Journal Article Comparison of traditional and molecular analytical methods for detecting biological agents in raw drinking water following ultrafiltration Get access D.S. Francy, Francy U.S. Geological Survey, Ohio Water Science Center, Columbus, OH, USA Donna S. 6480 Doubletree Ave., OH 43229, USA. E‐mail: dsfrancy@usgs.gov Search other works by this author on: Oxford Academic Google Scholar R.N. Bushon, Bushon A.M.G. Brady, Brady E.E. Bertke, Bertke C.M. Kephart, Kephart C.A. Likirdopulos,...

The aim of this study was to evaluate the variability in lots a DNA extraction kit using real-time PCR assays for Bacillus anthracis, Francisella tularensis and Vibrio cholerae.Replicate aliquots three bacteria were processed duplicate with different commercial kit. This experiment repeated triplicate. Results showed that cycle threshold values statistically among lots.Differences reagent found be significant source qPCR results. Steps should taken ensure quality consistency reagents....

Source-tracking tools were used to identify potential sources of fecal contamination at two Lake Erie bathing beaches: an urban beach (Edgewater in Cleveland, Ohio) and a small city (Lakeshore Ashtabula, Ohio). These included identifying spatial patterns Escherichia coli (E. coli) concentrations each area, determining weather that caused elevated E. coli, applying microbial source tracking (MST) techniques specific sites. Three MST methods during this study: multiple antibiotic resistance...

First posted September 9, 2020 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180–8349 The U.S. Survey, in cooperation with the Village of East Hampton, York, conducted a 1-year study from August 2017 to 2018 provide data necessary improve understanding sources nutrients and pathogens Hook Pond watershed allow for possible mitigation or reduction loads. Chronic eutrophication recent concern over harmful...

First posted May 27, 2021 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 Between June 2018 and July 2019, the U.S. Survey collaborated with State Department of Environmental Conservation to analyze water quality in seven embayments on Long Island, York, for a study examine fecal contamination using microbial source tracking. This report documents approach, methodology, quality-assurance data used study. All...

Abstract A rapid method, quantitative polymerase chain reaction (qPCR), for quantifying indicator bacteria in recreational waters is desirable public health protection. We report that replacing current Escherichia coli standards with new US Environmental Protection Agency beach action values (BAVs) enterococci by culture or qPCR may result more advisories being posted at inland lakes. In this study, concentrations of E. and methods were compared to Enterococcus spp. 3 lake beaches Ohio. The...

During the recreational seasons of 2006 and 2007, quantitative polymerase chain reaction (qPCR) method was used to determine Escherichia coli (E. coli) concentrations in samples from two Lake Erie beaches. Results qPCR were compared those obtained by traditional culturing on modified mTEC agar. Regression analysis showed strong, statistically significant correlations between results methods for both years. Correlation coefficients at Edgewater Villa Angela Beaches 0.626 0.789 0.667 0.829...

First posted March 24, 2023 For additional information, contact: Director, New Mexico Water Science Center U.S. Geological Survey 6700 Edith Blvd. NE Albuquerque, NM 87113 Contact Pubs Warehouse The Rio Grande, in southern Mexico, is a Clean Act Section 303(d) Category 5 impaired reach for Escherichia coli (E. coli). miles length, extending from Tijeras Arroyo south to the Isleta Pueblo boundary. An evaluation of E. and microbial source tracking markers (human-, canine-, waterfowl-specific...

The U.S. Geological Survey (USGS), in collaboration with the Louisiana Department of Environmental Quality, monitored bacteriological quality water at 22 sites and around Lake Pontchartrain, La., for three consecutive weeks beginning September 13, 2005, following hurricanes Katrina Rita associated flooding. Samples were collected analyzed by USGS personnel from Water Science Center Ohio Microbiology Laboratory. Fecal-indicator bacteria (Escherichia coli, enterococci, fecal coliform)...

To reduce the impact from a possible bioterrorist attack on drinking-water supplies, analytical methods are needed to rapidly detect presence of biological agents in water. this end, 13 samples were collected at 9 water-treatment plants Ohio assess performance molecular method comparison traditional that take longer perform. Two 100-liter each site during sampling event; one was seeded laboratory with six - Bacillus anthracis (B. anthracis), Burkholderia cepacia (as surrogate for Bu....

First posted March 5, 2018 For additional information, contact: Director,Arizona Water Science CenterU.S. Geological Survey520 N. Park AvenueTucson, AZ 85719 Tumacácori National Historical protects the culturally important Mission, San José de Tumacácori, while also managing a portion of ecologically diverse riparian corridor Santa Cruz River. This report describes methods and quality assurance procedures used in collection water samples for analysis Escherichia coli (E. coli), microbial...

