Abstract A variety of configurations and formats have been devised to exploit the phenomenon surface plasmon on metal dielectric interfaces for sensing a significant analytes, such as pesticides explosives, pathogens toxins, diseased tissue. Researchers continue aim at detecting lower concentrations in smaller volumes samples real time. new research field, called nanoplasmnonics, has emerged this regard.

Collection of nasopharyngeal samples using swabs followed by the transfer virus into a solution and an RNA extraction step to perform reverse transcription polymerase chain reaction (PCR) is primary method currently used for diagnosis COVID-19. However, need several reagents steps high cost PCR hinder its worldwide implementation contain outbreak. Here, we report cotton-tipped electrochemical immunosensor detection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen. Unlike...

A novel method is presented for the specific and direct detection of bacteria using bacteriophages as recognition receptors immobilized covalently onto functionalized screen-printed carbon electrode (SPE) microarrays. The SPE networks were through electrochemical oxidation in acidic media 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) by applying a potential +2.2 V to working electrode. Immobilization T4 bacteriophage SPEs was achieved via EDC formation amide bonds between protein...

Rising progesterone (P4) levels in humans due to its overconsumption through hormonal therapy, food products, or drinking water can lead many negative health effects. Thus, the simple and accurate assessment of P4 both environmental clinical samples is highly important protect public health. In this work, we present selection, identification, characterization ssDNA aptamers with high binding affinity P4. The were selected vitro from a single-stranded DNA library 1.8 × 10(15) oligonucleotides...

Rapid, sensitive, and selective pathogen detection is of paramount importance in infectious disease diagnosis treatment monitoring. Currently available diagnostic assays based on polymerase chain reaction (PCR) enzyme-linked immunosorbent assay (ELISA) are time-consuming, complex, relatively expensive, thus limiting their utility resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been used extensively the development rapid sensitive for nucleic acid...

We have demonstrated sensitive label-free virus detection using the opto-fluidic ring resonator (OFRR) sensor. The OFRR is a novel sensing platform that integrates microfluidics and photonic technology with low limit small volume. In our experiment, filamentous bacteriophage M13 was used as safe model system. Virus samples were flowed through whose surface coated M13-specific antibodies. studied sensor performance by monitoring in real-time antibody interaction. It shown can detect high...

This article reports a novel aptamer-based impedimetric detection of carbendazim, commonly used benzimidazole fungicide in agriculture. High affinity and specificity DNA aptamers against carbendazim were successfully selected using systematic evolution ligand by exponential enrichment (SELEX). The dissociation constants (Kds) the after 10 vitro selection cycles characterized fluorescence-based assays showing values nanomolar range. aptamer which showed highest degree conformation change was...

The development of successful biosensing platforms is highly dependent upon the biorecognition properties recognition receptor and sensitivity transducer binding signal. integration high affinity specificity DNA aptamers with unique carbon nanomaterial graphene offers an excellent avenue for sensitive selective architectures. In this work, a aptasensor which utilizes unlabeled aptamer assembled on electrode microcystin-LR detection was developed. A facile strategy used fabrication basis...

Here, we integrate two complementary detection strategies for the identification and quantification of Escherichia coli based on bacteriophage T4 as a natural bioreceptor living bacteria cells. The first approach involves screening viability assays, employing recognition element in label-free electrochemical impedance spectroscopy. is confirmation by loop-mediated isothermal amplification (LAMP) to amplify specifically E. Tuf gene after lysis bound cells, followed using linear sweep...

The objective of this study was to develop a biosensor using the cell wall binding domain (CBD) bacteriophage-encoded peptidoglycan hydrolases (endolysin) immobilized on gold screen printed electrode (SPE) and subsequent electrochemical impedance spectroscopy (EIS) for rapid specific detection Listeria cells. endolysin amine-coupled SPEs EDC/NHS chemistry. CBD-based used capture detect innocua serovar 6b from pure culture 2% artificially contaminated milk. In our study, functionalized have...

This work describes the selection and identification of DNA aptamers that bind with high affinity specificity to okadaic acid (OA), a lipophilic marine biotoxin accumulates in shellfish. The selected using systematic evolution ligands by exponential enrichment (SELEX) exhibited dissociation constants nanomolar range. aptamer highest was then used for fabrication label-free electrochemical biosensor detection. first immobilized on gold electrode self-assembly approach through Au-S...

The efficiency of current microcystin detection methods has been hampered by the low limits required in drinking water and that routine is restricted to a few congeners with high degree undesired cross-reactivity. Here, we report development novel microcystin-targeting molecules their application detection. We have selected DNA aptamers from diverse random library exhibit affinity specificity microcystin-LR, -YR, -LA. obtained bind all chosen KD ranging 28 60 nM. More importantly, also are...