A5102024107- Connective Tissue Growth Factor Research
- Cardiac Fibrosis and Remodeling
- Signaling Pathways in Disease
- GDF15 and Related Biomarkers
- Pain Management and Treatment
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Cardiovascular Effects of Exercise
- Biomarkers in Disease Mechanisms
- Chemokine receptors and signaling
- Cardiovascular and exercise physiology
- Systemic Sclerosis and Related Diseases
- Sirtuins and Resveratrol in Medicine
- PARP inhibition in cancer therapy
- Pulmonary Hypertension Research and Treatments
- Viral Infections and Outbreaks Research
- Connective tissue disorders research
- Frailty in Older Adults
- Tissue Engineering and Regenerative Medicine
- Neuropeptides and Animal Physiology
- Liver physiology and pathology
- Poxvirus research and outbreaks
- Calpain Protease Function and Regulation
- Cardiac Structural Anomalies and Repair
- Nutrition and Health in Aging
Majmaah University
2023-2024
Oslo University Hospital
2009-2022
University of Oslo
2009-2022
CCN2/connective tissue growth factor (CTGF), a CCN family matricellular protein repressed in healthy hearts after birth, is induced heart failure of various etiologies. Multiple cellular and biological functions have been assigned to CCN2/CTGF depending on context. However, the mechanisms action as well its roles cardiac physiology pathophysiology remain unknown. Transgenic mice with cardiac-restricted overexpression CTGF (Tg-CTGF) were generated compared nontransgenic littermate control...
Aims Myocardial CCN2/CTGF is induced in heart failure of various etiologies. However, its role the pathophysiology left ventricular (LV) remodeling after myocardial infarction (MI) remains unresolved. The current study explores CTGF infarct healing and LV an animal model patients admitted for acute ST-elevation MI. Methods Results Transgenic mice with cardiac-restricted overexpression (Tg-CTGF) non-transgenic littermate controls (NLC) were subjected to permanent ligation anterior descending...
Although exercise training has been demonstrated to have beneficial cardiovascular effects in diabetes, the effect of on hearts from obese/diabetic models is unclear. In present study, mice were fed a high-fat diet, which led obesity, reduced aerobic capacity, development mild diastolic dysfunction, and impaired glucose tolerance. Following 8 wk assigned 5 weekly high-intensity interval (HIT) sessions (10 × 4 min at 85–90% maximum oxygen uptake) or remained sedentary for next 10 constitutive...
NAD+ is an essential cofactor in reduction-oxidation metabolism with impact on metabolic and inflammatory diseases. However, data elucidating the effects of proinflammatory features human primary monocytes are scarce. In this study, we explored how affects TLR4 NOD-like receptor a PYD-domain 3 (NLRP3) inflammasome activation, two key innate immune responses. Human were isolated from buffy coats obtained healthy individuals. Intracellular was manipulated by nicotinamide riboside NAMPT...
Glioblastoma (GBM) is the most common primary brain malignancy and confers a dismal prognosis. GBMs harbor glioblastoma-initiating cells (GICs) that drive tumorigenesis contribute to therapeutic resistance tumor recurrence. Consequently, there strong rationale target this cell population in order develop new molecular therapies against GBM. Accumulating evidence indicates Nα-terminal acetyltransferases (NATs), are dysregulated numerous human cancers, can serve as targets. Microarrays were...
Poly(ADP-ribose) polymerase (PARP) enzymes initiate (mt)DNA repair mechanisms and use nicotinamide adenine dinucleotide (NAD + ) as energy source. Prolonged PARP activity can drain cellular NAD reserves, leading to de-regulation of important molecular processes. Here, we provide evidence a pathophysiological mechanism that connects mtDNA damage cardiac dysfunction via reduced levels loss mitochondrial function communication. Using transgenic model, demonstrate high mice cardiomyocyte cause...
AimsAortic stenosis induces pressure overload and myocardial remodelling with concentric hypertrophy alterations in extracellular matrix (ECM). Aortic valve replacement leads to reverse remodelling, a process of which knowledge is scarce. The aims the present study were examine gene expression subsequently identify molecular important for early phase remodelling.
Rationale Inflammatory mechanisms have been suggested to play a role in the development of heart failure (HF), but for chemokines is largely unknown. Based on their inflammation and matrix remodeling other tissues, we hypothesized that CXCL13 CXCR5 could be involved cardiac during HF. Objective We sought analyze chemokine its receptor pathophysiology leading Methods Results Mice harboring systemic knockout (CXCR5−/−) displayed increased mortality follow-up 80 days after aortic banding (AB)....
