A5039631092- Advanced Fluorescence Microscopy Techniques
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Insect Resistance and Genetics
- Additive Manufacturing and 3D Printing Technologies
- Forensic Entomology and Diptera Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Neurobiology and Insect Physiology Research
- Laser-Matter Interactions and Applications
- Optical Coherence Tomography Applications
- Insect behavior and control techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Laser Material Processing Techniques
- Congenital Heart Disease Studies
- Advanced Photonic Communication Systems
- Developmental Biology and Gene Regulation
- Adolescent and Pediatric Healthcare
- Near-Field Optical Microscopy
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Advanced Biosensing Techniques and Applications
- Reproductive System and Pregnancy
- Marine Biology and Environmental Chemistry
Delft University of Technology
2022-2025
European Molecular Biology Laboratory
2018-2023
European Molecular Biology Laboratory
2017-2022
Heidelberg University
2016-2018
Heidelberg University
2018
University of Konstanz
2012-2013
Abstract Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation biological entities is often required for a deeper functional understanding underlying process. Here we present home-built integrated framework optical design that combines three-dimensional light-sheet over time with precise manipulations induced by short infrared laser pulses. We demonstrate their potential sub-cellular...
Abstract For organs to be functional, the cells and tissues that constitute them must effectively interact with each other coordinate their behaviours. Halfway during Drosophila embryogenesis, two lateral epidermal sheets stretch fuse at dorsal midline; concomitant this, main tubes of respiratory system also shift towards side embryo. Here, we show these processes occur simultaneously are coordinated by adhesion a subset tracheal trunks common extracellular matrix (ECM) separates them. We...
Extraembryonic tissues contribute to animal development, which often entails spreading over embryo or yolk. Apart from changes in cell shape, the requirements for this tissue are not well understood. Here, we analyze of extraembryonic serosa scuttle fly Megaselia abdita. The forms a columnar blastoderm anlage, becomes squamous epithelium, and eventually spreads proper. We describe dynamics process long-term, whole-embryo time-lapse recordings, demonstrating that free is preceded by prolonged...
Abstract In the present study, influence of topographic and mechanical cues on neuronal growth cones (NGCs) network directionality in 3D‐engineered cell culture models is explored. Two‐photon polymerization (2PP) employed to fabricate nanopillar arrays featuring tunable effective shear modulus. Large variations properties are obtained by altering aspect ratio nanostructures. The seeded with different lines, including neural progenitor cells (NPCs) derived from human induced pluripotent stem...
To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial of microscopes however, limiting factor fluorescence imaging, correspondingly based single molecule localization microscopy (SMLM). Here, we present method to improve precision SMLM by combining point-spread-function engineering with total internal reflection (TIRF) fields decay lengths that vary within on-time fluorophore. Such...
Abstract Understanding the cellular response to DNA strand breaks is crucial decipher mechanisms maintaining integrity of our genome. We present a novel method visualize how mobility nuclear proteins changes in localized damage. are induced via nonlinear excitation with femtosecond laser pulses at λ = 1050 nm 3D‐confined subnuclear volume. After time delay choice, protein within this volume analysed by two‐photon photoactivation PA‐GFP fusion 775 nm. By changing position spot respect zone...
SUMMARY Implantation marks a key transition in mammalian development. The role of embryo-uterus interaction periimplantation development is however poorly understood due to inaccessibility utero. Here, we develop an engineered uterus-like microenvironment recapitulate mouse ex vivo up E5.25 and discover essential integrin-mediated trophoblast adhesion the uterine matrix. Light-sheet microscopy shows that cells undergo Rac1-dependent collective migration upon implantation, displacing...
ABSTRACT Extraembryonic tissues contribute to animal development, which often entails spreading over embryo or yolk. Apart from changes in cell shape, the requirements for this tissue are not well understood. Here we analyze of extraembryonic serosa scuttle fly Megaselia abdita . The forms a columnar blastoderm anlage, becomes squamous epithelium, and eventually spreads proper. We describe dynamics process long-term, whole-embryo time-lapse recordings, demonstrating that free is preceded by...
We employ near-infrared femtosecond pulses to probe local alterations of chromatin dynamics caused by DNA strand breaks. Nonlinear confocal nanomanipulation enables us visualize how damage signaling emanates spatio-temporally in a live cell.
To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial of microscopes however, limiting factor fluorescence imaging, correspondingly based single-molecule localization microscopy. Here, we present method to improve super-resolution SMLM by combining point-spread-function engineering with TIRF fields decay lengths that vary within on-time fluorophore. Such time-varying illumination allows...