A5022666421- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogenic Bacteria Studies
- Gut microbiota and health
- Agricultural Development and Management
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant Virus Research Studies
- Plant responses to water stress
- Plant pathogens and resistance mechanisms
- Modular Robots and Swarm Intelligence
- Polysaccharides and Plant Cell Walls
- Microbial Community Ecology and Physiology
- Photosynthetic Processes and Mechanisms
- Transgenic Plants and Applications
- 3D Printing in Biomedical Research
- Agricultural Systems and Practices
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Nematode management and characterization studies
- Plant Micronutrient Interactions and Effects
- Augmented Reality Applications
- Spaceflight effects on biology
- Genetic Mapping and Diversity in Plants and Animals
- CRISPR and Genetic Engineering
University of California, Berkeley
2018-2024
Plant Gene Expression Center
2020-2024
Agricultural Research Service
2020-2023
United States Department of Agriculture
2023
University of California, Davis
2012-2018
University of Illinois Urbana-Champaign
2018
University of California, San Diego
2009-2012
Abscisic acid (ABA) mediates resistance to abiotic stress and controls developmental processes in plants. The group-A PP2Cs, of which ABI1 is the prototypical member, are protein phosphatases that play critical roles as negative regulators very early ABA signal transduction. Because redundancy thought limit genetic dissection signalling, identify redundant signalling proteins, we pursued a proteomics approach. We generated YFP-tagged Arabidopsis expression lines identified vivo...
Surveillance of the extracellular environment by immune receptors is central importance to eukaryotic survival. The rice receptor kinase XA21, which confers robust resistance most strains Gram-negative bacterium Xanthomonas oryzae pv. (Xoo), representative a large class cell surface in plants and animals. We report identification previously undescribed Xoo protein, called RaxX, required for activation XA21-mediated immunity. that lack or carry mutations single RaxX tyrosine residue (Y41),...
Abstract Recent studies have demonstrated that drought leads to dramatic, highly conserved shifts in the root microbiome. At present, molecular mechanisms underlying these responses remain largely uncharacterized. Here we employ genome-resolved metagenomics and comparative genomics demonstrate carbohydrate secondary metabolite transport functionalities are overrepresented within drought-enriched taxa. These data also reveal bacterial iron metabolism functionality is correlated with...
Abstract Host genetics has recently been shown to be a driver of plant microbiome composition. However, identifying the underlying genetic loci controlling microbial selection remains challenging. Genome-wide association studies (GWAS) represent potentially powerful, unbiased method identify microbes sensitive host genotype and connect them with that influence their colonization. Here, we conducted population-level analysis rhizospheres 200 sorghum genotypes. Using 16S rRNA amplicon...
Plants are continuously challenged by pathogens including viruses, bacteria, and fungi. The plant immune system recognizes invading responds activating an response. These responses occur rapidly often involve post-translational modifications (PTMs) within the proteome. Protein phosphorylation is a common intensively studied form of these PTMs regulates many processes growth, development, immunity. Most well-characterized pattern recognition receptors (PRRs), Xanthomonas resistance 21,...
A crewed mission to and from Mars may include an exciting array of enabling biotechnologies that leverage inherent mass, power, volume advantages over traditional abiotic approaches. In this perspective, we articulate the scientific engineering goals constraints, along with example systems, guide design a surface biomanufactory. Extending past arguments for exploiting stand-alone elements biology, argue integrated biomanufacturing plant replete modules microbial in situ resource utilization,...
Plants compete for light partly by over-producing chlorophyll in leaves. The resulting high absorption is an effective strategy out competing neighbors mixed communities, but it prevents transmission to lower leaves and limits photosynthesis dense agricultural canopies. We used a CRISPR/Cas9-mediated approach engineer rice plants with truncated light-harvesting antenna (TLA) via knockout mutations individual assembly component genes CpSRP43, CpSRP54a, its paralog, CpSRP54b. compared the...
Summary The Arabidopsis guard cell anion channel SLAC 1 is essential for stomatal closure in response to various endogenous and environmental stimuli. Interestingly, here we reveal an unexpected impairment of slac1 alleles on opening. We report that mutations unexpectedly slow opening induced by light, low CO 2 elevated air humidity intact plants this caused the severely reduced activity inward K + (K ) channels cells. Expression transporters involved showed small but significant reductions...
The intimate interaction between plant host and associated microorganisms is crucial in determining fitness, can foster improved tolerance to abiotic stresses diseases. As the microbiome be highly complex, low-cost, high-throughput methods such as amplicon-based sequencing of 16S rRNA gene are often preferred for characterizing its microbial composition diversity. However, selection appropriate methodology when conducting experiments critical reducing biases that make analysis comparisons...
Plant-associated microbial communities shift in composition as a result of environmental perturbations, such drought. It has been shown that Actinobacteria are enriched plant roots and rhizospheres during drought stress. However, the correlations between microbiome dynamics response to poorly understood. Here we apply combination bacterial community analysis metabolite profiling Sorghum bicolor roots, rhizospheres, soil recovery investigate potential contributions host metabolism shifts...
