A5031145843- Sulfur Compounds in Biology
- Neuropeptides and Animal Physiology
- Chronic Obstructive Pulmonary Disease (COPD) Research
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Obstructive Sleep Apnea Research
- Receptor Mechanisms and Signaling
- Redox biology and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- DNA and Nucleic Acid Chemistry
- Chemical Synthesis and Analysis
- Glutathione Transferases and Polymorphisms
- Reproductive Biology and Fertility
- Amino Acid Enzymes and Metabolism
- Dermatology and Skin Diseases
- Neuroscience and Neuropharmacology Research
- Genetics and Neurodevelopmental Disorders
- Biochemical effects in animals
- Reproductive Physiology in Livestock
- Mitochondrial Function and Pathology
- Alzheimer's disease research and treatments
- Signaling Pathways in Disease
- High Altitude and Hypoxia
- Genomics, phytochemicals, and oxidative stress
University of Tartu
2016-2025
Czech Academy of Sciences, Institute of Physiology
2011
Stockholm University
1998-2007
Estonian Biocentre
2000-2003
Karolinska Institutet
1999-2002
AstraZeneca (Sweden)
2002
We examined the role of hippocampal galanin in an animal model status epilepticus (SE). Control rats showed abundant galanin-immunoreactive (Gal-IR) fibers dentate hilus, whereas no Gal-IR neurons were observed. Three hours after onset self-sustaining SE (SSSE), induced either by intermittent stimulation perforant path for 30 min (PPS) or injection lithium and pilocarpine, disappeared hilus remained absent up to 1 week afterward. Twelve induction PPS 3 hr pilocarpine administration, appeared...
Galanin is a neuropeptide with wide variety of biological functions, including that strong endogenous anticonvulsant. No nonpeptide ligands, capable activating galanin receptors, are available today. Based on known pharmacophores galanin, combinatorial library was designed, synthesized, and screened at the rat hippocampal receptor. A low molecular weight receptor agonist, 7-((9-fluorenylmethoxycarbonyl)cyclohexylalanyllysyl)amino-4-methylcoumarin (galnon) found to displace 125 I-galanin...
Cell-penetrating peptides, CPPs, are used as delivery vectors for pharmacologically interesting substances, such antisense oligonucleotides, proteins and peptides. We present a general principle designing cell-penetrating peptides derived from naturally occurring well randomly generated polyamino acid sequences. Thereby, we introduce novel pharmacological identification of which the applications can be numerous, including cellular transduction mimics intracellular protein–protein...
Carotenoid and melanin pigments in the plumage of birds are hypothesized to be sensitive oxidative stress. We manipulated status captive greenfinches (Carduelis chloris L.) by administration buthionine sulfoximine (BSO), a selective inhibitor synthesis glutathione (GSH), an intracellular antioxidant. Half treated group, as well control also received dietary carotenoid (lutein) supplementation. BSO treatment reduced erythrocyte GSH levels caused damage indicated increased concentration plasma...
Assessment of oxidative stress is an important but technically challenging procedure in medical and biological research. The reactive oxygen metabolites (d-ROMs) test a simple assay marketed for analyzing the total amount hydroperoxides serum via Fenton's reaction. Earlier reports have raised suspicion that part signal detected comes from sources other than generated by stress. aim this study was to identify which components interfere with d-ROMs signal. By application sodium azide,...
Apart from the refined management-oriented clinical stratification of chronic obstructive pulmonary disease (COPD), molecular pathologies behind this highly prevalent have remained obscure. The aim study was characterization patients with COPD, based on metabolomic profiling peripheral blood and exhaled breath condensate (EBC) within context defined demographic variables. Mass-spectrometry-based targeted analysis serum metabolites (mainly amino acids lipid species), untargeted profiles EBC...
Abstract Systematic understanding of the metabolite signature diseases may lead to a closer disease pathogenesis and ultimately development novel therapies diagnostic tools. Here we compared for first time full metabolomic profiles lesional non-lesional skin biopsies obtained from plaque psoriasis patients samples healthy controls. Significant differences in concentration levels 29 metabolites were identified that provide several insights into metabolic pathways psoriatic lesions. The...
Novel analogues of the cell-penetrating peptides penetratin and transportan were synthesized. The distribution biotin-labeled in Bowes melanoma cell line has been investigated by indirect fluorescence with fluorescein-streptavidin detection. time course uptake 125I-labeled characterized same line. Molecular modeling was used to analyze penetration orientation molecules a simulated biological membrane. results, both from molecular studies, imply that do not enter cells related mechanisms they...
