A5074391361- Plant tissue culture and regeneration
- Nanoparticles: synthesis and applications
- Plant Molecular Biology Research
- Transgenic Plants and Applications
- Extracellular vesicles in disease
- Plant Stress Responses and Tolerance
- Circular RNAs in diseases
- RNA Interference and Gene Delivery
- Heavy Metal Exposure and Toxicity
- Plant-Microbe Interactions and Immunity
- Graphene and Nanomaterials Applications
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Photosynthetic Processes and Mechanisms
- Essential Oils and Antimicrobial Activity
- Circadian rhythm and melatonin
- Microbial Metabolic Engineering and Bioproduction
- Redox biology and oxidative stress
- Medicinal Plants and Neuroprotection
- Pineapple and bromelain studies
- Nanoparticle-Based Drug Delivery
- Plant nutrient uptake and metabolism
- Adipose Tissue and Metabolism
- MicroRNA in disease regulation
Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RAS
2021-2025
Exosomal nanoparticles (exosomes or nanovesicles) are biogenic membrane vesicles secreted by various cell types and represent a conservative mechanism of intercellular interspecies communication in pro- eukaryotic organisms. By transporting specific proteins, nucleic acids, low molecular weight metabolites, the exosomes involved regulation developmental processes, activation immune system, development protective response to stress. Recently, plant nanovesicles, due an economical affordable...
The present study reports a green chemistry approach for the rapid and easy biological synthesis of silver (Ag), gold (Au), bimetallic Ag/Au nanoparticles using callus extract Lithospermum erythrorhizon as reducing capping agent. biosynthesized were characterized with ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD) analysis, transmission electron microscopy (TEM). Our results showed formation crystalline metal nanostructures both spherical non-spherical shape. Energy...
Over recent years, extracellular vesicles (EVs), commonly termed exosomes, have gained prominence for their potential as natural nanocarriers. It has now been recognized that plants also secrete EVs. Despite this discovery, knowledge about EV biogenesis in plant cell cultures remains limited. In our study, we isolated and meticulously characterized EVs from the callus culture of model plant,
Background: Recent interest in plant-derived exosome-like nanoparticles (ENs) has surged due to their therapeutic potential, which includes antioxidant, anti-inflammatory, and anticancer activities. These properties are attributed cargo of bioactive metabolites other endogenous molecules. However, the ENs isolated from plant cell cultures remain less explored. Methods: In this investigation, grape callus-derived (GCENs) were using differential ultracentrifugation techniques. Structural...
This study delves into the novel utilization of Aristolochia manshuriensis cultured cells for extracellular silver nanoparticles (AgNPs) synthesis without need additional substances. The presence elemental has been verified using energy-dispersive X-ray spectroscopy, while distinct surface plasmon resonance peaks were revealed by UV-Vis spectra. Transmission and scanning electron microscopy indicated that AgNPs, ranging in size from 10 to 40 nm, exhibited a spherical morphology....
The E3 ubiquitin-protein ligase HOS1 is an important integrator of temperature information and developmental processes. a negative regulator plant cold tolerance, silencing leads to increased tolerance. In the present work, we studied ROS levels in hos1Cas9 Arabidopsis thaliana plants, which gene was silenced by disruption open reading frame via CRISPR/Cas9 technology. Confocal imaging intracellular reactive oxygen species (ROS) showed that hos1 mutation moderately under both low high light...
An efficient Agrobacterium-mediated genetic transformation based on the plant binary vector pPZP-RCS2 was carried out for multiple heterologous protein production in filamentous fungus Thermothelomyces thermophilus F-859 (formerly Myceliophthora thermophila F-859). The engineered Th. thermophilus able to produce storage proteins of Zea mays (α-zein Z19) and Amaranthus hypochondriacus (albumin A1) enrich fungal biomass by valuable nutritional improved amino acid...