A5020134297- Cellular Mechanics and Interactions
- Invertebrate Immune Response Mechanisms
- Marine Bivalve and Aquaculture Studies
- Glioma Diagnosis and Treatment
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- RNA Interference and Gene Delivery
- Heavy Metal Exposure and Toxicity
- Trace Elements in Health
- Aquatic Invertebrate Ecology and Behavior
- Force Microscopy Techniques and Applications
- Advanced Drug Delivery Systems
- Hydrogels: synthesis, properties, applications
- Advanced biosensing and bioanalysis techniques
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- Aluminum toxicity and tolerance in plants and animals
- Parasite Biology and Host Interactions
- Nanoparticles: synthesis and applications
- Environmental Toxicology and Ecotoxicology
- Tissue Engineering and Regenerative Medicine
- Marine Sponges and Natural Products
- Adipose Tissue and Metabolism
A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences
2011-2025
Far Eastern Federal University
2015-2025
Far Eastern Branch of the Russian Academy of Sciences
2016-2024
Russian Academy of Sciences
2011-2019
Wilson's disease (WD) is an autosomal recessive disorder characterized by toxic accumulation of copper in the liver, brain, and other organs. The caused pathogenic variants ATP7B gene, which encodes a P-type transport ATPase. Diagnosing WD associated with numerous difficulties due to wide range clinical manifestations its unknown dependence on physiological characteristics patient. This leads delay start therapy subsequent deterioration patient's condition. However, recent years, molecular...
In this study, a calcium-phosphate coating was formed on Mg–Mn–Ce alloy by the plasma electrolytic oxidation (PEO). The antibiotic vancomycin, widely used in treatment of infections caused Staphylococcus aureus (S. aureus), impregnated into coating. Samples with vancomycin showed high bactericidal activity against S. aureus. mechanical and electrochemical properties coatings were studied, as well vitro cytotoxicity tests vivo mature male rats performed. According to SEM, EDS, XRD XPS data,...
Bivalves play a key role in aquatic ecosystems and are valuable commercial resource. The prosperity of these organisms depends mainly on the effectiveness their immune defense, which hemolymph plays central role. Hemolymph may be used as an effective non-lethal criterion health. However, predictive value analysis comparison between obtained results reference data from healthy individuals living natural environments. We collected 15 commercially important species wild populations at stations...
The present study reports a green chemistry approach for the rapid and easy biological synthesis of silver (Ag), gold (Au), bimetallic Ag/Au nanoparticles using callus extract Lithospermum erythrorhizon as reducing capping agent. biosynthesized were characterized with ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD) analysis, transmission electron microscopy (TEM). Our results showed formation crystalline metal nanostructures both spherical non-spherical shape. Energy...
// Jung-Hyun Kim 1 , Hee-Sheung Lee Nicholas C. O. Nikolay V. Goncharov 1,2 Vadim Kumeiko 2 Hiroshi Masumoto 3 William Earnshaw 4 Natalay Kouprina and Vladimir Larionov Developmental Therapeutics Branch, National Cancer Institute, NIH, Bethesda, MD, USA School of Biomedicine, Far Eastern Federal University, A. Zhirmunsky Institute Marine Biology, FEB RAS, Vladivostok, Russia Laboratory Cell Engineering, Department Human Genome Research, Kazusa DNA Research Kisarazu, Japan Wellcome Trust...
Abstract Glioma is a devastating brain tumor with high mortality rate attributed to the glioma stem cells (GSCs) possessing plasticity. Marker mutations in isocitrate dehydrogenase type 1 ( IDH1 ) and protein 53 TP53 are frequent gliomas impact cell fate decisions. Understanding GSC heterogeneity within IDH1- TP53- mutant tumors may elucidate possible treatment targets. Here, we performed single-nucleus transcriptomics of wild-type samples sorted for Sox2 marker. For first time rare...
