A5029180364- Advanced biosensing and bioanalysis techniques
- Electrochemical sensors and biosensors
- Biosensors and Analytical Detection
- Analytical Chemistry and Sensors
- Nanopore and Nanochannel Transport Studies
- Graphene research and applications
- Electrochemical Analysis and Applications
- Molecular Junctions and Nanostructures
- Conducting polymers and applications
- MXene and MAX Phase Materials
- RNA Interference and Gene Delivery
- Advanced Biosensing Techniques and Applications
- Microfluidic and Capillary Electrophoresis Applications
- Lipid Membrane Structure and Behavior
- 2D Materials and Applications
- Pneumonia and Respiratory Infections
- Gene expression and cancer classification
- Advanced Chemical Sensor Technologies
- Microbial infections and disease research
- Perovskite Materials and Applications
- DNA and Nucleic Acid Chemistry
- Nanowire Synthesis and Applications
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- MicroRNA in disease regulation
Chongqing Institute of Green and Intelligent Technology
2016-2025
University of Chinese Academy of Sciences
2021-2024
Chinese Academy of Sciences
2017-2021
Southwest University
2020
Harbin Veterinary Research Institute
2020
Chinese Academy of Agricultural Sciences
2020
Chongqing University
2017
Institut Pasteur de Tunis
2016
University of California, Riverside
2010-2014
University of California System
2014
Here, we integrate two complementary detection strategies for the identification and quantification of Escherichia coli based on bacteriophage T4 as a natural bioreceptor living bacteria cells. The first approach involves screening viability assays, employing recognition element in label-free electrochemical impedance spectroscopy. is confirmation by loop-mediated isothermal amplification (LAMP) to amplify specifically E. Tuf gene after lysis bound cells, followed using linear sweep...
The objective of this study was to develop a biosensor using the cell wall binding domain (CBD) bacteriophage-encoded peptidoglycan hydrolases (endolysin) immobilized on gold screen printed electrode (SPE) and subsequent electrochemical impedance spectroscopy (EIS) for rapid specific detection Listeria cells. endolysin amine-coupled SPEs EDC/NHS chemistry. CBD-based used capture detect innocua serovar 6b from pure culture 2% artificially contaminated milk. In our study, functionalized have...
The efficiency of current microcystin detection methods has been hampered by the low limits required in drinking water and that routine is restricted to a few congeners with high degree undesired cross-reactivity. Here, we report development novel microcystin-targeting molecules their application detection. We have selected DNA aptamers from diverse random library exhibit affinity specificity microcystin-LR, -YR, -LA. obtained bind all chosen KD ranging 28 60 nM. More importantly, also are...
Transition-metal dichalcogenides (TMDs), including molybdenum disulfide (MoS2) and tungsten (WS2), with appealing properties have recently become promising alternatives to graphene semimetal low on/off current ratio as the sensing channel in field-effect transistor (FET) biosensors. However, efficiency of DNA-based FET devices strongly depends on how DNA probes are tethered nanomaterial channels. As against covalent attachment, simple physisorption has increasingly popular, a sequence strong...
Single-walled carbon nanotubes (SWCNTs) have attracted great attention on account of their superior and tunable electrical properties for promising applications in low-cost high-performance nano-electronics thin-film devices.
The poly(aniline boronic acid) (PABA)-functionalized single-walled carbon nanotube (SWNT) non-enzymatic sensor was developed for the detection of saccharides. work involved electrochemical polymerization 3-aminophenylboronic acid (3-APBA) in presence fluoride on surface SWNTs and their subsequent evaluation as chemiresistive sensors towards d-fructose d-glucose. By varying sensor's synthesis conditions by charge-controlled electropolymerization, sensing performance systematically optimized....
The present work is focused on the development, analytical characterization and evaluation of selective sensitive SWNT-chemiresistor immunosensor for label-free detection salivary α-amylase (SAA). SWNTs were aligned to bridge lithographically patterned gold microelectrodes using AC dielectrophoresis followed by functionalization with anti-SAA antibodies. nano-immunosensors exhibited excellent sensitivity over clinically relevant range (19 308 U ml−1) a limit (LOD) 6 µg ml−1 (0.6 7.8 (0.78 in...
Here we report single walled-carbon nanotubes (SWNTs)-based chemiresistor aptasensors for highly sensitive and selective detection of weakly or uncharged molecules using the displacement format. As a proof-of-concept demonstrate ATP, small charged molecule, by ssDNA anti-ATP aptamer hybridized to capture oligonucleotide covalently attached on SWNTs, with picomolar sensitivity selectivity over GTP.
A versatile and highly sensitive strategy for nanopore detection of microcystin-LR (MC-LR) is proposed herein based on the aptamer host–guest interactions by employing a gold nanoparticle (AuNP) probe. The MC-LR its complementary DNA (cDNA) are respectively immobilized AuNPs with distinct sizes (5 nm 20 cDNA), constructed polymeric AuNP network via hybridization cDNA was disintegrated upon addition MC-LR. specific between disrupt release cDNA-AuNPs that were then removed centrifugation,...
Researchers have recently designed various biosensors combining magnetic beads (MBs) and duplex-specific nuclease (DSN) enzyme to detect miRNAs. Yet, the interfacial mechanisms for surface-based hybridization DSN-assisted target recycling are relatively not well understood. Thus, herein, we developed a highly sensitive selective fluorescent biosensor study phenomenon that occurs on local microenvironment surrounding MB-tethered DNA probe via detecting microRNA-21 as model. Using above...
Modifying the electrical properties of fibroblasts against various glucose concentrations can serve as a basis for new, original sensing device. The aim present study is to test new biosensor based on impedancemetry measurement using eukaryote cells. Fibroblast cells were grown small optically transparent indium tin oxide semiconductor electrode. Electrochemical impedance spectroscopy (EIS) was used measure effect d-glucose fibroblast Further analyses EIS results performed equivalent...
To make ultrathin films for the fabrication of artificial olfactory systems, odorant biosensors, we have investigated mixed Langmuir and Langmuir−Blodgett odorant-binding protein/amphiphile. Under optimized experimental conditions (phosphate buffer solution, pH 7.5, OBP-1F concentration 4 mg L-1, target pressure 35 mN m-1), monolayer at air/water interface is very stable has been efficiently transferred onto gold supports, which were previously functionalized by self-assembled monolayers...
Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence analysis microarray spots hybridized QD labeled target indicated characteristic value 18.8 ns, compared to 13.3 ns obtained free solution, revealing that labels are sensitive spot microenvironment. Additionally, time gated was shown improve image contrast ratio by 1.8, achieving femtomolar sensitivity....
Monolayer WS₂ (Tungsten Disulfide) with a direct-energy gap and excellent photoluminescence quantum yield at room temperature shows potential applications in optoelectronics. However, controllable synthesis of large-area monolayer is still challenging because the difficulty controlling interrelated growth parameters. Herein, we report facile method for flakes by direct sulfurization powdered WO₃ Trioxide) drop-casted on SiO₂/Si substrates one-end sealed quartz tube. The samples were...