A5011997016- Fecal contamination and water quality
- Water Treatment and Disinfection
- Water Quality and Resources Studies
- Viral gastroenteritis research and epidemiology
- Urban Stormwater Management Solutions
- Mycobacterium research and diagnosis
- Bacillus and Francisella bacterial research
- Vibrio bacteria research studies
- Environmental Justice and Health Disparities
- Molecular Biology Techniques and Applications
- Child Nutrition and Water Access
- Coal and Its By-products
- Hydrology and Sediment Transport Processes
- Biosensors and Analytical Detection
- Groundwater flow and contamination studies
- Fish Ecology and Management Studies
- Aquaculture disease management and microbiota
- Wastewater Treatment and Reuse
- Bacteriophages and microbial interactions
- Flood Risk Assessment and Management
- Landslides and related hazards
- Soil and Water Nutrient Dynamics
- Hepatitis Viruses Studies and Epidemiology
- Tailings Management and Properties
- Tropical and Extratropical Cyclones Research
United States Geological Survey
2006-2021
Ohio-Kentucky-Indiana Water Science Center
2021
Indiana Geological and Water Survey
2021
Great Lakes Science Center
2013-2017
United States Department of the Interior
2007
National Wildlife Health Center
2007
ABSTRACT Bacterial indicators are used to indicate increased health risk from pathogens and make beach closure advisory decisions; however, beaches seldom monitored for the themselves. Studies of sources types at needed improve estimates swimming-associated risks. It would be advantageous cost-effective, especially studies conducted on a regional scale, use method that can simultaneously filter concentrate all classes large volumes water detect pathogens. In seven recovery experiments, stock...
The application of quantitative real-time PCR (qPCR) technologies for the rapid identification fecal bacteria in environmental waters is being considered use as a national water quality metric United States. transition from research tool to standardized protocol requires information on reproducibility and sources variation associated with qPCR methodology across laboratories. This study examines interlaboratory variability measurement enterococci Bacteroidales concentrations standardized,...
Journal Article Comparison of traditional and molecular analytical methods for detecting biological agents in raw drinking water following ultrafiltration Get access D.S. Francy, Francy U.S. Geological Survey, Ohio Water Science Center, Columbus, OH, USA Donna S. 6480 Doubletree Ave., OH 43229, USA. E‐mail: dsfrancy@usgs.gov Search other works by this author on: Oxford Academic Google Scholar R.N. Bushon, Bushon A.M.G. Brady, Brady E.E. Bertke, Bertke C.M. Kephart, Kephart C.A. Likirdopulos,...
The aim of this study was to examine a rapid method for detecting Escherichia coli and enterococci in recreational water.Water samples were assayed E. by traditional immunomagnetic separation/adenosine triphosphate (IMS/ATP) methods. Three sample treatments evaluated the IMS/ATP method: double filtration, single direct analysis. Pearson's correlation analysis showed strong, significant, linear relations between methods all treatments; strongest correlations with (r = 0.62 0.77 enterococci,...
Membrane bioreactors (MBR), used for wastewater treatment in Ohio and elsewhere the United States, have pore sizes small enough to theoretically reduce concentrations of protozoa bacteria, but not viruses. Sampling viruses is seldom done required. Instead, bacterial indicators Escherichia coli (E. coli) fecal coliforms are required microbial measures effluents wastewater-discharge permits. Information needed on effectiveness MBRs removing human enteric from wastewaters, particularly as...
The aim of this study was to evaluate the variability in lots a DNA extraction kit using real-time PCR assays for Bacillus anthracis, Francisella tularensis and Vibrio cholerae.Replicate aliquots three bacteria were processed duplicate with different commercial kit. This experiment repeated triplicate. Results showed that cycle threshold values statistically among lots.Differences reagent found be significant source qPCR results. Steps should taken ensure quality consistency reagents....
Microbiological and hydrological data were used to rank tributary stream contributions of bacteria the Little Blue River in Independence, Missouri. Concentrations, loadings yields E. coli microbial source tracking (MST) markers, characterized during base flow storm events five subbasins within as well sources entering leaving city through river. The water quality threshold was exceeded 29% base-flow 89% storm-event samples. total contribution MST markers from tributaries Independence River,...