Abstract Plant microbiome engineering remains a significant challenge due to challenges associated with accurately predicting assembly and function in complex, heterogeneous soil environments. However, host-mediated selection can simplify the process by using plant host phenotype as reporter of function; iteratively selecting microbiomes from hosts desired phenotypes them inoculate subsequent cohorts hosts, artificial steer towards composition producing optimized phenotypes. In this study,...
Rice ( Oryza sativa ) plants expressing the XA21 cell-surface receptor kinase are resistant to Xanthomonas oryzae pv. (Xoo) infection. We previously demonstrated that a chimeric protein containing ELONGATION FACTOR Tu RECEPTOR (EFR) ectodomain and endodomain (EFR:XA21) in rice does not confer robust resistance Xoo . To test if is required for resistance, we produced transgenic lines consisting of EFR (XA21:EFR) inoculated these with also tested XA21:EFR respond synthetic sulfated 21 amino...
The rice immune receptor XA21 confers resistance to Xanthomonas oryzae pv. (Xoo), the causal agent of bacterial leaf blight. We previously demonstrated that an auxilin-like protein, BINDING PROTEIN 21 (XB21), positively regulates Xoo. To further investigate function XB21, we performed a yeast two-hybrid screen. identified 22 unique XB21 interacting proteins, including LEUCINE-RICH REPEAT 1 (LRR1), which selected for analysis. Silencing LRR1 in genetic background (XA21-LRR1Ri) compromises Xoo...
Abstract Host genetics has recently been shown to be a driver of plant microbiome composition. However, identifying the underlying genetic loci controlling microbial selection remains challenging. Genome wide association studies (GWAS) represent potentially powerful, unbiased method identify microbes sensitive host genotype, and connect them with that influence their colonization. Here, we conducted population-level analysis rhizospheres 200 sorghum genotypes. Using 16S rRNA amplicon...
A crewed mission to and from Mars may include an exciting array of enabling biotechnologies that leverage inherent mass, power, volume advantages over traditional abiotic approaches. In this perspective, we articulate the scientific engineering goals constraints, along with example systems, guide design a surface biomanufactory. Extending past arguments for exploiting stand-alone elements biology, argue integrated biomanufacturing plant replete modules microbial \textit{in situ} resource...
Abstract Matrix metalloproteinases (MMPs) are zinc‐dependent endo‐peptidases that in mammals known to be involved remodeling the extracellular matrix (ECM) developmental and pathological processes. In this study, we report At5‐MMP of Arabidopsis thaliana important for root development bacterial communities. is mainly localized vasculature lateral root, an T‐DNA insertion mutant ( mmp5 KO ) showed reduced growth a lower number apexes, causing water uptake from soil. Subsequently, sensitive...
Plant microbiome engineering remains a significant challenge due to challenges associated with accurately predicting assembly and function in complex, heterogeneous soil environments. However, host-mediated selection can simplify the process by using plant host phenotype as reporter of function; iteratively selecting microbiomes from hosts desired phenotypes them inoculate subsequent cohorts hosts, artificial steer towards composition producing optimized phenotypes. In this study, we...
Plant microbiome engineering remains a significant challenge due to challenges associated with accurately predicting assembly and function in complex, heterogeneous soil environments. However, host-mediated selection can simplify the process by using plant host phenotype as reporter of function; iteratively selecting microbiomes from hosts desired phenotypes them inoculate subsequent cohorts hosts, artificial steer towards composition producing optimized phenotypes. In this study, we...
While drought severely affects plant growth and crop production, the molecular mechanisms of response plants remain unclear. In this study, we demonstrated for first time effect pseudo-protease AtFtsHi3 Arabidopsis thaliana on overall in tolerance. An AtFTSHi3 knock-down mutant [ftshi3-1(kd)] displayed a pale-green phenotype with lower photosynthetic efficiency Darwinian fitness compared to wild type (Wt). observed delay seed germination ftshi3-1(kd) was attributed overaccumulation abscisic...
ABSTRACT Interactions between plants and their root-associated microbiome are important for determining host fitness during periods of stress. During drought, monoderm bacteria more abundant in sorghum roots than those watered controls. Additionally, a reversion from to diderm dominance occurs drought-stressed one week after rewatering. However, the mechanisms driving this rapid composition shift is currently unknown. To understand if changes metabolism correlated with shift, we employed 16S...
Tyrosine phosphorylation has emerged as an important regulator of plasma membrane-localized immune receptors activity. Here, we investigate the role tyrosine in regulation rice XANTHOMONAS RESISTANCE 21 (XA21)-mediated immunity. We demonstrate that juxtamembrane and kinase domain Escherichia coli –expressed XA21 (XA21JK) autophosphorylates on residues. Directed mutagenesis four out nine residues XA21JK reduced autophosphorylation. These sites include Tyr 698 domain, 786 , 907 909 domain....