First posted October 15, 2019 For additional information, contact: Director,Arizona Water Science CenterU.S. Geological Survey520 N. Park AvenueTucson, AZ 85719 Tumacácori National Historical (TUMA) in southern Arizona protects the culturally important Mission San José de Tumacácori, while also managing a part of ecologically diverse riparian corridor Santa Cruz River. The quality water flowing through depends solely on upstream watershed activities, and among water-quality issues concerning...

An influx of concentrated animal feeding operations in northwest Ohio has prompted local agencies to examine the effects these industrial farms on water quality upper Portage River watershed. The utility microbial source-tracking (MST) tools as a means characterizing sources fecal contamination watershed was evaluated. From 2007 2008, scientists with U.S. Geological Survey, Bowling Green State University, and Wood County Health Department collected analyzed 17 environmental samples 13 source...

First posted August 9, 2021 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180–8349 The U.S. Survey worked collaboratively with the State Department of Environmental Conservation to assess potential sources fecal contamination entering Hempstead Harbor, an embayment on northern shore Nassau County, Long Island, York. samples are routinely collected by in harbor and analyzed for coliform bacteria, indicator...

First posted February 26, 2019 For additional information, contact: Director, Ohio-Kentucky-Indiana Water Science CenterU.S. Geological Survey6460 Busch Blvd, Suite 100Columbus, OH 43229 Elevated Escherichia coli (E. coli) concentrations at the Maumee Bay State Park (MBSP) Lake Erie beach have resulted in frequent recreational water-quality advisories. After construction of a wetland along Berger Ditch Park, Oregon, Ohio, samples were collected and analyzed for E. microbial source tracking...

First posted January 18, 2022 For additional information, contact: Director, Upper Midwest Water Science Center U.S. Geological Survey5840 Enterprise Drive Lansing, MI 48911Contact Pubs Warehouse Lake St. Clair Metropark Beach (LSCMB) in Michigan is a public beach near the mouth of Clinton River that has history closures for health concerns. The designated as Great Lakes Area Concern, and park Beneficial Use Impairment closings because elevated Escherichia coli (E. coli) concentrations....

First posted April 5, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 The U.S. Survey worked in cooperation with the State Department of Environmental Conservation to assess potential sources fecal contamination entering Sag Harbor, an embayment complex on northern shore south fork Suffolk County, Long Island, York. samples are routinely collected by harbor and analyzed for coliform bacteria, indicator...

First posted March 30, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 The U.S. Survey worked in cooperation with the State Department of Environmental Conservation to assess potential sources fecal contamination entering Patchogue and Bellport Bays, two embayments on south shore Suffolk County, Long Island, York. samples are routinely collected by bays analyzed for coliform bacteria, an indicator...

First posted April 15, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 The U.S. Survey worked in cooperation with the Concerned Citizens of Montauk and State Department Environmental Conservation to assess potential sources fecal contamination entering Lake Montauk, an artificial embayment on tip southern fork Suffolk County, Long Island, York. samples are routinely collected by harbor analyzed for...

First posted March 10, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 The U.S. Survey worked in cooperation with the State Department of Environmental Conservation to assess potential sources fecal contamination entering Port Jefferson Harbor, Setauket and Conscience Bay, an embayment complex on northern shore Suffolk County, Long Island, York. samples are routinely collected by harbor analyzed for...

First posted June 23, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180-8349 The U.S. Survey worked in cooperation with the State Department of Environmental Conservation to assess potential sources fecal contamination entering a part Great South Bay (referred as for purposes this report) near hamlets West Sayville, and Bayport on southern shore Suffolk County Long Island, York. samples are routinely collected...

First posted July 20, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180–8349 Alley Creek, a tributary to Little Neck Bay in Queens County, York, has been designated by the State Department of Environmental Conservation as impaired (Class I) for fecal coliform because pollution from combined sewer overflow, including stormwater runoff. The U.S. Survey, cooperation with City Protection, conducted 1-year study...

First posted August 11, 2022 For additional information, contact: Director, New York Water Science CenterU.S. Geological Survey425 Jordan RoadTroy, NY 12180–8349 The U.S. Survey worked in cooperation with the State Department of Environmental Conservation to assess potential sources fecal contamination entering South Oyster Bay, a shallow embayment on southern shore Long Island, York. samples are routinely collected by bay and analyzed for coliform bacteria, an indicator contamination,...