A5082452249- Cellular Mechanics and Interactions
- Glioma Diagnosis and Treatment
- Reproductive biology and impacts on aquatic species
- Aquaculture Nutrition and Growth
- Graphene and Nanomaterials Applications
- RNA Interference and Gene Delivery
- Tissue Engineering and Regenerative Medicine
- Force Microscopy Techniques and Applications
- Animal Genetics and Reproduction
- MicroRNA in disease regulation
- Advanced Electron Microscopy Techniques and Applications
- Telomeres, Telomerase, and Senescence
- Fish Ecology and Management Studies
- Yersinia bacterium, plague, ectoparasites research
- Protein Structure and Dynamics
- Heavy Metals in Plants
- Circular RNAs in diseases
- DNA Repair Mechanisms
- Marine Biology and Ecology Research
- Bone Tissue Engineering Materials
- Planarian Biology and Electrostimulation
- Nanoparticle-Based Drug Delivery
- Marine Sponges and Natural Products
- Microfluidic and Bio-sensing Technologies
- Fungal Plant Pathogen Control
Far Eastern Federal University
2015-2025
A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences
2011-2025
Far Eastern Branch of the Russian Academy of Sciences
2022
Russian Academy of Sciences
2011-2019
Abstract Glioma is a devastating brain tumor with high mortality rate attributed to the glioma stem cells (GSCs) possessing plasticity. Marker mutations in isocitrate dehydrogenase type 1 ( IDH1 ) and protein 53 TP53 are frequent gliomas impact cell fate decisions. Understanding GSC heterogeneity within IDH1- TP53- mutant tumors may elucidate possible treatment targets. Here, we performed single-nucleus transcriptomics of wild-type samples sorted for Sox2 marker. For first time rare...
Recently revised brain tumor classification suggested a glioma treatment strategy that takes into consideration molecular variants in IDH1 and TP53 marker genes. While pathogenic of can be accompanied by chromosomal instability (CIN), the impact mutations on genome stability remains unstudied. Elevated CIN might provide therapeutic targets, based synergistic effects chemotherapy with CIN-inducing drugs. Using an assay human artificial chromosomes, we investigated common missense chromosome...
Aristolochia manshuriensis is a relic liana, which widely used in traditional Chinese herbal medicine and endemic to the Manchurian floristic region. Since this plant rare slow-growing, alternative sources of its valuable compounds could be explored. Herein, we established hairy root cultures A. transformed with Agrobacterium rhizogenes oncogenic loci (rol)B rolC genes. The accumulation nitrogenous secondary metabolites significantly improved transgenic cell cultures. Specifically,...
Cell and tissue nanomechanics has been intriguingly introduced into biomedical research, not only complementing traditional immunophenotyping molecular analysis, but also bringing unexpected new insights for clinical diagnostics bioengineering. However, despite the progress in study of individual cells culture by atomic force microscopy (AFM), its application mapping live tissues a number technical limitations. Here, we elaborate technique to slices normal brain tumors combining...
The aim of this study is to trace how the fine-thermohaline and kinematic structure, formed over a section along 45.8° S in interaction zone outer Patagonian Shelf (PS) Malvinas (Falkland) Current (MC) System waters, affect spatial distribution bio-optical characteristics, phyto/zooplankton, birds, marine mammals. For first time, simultaneous multidisciplinary observations at high resolution (~2.5 km) were performed region during cruise R/V “Akademic Mstislav Keldysh” February 2022. A fine...
The effect of cultivation temperatures (37, 26, and 18 °C) on the conformational quality Yersinia pseudotuberculosis phospholipase A1 (PldA) in inclusion bodies (IBs) was studied using green fluorescent protein (GFP) as a folding reporter. GFP fused to C-terminus PldA form PldA-GFP chimeric protein. It found that maximum level fluorescence expression is observed cells grown at °C, while 37 °C no formation fluorescently active forms occurs. size, stability denaturant solutions, enzymatic...
C1q domain-containing (C1qDC) proteins are a group of biopolymers involved in immune response as pattern recognition receptors (PRRs) lectin-like manner. A new protein MkC1qDC from the hemolymph plasma
Mutations in IDH1 and TP53 have a significant impact on glioma prognosis progression; however, their roles tumor cell invasion terms of interactions with particular components the extracellular matrix (ECM) are still unclear. Using gene editing protocol based CRISPR-Cas 9 cytidine deaminase, we introduced point mutations into U87MG glioblastoma cells to establish modified lines heterozygous R132H, homozygous R248Q genotypes. A comparative study migration major ECM was carried out by...
Atomic force microscopy (AFM) recently burst into biomedicine, providing morphological and functional characteristics of cancer cells their microenvironment responsible for tumor invasion progression, although the novelty this assay needs to coordinate malignant profiles patients' specimens diagnostically valuable criteria. Applying high-resolution semi-contact AFM mapping on an extended number cells, we analyzed nanomechanical properties glioma early-passage cell cultures with a different...
Oncological diseases are a major focus in medicine, with millions diagnosed each year, leading researchers to seek new diagnostic and treatment methods. One promising avenue is the development of targeted therapies rapid tests using recognition molecules. The pharmaceutical industry increasingly exploring nucleic acid-based therapeutics. However, producing long oligonucleotides, especially aptamers, poses significant production challenges.
Human artificial chromosomes (HACs) have provided a useful tool to study kinetochore structure and function, gene delivery, expression. The HAC propagates segregates properly in the cells. Recently, we developed an experimental high-throughput imaging (HTI) HAC-based assay that allows identification of genes whose depletion leads chromosome instability (CIN). carries
The structure and properties of implanted materials based on modified pectins have been investigated, the proposed mechanism their action cells nervous tissue has shown.
Phenotyping and genotyping of tumor cells various genesis provides irreplaceable information for targeted therapy. We have revealed the nanomechanical properties living cultures primary cell lines gliomas with IDH1 R132H mutation, which make it possible to differentiate them from that do not this is necessary personalized therapy these diseases.
A novel C1qDC bivalve protein from the Modiolus kurilensis (MkC1qDC-1) was identified, isolated and characterized. MkC1qDC-1 demonstrated inhibition of HeLa cell proliferation recognition some cancer phenotypes in comparative analysis on several lines, suggesting biomedical potential this protein.
Method for nanomechanical and morphological mapping of the brain using atomic force microscopy (AFM) on live slices has been developed. The conditions parameters obtaining that meet requirements AFM a composite hydrogel have selected, necessary characteristics probes established. On slices, maps normal tissues glioblastoma were obtained.