Communication between various ovarian cell types is a prerequisite for folliculogenesis and ovulation. In antral follicles granulosa cells divide into two distinct populations of mural cumulus (CGC), enveloping the antrum surrounding oocyte, respectively. Both types, with compartment in excess, contribute to floating (FGC) population follicular fluid. The aim this study was compare transcriptomes FGC CGC stimulated obtained from 19 women undergoing IVF–ICSI procedure. were fluid during...
Summary The Arabidopsis guard cell anion channel SLAC 1 is essential for stomatal closure in response to various endogenous and environmental stimuli. Interestingly, here we reveal an unexpected impairment of slac1 alleles on opening. We report that mutations unexpectedly slow opening induced by light, low CO 2 elevated air humidity intact plants this caused the severely reduced activity inward K + (K ) channels cells. Expression transporters involved showed small but significant reductions...
The aim of present study was to find genetic pathways activated during infection with bacterial meningitis (BM) and potentially influencing the course using genome-wide RNA expression profiling combined pathway analysis functional annotation differential transcription.We analyzed 21 patients BM hospitalized in 2008. control group consisted 18 healthy subjects. extracted from whole blood, globin mRNA depleted gene performed GeneChip Human Gene 1.0 ST Arrays which can assess transcription...
Acylcarnitines (ACs) have been shown to a potential activate pro-inflammatory signaling pathways and foster the development of insulin resistance. The first task current study was full list ACs (from C2 C18) in episode psychosis (FEP) patients before after antipsychotic treatment. second relate inflammatory metabolic biomarkers established same patient cohort as our previous studies. Serum levels were determined with AbsoluteIDQ p180 kit (BIOCRATES Life Sciences AG, Innsbruck, Austria) using...
Atopic dermatitis is a chronic inflammatory disease which usually starts in the early childhood and ends before adulthood. However up to 3% of adults remain affected by disease. The onset course influenced various genetic environmental factors. Although immune system has great effect on outcome disease, metabolic markers can also try explain background atopic dermatitis. In this study we analyzed serum patients with using both targeted untargeted metabolomics approaches. We found most...
The main objectives of this study were to characterize the metabolomic profile lesional skin patients with atopic dermatitis, and compare it non- dermatitis controls no dermatological disease. Skin-punch biopsies collected from 15 17 controls. Targeted analysis 188 metabolites was conducted. A total 77 their ratios found, which differed significantly between non-lesional members following classes: amino acids, biogenic amines, acylcarnitines, sphingomyelins or phosphatidylcholines, most...
The aim of present study was to describe changes in gene expression the temporal lobe mice induced by deletion Wfs1 gene. Temporal lobes samples were analyzed using Affymetrix Mouse Genome 420 2 GeneChips and profiles functionally annotated with GSEA Ingenuity Pathway Analysis. We found that mutant are significantly smaller (20.9 +/- 1.6 g) than their wild-type counterparts (31.0 0.6 g, P < 0.0001). This difference existed 129S6 C57B6 backgrounds. Interestingly, microarray analysis...
E-cigarettes are widely believed to be safer than conventional cigarettes and have been even suggested as aids for smoking cessation. However, while reasonable with some regards, this judgment is not yet supported by adequate biomedical research data. Since bronchial epithelial cells the immediate target of inhaled toxicants, we hypothesized that exposure e-cigarettes may affect metabolome human (HBEC) changes are, at least in part, induced oxidant-driven mechanisms. Therefore, evaluated...
Abstract Hypoxia is a contributing factor for the morbidity and mortality in patients with obstructive sleep apnea (OSA). We aimed at identifying percentage of time oxyhemoglobin desaturation below 90% (Tc90%) breakpoint from which most significant changes occur systemic metabolome OSA. In prospective observational study on polysomnography–confirmed symptomatic OSA, profiles 186 metabolites including amino acids, biogenic amines, acylcarnitines (AC), lysophosphatidylcholines,...
Glutathione (GSH) is the major low-molecular weight antioxidant in mammalian cells. Thus, its analogues carrying similar and/or additional positive properties might have clinical perspectives. Here, we report design and synthesis of a library tetrapeptidic GSH called UPF peptides. Compared to cellular our designed peptidic showed remarkably higher hydroxyl radical scavenging ability (EC50 GSH: 1231.0 ± 311.8 μM; EC50 peptides: from 0.03 35 μM) improved antiradical efficiency towards stable...