-days post-, 4-months post- reconstruction surgery (Sx) strength measures up 6-months...
Abstract Xanthomonas species, Pseudomonas syringae and Ralstonia solanacearum are bacterial plant pathogens that cause significant yield loss in many crop species. Current control methods for these insufficient but there is potential generating new disease-resistant varieties. Plant immune receptors encoded by nucleotide-binding, leucine-rich repeat (NLR) genes typically confer resistance to produce a cognate elicitor, often an effector protein secreted the pathogen promote virulence. The...
Abstract IAA plays a critical role in regulating numerous aspects of plant growth and development. While there is much genetic support for tryptophan-dependent (Trp-D) synthesis pathways, little evidence tryptophan-independent (Trp-I) pathways. Using Arabidopsis, we identified two mutant alleles ISS1 ( I ndole S evere ensitive ) that display indole-dependent overproduction phenotypes including leaf epinasty adventitious rooting. Stable isotope labeling showed iss1 , but not WT, uses...
The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. ( Xoo ). We developed a detached leaf infection assay quickly and reliably measure activation of XA21-mediated immune response using genetic markers. used RNA sequencing elf18 treated EFR:XA21:GFP plants identify candidate genes that could serve as markers for activation. From this analysis we identified 8 are up-regulated in both leaves infected leaves. These results provide rapid...
Summary Rice ( Oryza sativa ) plants expressing the XA21 cell surface receptor kinase are resistant to Xanthomonas oryzae pv. Xoo infection. We previously demonstrated that a chimeric protein containing EFR E LONGATION F ACTOR Tu R ECEPTOR) ectodomain and endodomain (EFR:XA21) in rice does not confer robust resistance . To test if is required for resistance, we produced transgenic lines consisting of (XA21:EFR) inoculated these with also tested XA21:EFR respond synthetic sulfated 21 amino...