Abstract A rapid method, quantitative polymerase chain reaction (qPCR), for quantifying indicator bacteria in recreational waters is desirable public health protection. We report that replacing current Escherichia coli standards with new US Environmental Protection Agency beach action values (BAVs) enterococci by culture or qPCR may result more advisories being posted at inland lakes. In this study, concentrations of E. and methods were compared to Enterococcus spp. 3 lake beaches Ohio. The...
A study of small public ground-water-supply wells that produce water from discontinuous sand and gravel aquifers was done July 1999 through 2001 in southeastern Michigan. Samples were collected to determine the occurrence viral pathogens microbiological indicators fecal contamination (indicators), whether are adequate predictors presence enteric viruses, factors affect viruses. Small systems those serve less than 3,300 people. analyzed for specific viruses by reverse transcriptase-polymerase...
During the recreational seasons of 2006 and 2007, quantitative polymerase chain reaction (qPCR) method was used to determine Escherichia coli (E. coli) concentrations in samples from two Lake Erie beaches. Results qPCR were compared those obtained by traditional culturing on modified mTEC agar. Regression analysis showed strong, statistically significant correlations between results methods for both years. Correlation coefficients at Edgewater Villa Angela Beaches 0.626 0.789 0.667 0.829...
The Cuyahoga River within Valley National Park (CVNP) in Ohio is often impaired for recreational use because of elevated concentrations bacteria, which are indicators fecal contamination. During the seasons (May through August) 2004 2007, samples were collected at two river sites, one upstream and centrally-located CVNP. Bacterial turbidity determined, streamflow time sampling rainfall amounts over previous 24 hours prior to ascertained. Statistical models predict Escherichia coli (E. coli)...
The U.S. Geological Survey (USGS), in collaboration with the Louisiana Department of Environmental Quality, monitored bacteriological quality water at 22 sites and around Lake Pontchartrain, La., for three consecutive weeks beginning September 13, 2005, following hurricanes Katrina Rita associated flooding. Samples were collected analyzed by USGS personnel from Water Science Center Ohio Microbiology Laboratory. Fecal-indicator bacteria (Escherichia coli, enterococci, fecal coliform)...
To reduce the impact from a possible bioterrorist attack on drinking-water supplies, analytical methods are needed to rapidly detect presence of biological agents in water. this end, 13 samples were collected at 9 water-treatment plants Ohio assess performance molecular method comparison traditional that take longer perform. Two 100-liter each site during sampling event; one was seeded laboratory with six - Bacillus anthracis (B. anthracis), Burkholderia cepacia (as surrogate for Bu....
In May 2002, 15 wells and four surface water sites were sampled, in September those same plus additional sampled five parishes of northwestern Louisiana. A geographic information system (GIS) was used to select residential for sampling. Well samples analyzed pH, conductivity, organic compounds, nutrient anion concentrations. All further tested presence fungi (maintained up 28 days colonies counted identified microscopically), metal trace element concentration by inductively-coupled plasma...
An influx of concentrated animal feeding operations in northwest Ohio has prompted local agencies to examine the effects these industrial farms on water quality upper Portage River watershed. The utility microbial source-tracking (MST) tools as a means characterizing sources fecal contamination watershed was evaluated. From 2007 2008, scientists with U.S. Geological Survey, Bowling Green State University, and Wood County Health Department collected analyzed 17 environmental samples 13 source...
Following the Louisiana landfalls of Katrina on August 29 and Rita September 24, 2005, local population American public were concerned about effects hurricanes might have water quality in Lake Pontchartrain. The lake is a major recreational resource for region an important fishery. Contamination carried by storm surge—along with runoff pumped from flooded areas New Orleans—was considered serious threat to body. USGS, collaboration LDEQ, monitored sanitary at 22 sites around Pontchartrain,...
During the 2007 recreational season at Villa Angela Beach in Cleveland, Ohio, scientists with U.S. Geological Survey (USGS) and Northeast Ohio Regional Sewer District (NEORSD) found high Escherichia coli (E. coli) concentrations that were not easily explained by results obtained to date ongoing investigations of water quality beach. To help understand sources behind these elevated E. concentrations, USGS NEORSD sampled beach-area for Bacteroides DNA markers. are a group enteric bacteria...