A small protein, Mitoregulin (Mtln), localizes in mitochondria and contributes to oxidative phosphorylation fatty acid metabolism. Mtln knockout mice develop obesity on a high-fat diet, demonstrating elevated cardiolipin damage suboptimal creatine kinase oligomerization muscle tissue. Kidneys heavily depend the mitochondria. Here we report kidney-related phenotypes aged mice. Similar mitochondria, those of kidney demonstrate decreased respiratory complex I activity excessive damage. Aged...
Background: Chronic inflammation is an important factor in the development and progression of colorectal cancer (CRC). One key participants chronic interleukin-6 (IL-6), which enhances tumor malignancy. Some genetic markers characterizing IL-6/STAT3/JAK pathway are functional polymorphisms IL6 IL6R genes. Hyperexpression IL-6 increasing concentrations soluble form, IL-6R, may be one possible option for cross-activation MAPK signaling. Methods: Detection rs1800795 rs2228145 SNPs was carried...
Recently revised brain tumor classification suggested a glioma treatment strategy that takes into consideration molecular variants in IDH1 and TP53 marker genes. While pathogenic of can be accompanied by chromosomal instability (CIN), the impact mutations on genome stability remains unstudied. Elevated CIN might provide therapeutic targets, based synergistic effects chemotherapy with CIN-inducing drugs. Using an assay human artificial chromosomes, we investigated common missense chromosome...
Aristolochia manshuriensis is a relic liana, which widely used in traditional Chinese herbal medicine and endemic to the Manchurian floristic region. Since this plant rare slow-growing, alternative sources of its valuable compounds could be explored. Herein, we established hairy root cultures A. transformed with Agrobacterium rhizogenes oncogenic loci (rol)B rolC genes. The accumulation nitrogenous secondary metabolites significantly improved transgenic cell cultures. Specifically,...
This study delves into the novel utilization of Aristolochia manshuriensis cultured cells for extracellular silver nanoparticles (AgNPs) synthesis without need additional substances. The presence elemental has been verified using energy-dispersive X-ray spectroscopy, while distinct surface plasmon resonance peaks were revealed by UV-Vis spectra. Transmission and scanning electron microscopy indicated that AgNPs, ranging in size from 10 to 40 nm, exhibited a spherical morphology....
One of the hallmarks cancer is c hromosome in stability (CIN), which leads to aneuploidy, translocations, and other chromosome aberrations. However, vast majority human tumors molecular basis CIN remains unknown, partly because not all genes controlling transmission have yet been identified. To address this question, we developed an experimental high-throughput imaging (HTI) siRNA assay that allows identification novel genes. Our method uses a artificial (HAC) expressing GFP transgene. When...
Cell and tissue nanomechanics has been intriguingly introduced into biomedical research, not only complementing traditional immunophenotyping molecular analysis, but also bringing unexpected new insights for clinical diagnostics bioengineering. However, despite the progress in study of individual cells culture by atomic force microscopy (AFM), its application mapping live tissues a number technical limitations. Here, we elaborate technique to slices normal brain tumors combining...
Small peptides compose a large share of the mitochondrial proteome. Mitoregulin (Mtln) is peptide known to contribute respiratory complex I functioning and other processes in mitochondria. In our previous studies, we demonstrated that Mtln knockout mice develop obesity accumulate triglycerides oxidation substrates serum, concomitant with an exhaustion tricarboxylic acids cycle intermediates. Here examined functional role skeletal muscles, one major energy consuming tissues. We observed...
Prospective adjuvant anticancer therapy development includes the establishing of drug delivery systems based on biocompatible and biodegradable carriers. We have designed films nanoparticles (NPs) low-esterified pectin hydrogel using ionic gelation method. investigated morphology, nanomechanical properties, biocompatibility activity. Hydrogel are characterized by tunable viscoelastic properties surface nanoarchitectonics through concentration esterification degree (DE), expressed in variable...
Conventional chemotherapeutic approaches currently used for brain tumor treatment have low efficiency in targeted drug delivery and often non-target toxicity. Development of stable effective vehicles the most incurable diseases is one urgent biomedical challenges. We developed polymer nanoparticles (NPs) with improved temozolomide (TMZ) promising therapy, performing a rational design polyelectrolyte complexes oppositely charged polysaccharides cationic chitosan anionic pectin. The NPs’...