A5058619731- 3D Printing in Biomedical Research
- Nanofabrication and Lithography Techniques
- Nanotechnology research and applications
- Additive Manufacturing and 3D Printing Technologies
- Force Microscopy Techniques and Applications
- Supramolecular Self-Assembly in Materials
- Gold and Silver Nanoparticles Synthesis and Applications
- Surface Modification and Superhydrophobicity
- Nanopore and Nanochannel Transport Studies
- Nanomaterials and Printing Technologies
- Graphene and Nanomaterials Applications
- Mechanical and Optical Resonators
- Advanced biosensing and bioanalysis techniques
- Advanced MEMS and NEMS Technologies
- Analytical Chemistry and Sensors
- Bone Tissue Engineering Materials
- Cellular Mechanics and Interactions
- Electrospun Nanofibers in Biomedical Applications
- Quantum Dots Synthesis And Properties
- Laser-Ablation Synthesis of Nanoparticles
- Gas Sensing Nanomaterials and Sensors
- Nonlinear Optical Materials Studies
- Molecular Junctions and Nanostructures
- Nanowire Synthesis and Applications
- Integrated Circuits and Semiconductor Failure Analysis
Delft University of Technology
2019-2025
Utrecht University
2022
Biomedical Primate Research Centre
2022
California Maritime Academy
2021
Centre National de la Recherche Scientifique
2016-2019
Laboratoire d'Analyse et d'Architecture des Systèmes
2016-2019
Université de Toulouse
2016-2019
Institut de Recherches en Technologies et Sciences pour le Vivant
2018
Italian Institute of Technology
2010-2016
Magna Graecia University
2009-2016
Many fatal neurodegenerative diseases such as Alzheimer’s, Parkinson, the prion-related diseases, and non-neurodegenerative disorders type II diabetes are characterized by abnormal amyloid fiber aggregates, suggesting a common mechanism of pathogenesis. We have discovered that class systematically designed natural tri- to hexapeptides with characteristic sequential motif can simulate process assembly further condensation fibrils, probably via unexpected dimeric α-helical intermediate...
The self-assembly of abnormally folded proteins into amyloid fibrils is a hallmark many debilitating diseases, from Alzheimer’s and Parkinson diseases to prion-related disorders diabetes type II. However, the fundamental mechanism aggregation remains poorly understood. Core sequences four seven amino acids within natural that form toxic have been used study amyloidogenesis. We recently reported class systematically designed ultrasmall peptides self-assemble in water cross-β–type fibers. Here...
3D fabrication techniques are rapidly expanding in the field of scaffold development for cell culture and tissue engineering. Herein we report realization free-standing PEGDA hydrogel architectures by using two-photon lithography. The morphological immunofluorescence characterization neuro2A cells revealed a tridimensional colonization featuring multiple neuritic extensions per as well expression β-tubulin neuronal marker actin microfilaments. results open new perspectives continuous quest...
The realization of 3D architectures for the study cell growth, proliferation, and differentiation is a task fundamental importance both technological biological communities involved in development biomimetic culture environments. Here we report fabrication freestanding scaffolds, realized by multiphoton direct laser writing seeded with neuroblastoma cells, their multitechnique characterization using advanced fluorescence imaging approaches. high accuracy process (≈200 nm) allows much finer...
The Poisson's ratio and elastic modulus are two parameters determining the behavior of biomaterials. While effects on cell response is widely studied, very little known regarding ratio. micro-architecture meta-biomaterials determines not only but also several other that influence response, such as porosity, pore size, effective modulus. It is, therefore, challenging to isolate from those micro-architectural parameters. Here, we computationally design with controlled ratios, ranging between...
Abstract The design of advanced functional devices often requires the use intrinsically curved geometries that belong to realm non‐Euclidean geometry and remain a challenge for traditional engineering approaches. Here, it is shown how simple deflection thick meta‐plates based on hexagonal cellular mesostructures can be used achieve wide range intrinsic (i.e., Gaussian) curvatures, including dome‐like saddle‐like shapes. Depending unit cell structure, non‐auxetic positive Poisson ratio) or...
Biomimicking biological niches of healthy tissues or tumors can be achieved by means artificial microenvironments, where structural and mechanical properties are crucial parameters to promote tissue formation recreate natural conditions. In this work, three-dimensional (3D) scaffolds based on woodpile structures were fabricated two-photon polymerization (2PP) different photosensitive polymers (IP-S SZ2080) hydrogels (PEGDA 700) using two 2PP setups, a commercial one customized one. The...
Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. For this reason, besides clinical and preclinical studies, novel in vitro models for assessment response to drugs radiation are being developed. In such context, three-dimensional (3D)-engineered cellular microenvironments, compared unrealistic two-dimensional (2D) monolayer cell culture, provide model closer vivo configuration. Concerning treatment, while X-ray radiotherapy chemotherapy remain current...
Superhydrophobic poly(methyl methacrylate) surfaces with contact angles of ∼170° and high optical X-ray transparencies have been fabricated through the use lithography plasma etching. The contain either a microscale pattern micropillars or random nanofibrillar pattern. Nanoscale asperities on top closely resemble Nelumbo nucifera lotus leafs. evolution angle water lysozyme solution droplets during evaporation was studied micro- nanopatterned surfaces, showing in particular contact-line...
The development of advanced techniques fabrication three-dimensional (3D) microenvironments for the study cell growth and proliferation has become one major motivations material scientists bioengineers in past decade. Here, we present a novel residueless 3D structuration technique poly(dimethylsiloxane) (PDMS) by water-in-PDMS emulsion casting subsequent curing process temperature-/pressure-controlled environment. Scanning electron microscopy X-ray microcomputed tomography allowed us to...
Two-photon polymerization (2PP) has provided the field of cell biology with opportunity to fabricate precisely designed microscaffolds for a wide range studies, from mechanobiology in vitro disease modelling. However, multitude commercial and in-house developed photosensitive materials employed 2PP suffers high auto-fluorescence multiple regions spectrum. In context biological this is major problem since one main methods characterization fluorescence microscopy immuno-stained cells. This...
Here we are presenting a comparative analysis of conformational changes two amyloid β peptides, Aβ(25-35) and Aβ(1-42), in the presence absence phospholipid system, namely, POPC/POPS (1-palmitoyl-2-oleoylphospatidylcholine/palmitoyl-2-oleoylphospatidylserine), through Raman spectroscopy, synchrotron radiation micro Fourier-transform infrared X-ray diffraction. Ringlike samples were obtained from evaporation pure mixed solutions proteins together with system on highly hydrophilic substrates....
A novel electrowetting-on-dielectrics (EWOD) device in open planar geometry allows probing of droplet mixing on a superhydrophobic substrate under quasi contact-free conditions. We demonstrate droplet-based microreactor with integrated convective-flow for the reactive-mixing CaCl2/Na2CO3 solutions. The provides unique conditions scattering, spectroscopy and imaging probes requiring an unobstructed droplet-access.
Abstract A major obstacle in glioma research is the lack of vitro models that can retain cellular features cells vivo. To overcome this limitation, a 3D‐engineered scaffold, fabricated by two‐photon polymerization, developed as cell culture model system to study patient‐derived cells. Scanning electron microscopy, (live cell) confocal and immunohistochemistry are employed assess 3D with respect scaffold colonization, morphology, epidermal growth factor receptor localization. Both established...
Microglia are the resident macrophages of central nervous system and contribute to maintaining brain's homeostasis. Current 2D "petri-dish" in vitro cell culturing platforms employed for microglia, unrepresentative softness or topography native brain tissue. This often contributes changes microglial morphology, exhibiting an amoeboid phenotype that considerably differs from homeostatic ramified healthy To overcome this problem, multi-scale engineered polymeric microenvironments developed...
Glioblastoma (GBM) is a devastating cancer of the brain with an extremely poor prognosis. While X-ray radiotherapy and chemotherapy remain current standard, proton beam therapy appealing alternative as protons can damage cells while sparing surrounding healthy tissue. However, effects on in vitro GBM models at cellular level, especially when co-cultured endothelial cells, building blocks micro-vessels, are still unexplored. In this work, novel 3D-engineered scaffolds inspired by geometry...
The mechanical properties of two-photon-polymerised (2PP) polymers are highly dependent on the employed printing parameters. In particular, features elastomeric polymers, such as IP-PDMS, important for cell culture studies they can influence mechanobiological responses. Herein, we optical-interferometer-based nanoindentation to characterise structures manufactured with varying laser powers, scan speeds, slicing distances, and hatching distances. minimum reported effective Young's modulus...
A novel nanopatterned biocompatible PCL film promotes the growth of primary hippocampal neurons (rendered in blue).