Transforming flat sheets into three-dimensional structures has emerged as an exciting manufacturing paradigm on a broad range of length scales. Among other advantages, this technique permits the use functionality-inducing planar processes starting materials, which after shape-shifting, result in unique combination macro-scale geometry and surface topography. Fabricating arbitrarily complex geometries requires ability to change intrinsic curvature initially structures, while simultaneously...

Individual cells and multicellular systems respond to cell-scale curvatures in their environments, guiding migration, orientation, tissue formation. However, it remains largely unclear how collectively explore pattern complex landscapes with curvature gradients across the Euclidean non-Euclidean spectra. Here, we show that mathematically designed substrates controlled variations induce spatiotemporal organization of preosteoblasts. We quantify curvature-induced patterning find generally...

3D soft bioscaffolds have great promise in tissue engineering, biohybrid robotics, and organ-on-a-chip engineering applications. Though emerging three-dimensional (3D) printing techniques offer versatility for assembling biomaterials, challenges persist overcoming the deformation or collapse of delicate structures during fabrication, especially overhanging thin features. This study introduces a magnet-assisted fabrication strategy that uses magnetic field to trigger shape morphing provide...

Abstract Rapid advances in additive manufacturing have kindled widespread interest the rational design of metamaterials with unique properties over past decade. However, many applications require multi‐physics metamaterials, where multiple are simultaneously optimized. This is challenging since different properties, such as mechanical and mass transport typically impose competing requirements on nano‐/micro‐/meso‐architecture metamaterials. Here, a parametric metamaterial strategy that...

The organization and shape of the microstructural elements trabecular bone govern its physical properties, are implicated in disease, serve as blueprints for biomaterial design. To devise fundamental structure-property relationships design truly bone-mimicking biomaterials, it is essential to characterize structure from perspective geometry, mathematical study shape. Using micro-CT images 70 donors at five different sites, we analyze local global geometry human detail, respectively by...

Abstract The design of advanced functional devices often requires the use intrinsically curved geometries that belong to realm non‐Euclidean geometry and remain a challenge for traditional engineering approaches. Here, it is shown how simple deflection thick meta‐plates based on hexagonal cellular mesostructures can be used achieve wide range intrinsic (i.e., Gaussian) curvatures, including dome‐like saddle‐like shapes. Depending unit cell structure, non‐auxetic positive Poisson ratio) or...

The rational design of bone-substituting biomaterials is relatively complex because they should meet a long list requirements for optimal performance. Meta-biomaterials are micro-architected materials that hold great promise meeting those as offer unique combination mechanical, mass-transport, and biological properties. There are, however, inherent couplings between the different types properties many such make it impossible to simultaneously achieve all criteria. An example coupling exists...

3D organoids are widely used as tractable in vitro models capable of elucidating aspects human development and disease. However, the manual low-throughput culture methods, coupled with a low reproducibility geometric heterogeneity, restrict scope application organoid research. Combining expertise from stem cell biology bioengineering offers promising approach to address some these limitations. Here, melt electrospinning writing is generate tuneable grid scaffolds that can guide...

Origami-inspired folding methods present novel pathways to fabricate three-dimensional (3D) structures from 2D sheets. A key advantage of this approach is that planar printing and patterning processes could be used prior folding, affording enhanced surface functionality the folded structures. This particularly useful for 3D lattices, possessing very large internal areas. While polyhedral strut-based lattices has already been demonstrated, more complex, curved sheet-based have not yet due...

Abstract Emerging 4D printing techniques have enabled the realization of smart materials whose shape or properties can change with time. Two important phenomena play roles in memory polymeric materials. First, anisotropic deformation printed filaments due to residual stresses be harnessed create out-of-plane transformations. Second, unavoidable formation micro-defects during processes often affects programmability object. Here, we propose a design approach that harnesses these two effects...

Abstract Fracture non‐union occurs due to various factors, leading the development of potentially substantial bone defects. While autograft and allograft are current gold standards for fractures, challenges related availability immune rejection highlight need improved treatments. A strategy in tissue engineering is harness growth factors induce an effect on cells change their phenotype, behavior initiate signaling pathways which lead increased matrix deposition formation. Bone morphogenetic...

Biomaterial-based approaches for bone regeneration seek to explore alternative strategies repair non-healing fractures and critical-sized defects. Fracture non-union occurs due a number of factors resulting in the formation Rigorous evaluation biomaterials relevant models assessment their potential translate towards clinical use is vital. Large animal experimentation can be used model fracture while scaling-up materials use. Growth modulate cell phenotype, behaviour initiate signalling...

Abstract Fracture non-union occurs as a consequence of various factors, leading to the development potentially substantial bone defects. Biomaterial-based approaches for regeneration aim explore alternative strategies repair non-healing fractures and critical-sized Thus, rigorous assessment ability translate biomaterials towards clinical use is vital. Growth factors induce an effect on cells change their phenotype, behaviour initiate signalling pathways, matrix deposition tissue formation....

A bstract Individual cells and multicellular systems have been shown to respond cell-scale curvatures in their environments, guiding migration, orientation, tissue formation. However, it remains unclear how collectively explore pattern complex landscapes with curvature gradients across the Euclidean non-Euclidean spectra, partly owing fabrication limitations lack of formal geometric considerations. Here, we show that micro-engineered substrates controlled variations induce collective...

Abstract Bone tissue engineering is a rapidly advancing field that seeks to develop efficacious approaches for treating non-healing fractures and large bone defects. Healing complications arise due trauma, disease, infection, aseptic loosening of orthopaedic implants or iatrogenic causes. An ideal biodegradable scaffold would induce support formation until the matrix sufficiently stable facilitate healing. The current study has examined augmentation, using functionalised coated scaffolds,...

Cells typically respond to a variety of geometrical cues in their environment, ranging from nanoscale surface topography mesoscale curvature. The ability control cellular organisation and fate by engineering the shape extracellular milieu offers exciting opportunities within tissue engineering. Despite great progress, however, many questions regarding geometry-driven growth remain unanswered. Here, we combine mathematical design, high-resolution microfabrication, vitro cell culture,...

Abstract Biomaterial-based approaches for bone regeneration seek to explore alternative strategies repair non-healing fractures and critical-sized defects. Fracture non-union occurs due a number of factors resulting in the formation Rigorous evaluation biomaterials relevant models assessment their potential translate towards clinical use is vital. Large animal experimentation can be used model fracture while scaling-up materials use. Growth modulate cell phenotype, behaviour initiate...

Abstract Bone tissue engineering seeks to develop treatment approaches for nonhealing and large bone defects. An ideal biodegradable scaffold will induce support formation. The current study examines augmentation in critical‐sized defects, using functionalized scaffolds, with the hypothesized potential skeletal cell differentiation. 3D printed, porous poly(caprolactone) trimethacrylate (PCL‐TMA900) scaffolds are applied within a murine femur defect, stabilized by polyimide intramedullary...

A bstract The organization and shape of the microstructural elements trabecular bone govern its physical properties, are implicated in disease, can serve as blueprints for biomaterial design. To devise fundamental structure-property relationships, it is essential to characterize from perspective geometry, mathematical study shape. Here, we used micro-computed tomography images 70 donors at five different sites local global geometry human bone, respectively quantified by surface curvatures...

In article number 2101373, Sebastien J. P. Callens and co-workers describe a novel parametric approach to designing biphasic metamaterials based on minimal surfaces, which is demonstrated using multi-material 3D printing. This enables independent tuning of the mechanical mass transport properties, feature that highly relevant in multi-physics applications, such as metabiomaterials for tissue engineering.