Background CCL21 acting through CCR7, is termed a homeostatic chemokine. Based on its role in concerting immunological responses and proposed involvement tissue remodeling, we hypothesized that this chemokine could play myocardial remodeling during left ventricular (LV) pressure overload. Methods Results Our main findings were: (i) Serum levels of were markedly raised patients with symptomatic aortic stenosis (AS, n = 136) as compared healthy controls (n 20). (ii) A level the highest tertile...
Abstract Aims: Connective tissue growth factor (CTGF) is a secreted, heparin‐binding, and extracellular matrix associated protein shown to stimulate many of the cellular events underlying fibrosis. Previous investigations have revealed that myocardial CTGF substantially induced in ischaemic heart failure, particularly peri‐ischaemic region. The purpose present study was investigate what extent induction general response congestive failure (CHF) decisive effector Methods: Experimental pigs by...
Cardiomyocyte hypertrophy is a hallmark of cardiac dysfunction in patients with aortic stenosis (AS), and can be triggered by left ventricular (LV) pressure overload mice banding (AB). Syndecan-4 transmembrane heparan sulphate proteoglycan which found increased the myocardium AS AB mice. The role syndecan-4 cardiomyocyte not well understood.We developed cardiomyocyte-specific overexpression (Sdc4-Tg) subjected these to examine activation pro-hypertrophic calcineurin-NFAT signalling...
Transgenic mice with cardiac-specific expression of a peptide inhibitor G protein-coupled receptor kinase (GRK)3 [transgenic COOH-terminal GRK3 (GRK3ct) mice] display myocardial hypercontractility without hypertrophy and enhanced α(1)-adrenergic signaling. A role for in the pathogenesis heart failure (HF) has not been investigated, but inhibition its isozyme, GRK2, beneficial several HF models. Here, we tested whether modulated evolving cardiac dysfunction after pressure overload....
Myocardial connective tissue growth factor (CTGF/CCN2) is induced in heart failure, a condition associated with diminution of <i>β</i>-adrenergic receptor (<i>β</i>-AR) responsiveness. Accordingly, we aimed to investigate whether CTGF could play mechanistic role regulation <i>β</i>-AR Concentration-response curves isoproterenol-stimulated cAMP generation cardiomyocytes from transgenic mice cardiac-restricted overexpression (Tg-CTGF) or pretreated recombinant human (rec-hCTGF) revealed marked...
CCN5 is a divergent member of the cellular communication network factor (CCN) family in that it lacks carboxyl terminal cystine knot domain common to other CCN members. has been reported antagonize profibrotic actions CCN2 and inhibit myocardial collagen deposition fibrosis chronic pressure overload heart. However, what mechanisms regulate activity heart remain unknown. Recombinant, replication defective adenovirus encoding firefly luciferase under control human promoter was prepared used...
Abstract Accurate evaluation of liver steatosis is required from brain-dead donors (BDDs) with nonalcoholic fatty disease (NAFLD). Our purposes were to investigate expression and regulation connective tissue growth factor (CTGF) in livers human rat after brain death, further evaluate its potential application. NAFLD death models established rats. LX2 cells cultured under hypoxia/reoxygenation. CTGF protein mRNA levels measured samples BDDs by immunohistochemistry reverse...
Abstract Background The recent Marburg virus outbreaks in Equatorial Guinea and Tanzania March 2023 have sparked fears about potential worldwide spread. Thus, assessing healthcare workers’ (HCW) knowledge of the disease is critical, particularly India, where system underfunded understaffed already has an immense burden infectious illnesses. Methods We carried out a cross-sectional web-based survey among 300 workers including doctors, nurses, pharmacists. Google form was circulated online via...
Aims Patients with rheumatoid arthritis (RA) have increased risk of heart failure (HF). The mechanisms and cardiac prerequisites explaining this association remain unresolved. In study, we sought to determine the potential impact an experimental model RA in mice subjected HF by constriction ascending aorta. Methods Aorta was constricted via thoracotomy placement o-rings inner diameter 0.55 mm or 0.66 mm, sham operated. RA-like phenotype instigated delayed-type hypersensitivity (DTHA) two...