A5054084616- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Viral Infections and Immunology Research
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Surfactants and Colloidal Systems
- Graphene and Nanomaterials Applications
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Lanthanide and Transition Metal Complexes
- Respiratory viral infections research
- Nanopore and Nanochannel Transport Studies
- biodegradable polymer synthesis and properties
- Animal Virus Infections Studies
- Magnetism in coordination complexes
- Carbon Nanotubes in Composites
- Influenza Virus Research Studies
- Nanoplatforms for cancer theranostics
- Advanced Polymer Synthesis and Characterization
- Advanced MRI Techniques and Applications
- Sphingolipid Metabolism and Signaling
- Invertebrate Immune Response Mechanisms
- RNA regulation and disease
- Statistical Methods in Clinical Trials
CSIRO Manufacturing
2018
Australian Centre for Disease Preparedness
2008-2017
CSIRO Health and Biosecurity
2016-2017
NIHR Bristol Cardiovascular Biomedical Research Unit
2017
Commonwealth Scientific and Industrial Research Organisation
2008-2016
Animal, Food and Health Sciences
2013-2014
Poultry CRC
2008-2013
University of Sussex
2004-2006
The University of Melbourne
2000-2002
Gaining an increased understanding of the toxicity new lipid nanoparticle formulations such as class cubic and hexagonal phase forming nanomaterials called cubosomes™ hexosomes™ is crucial for their development therapeutic agents. Surprisingly, literature on in vitro vivo nanoparticles negligible, despite a rapidly growing number publications potential use various applications. In this work we have developed methods to study cytotoxicity two chemically distinct dispersions using lipids...
Monoolein–capric acid combinations form into particles with internal nanostructures, including inverse hexagonal and bicontinuous cubic mesophases, differing cytotoxicity.
Lyotropic liquid crystalline nanoparticle dispersions are of interest as delivery vectors for biomedicine. Aqueous liposomes, cubosomes, and hexosomes commonly stabilized by nonionic amphiphilic block copolymers to prevent flocculation phase separation. Pluronic stabilizers such F127 used; however, there is increasing in using chemically reactive enhanced functionalization specificity therapeutic applications. This study has explored the ability...
We present studies of the delivery short interfering ribonucleic acid (siRNA) into a green fluorescent protein (GFP) expressing cell line, using lipid nanocarriers in cubic lyotropic liquid crystal form. These carriers are based on glycerol monooleate (GMO) and employ use varying concentrations cationic siRNA binding lipids. The essential physicochemical parameters lipid/GMO/siRNA complexes such as particle size, ζ otential, uptake stability, mesophase behavior, cytotoxicity,and gene...
The purpose of this work was to synthesize and screen, for their effectiveness act as T1-enhancing magnetic resonance imaging (MRI) contrast agents, a small library nitroxide lipids incorporated into cubic-phase lipid nanoparticles (cubosomes). most effective then formulated lower-toxicity (hexosomes), MR observed in the aorta spleen live rats vivo. This new class allowed higher relaxivities on order those clinically used gadolinium complexes. hexosome formulation presented herein...
Self-assembled lipid lyotropic liquid crystalline nanoparticles such as hexosomes and cubosomes contain internal anisotropic isotropic nanostructures, respectively. Despite the remarkable potential of in various biomedical applications, stabilisers used formulating are often limited to commercially available polymers Pluronic block copolymers. This study explored using Reversible Addition-Fragmentation chain Transfer (RAFT) technology design amphiphilic brush-type for purpose stabilising...
Eukaryotic initiation factor (eIF) 4G is an integral member of the translation machinery. The molecule serves as a scaffold for several other factors, including eIF4E, eIF4AI, eIF3 complex, and poly(A)-binding protein (PABP). Previous work indicates that complexes between these proteins exhibit enhanced mRNA cap-binding RNA helicase activities relative to respective individual proteins, eIF4E eIF4A. eIF4G-PABP interaction has been implicated in enhancing formation 48 S 80 ribosome recycling...
This study reports on the synthesis and characterization of a novel four-arm reagent its use in core degradable star polymers block copolymers using reversible addition–fragmentation chain transfer (RAFT) polymerization process. The RAFT prepared from methyl methacrylate (MMA), styrene (ST) N,N-dimethylacrylamide (DMA) were characterized by size exclusion chromatography (SEC). PMMA polymer was further polymerized with poly(ethylene glycol) ether (POEGMA8–9) to produce copolymer....
Crustaceans form their distinct patterns and colors through the interaction of carotenoid astaxanthin with a protein called crustacyanin (CRCN). Presently, expression just two CRCN genes is thought to provide subunits that combine complex associated colour change from red blue. This study aimed explore genetic complexity underlying production pigmentation camouflage in penaeid shrimp. We isolated 35 new 12 species, sequence analysis indicated this gene family has undergone significant...
Biophysical studies were undertaken to investigate the binding and release of short interfering ribonucleic acid (siRNA) from lyotropic liquid crystalline lipid nanoparticles (LNPs) by using a quartz crystal microbalance (QCM). These carriers are based on phytantriol (Phy) cationic DOTAP (1,2-dioleoyloxy-3-(trimethylammonium)propane). The nonlamellar phase LNPs tethered surface QCM chip for analysis biotin-neutravidin binding, which enabled controlled deposition siRNA-LNP complexes with...
The foot-and-mouth disease virus (FMDV) leader (L) proteinase is an important virulence determinant in FMDV infections. It possesses two distinct catalytic activities: (i) C-terminal processing at the L/VP4 junction; and (ii) induction of cleavage translation initiation factor eIF4G, event that inhibits cap-dependent infected cells. only other member Aphthovirus genus, equine rhinitis A (ERAV), also encodes L protein, but this shares 32% amino acid identity with its counterpart. Another more...
The use of medical imaging contrast agents may lead to improved patient prognosis by potentially enabling an earlier detection diseases and therefore initiation treatments. In this study, we fabricated superparamagnetic iron oxide (SPIO) nanoparticles within the inner cavity multiwalled carbon nanotubes (MWCNTs) for first time; thereby ensuring high mechanical stability nanoparticles. A simple, but effective, self-assembled coating with RAFT diblock copolymers ensured SPIO-MWCNTs have a...
The eukaryotic initiation factor eIF4GI plays a central role in the assembly of competent complex at 5′ end an mRNA. Five isoforms eIF4G exist cells, arising from alternative translation initiation. During picornaviral infection or apoptosis, is cleaved proteolytically to yield distinct fragments. Using HeLa we have examined fate these proteins cell. We found that while endogenous predominantly cytoplasmic, population can also be visualised nucleus. Furthermore, localised primarily nuclear...
Animal agriculture faces a broad array of challenges, ranging from disease threats to adverse environmental conditions, while attempting increase productivity using fewer resources. RNA interference (RNAi) is biological phenomenon with the potential provide novel solutions some these challenges. Discovered just 20 years ago, mechanisms underlying RNAi are now well described in plants and animals. Intracellular double-stranded triggers conserved response that leads cleavage degradation...
The translation of siRNA into clinical therapies has been significantly delayed by issues surrounding the delivery naked to target cells. Here we investigate cationic acrylic polymers developed Reversible Addition-Fragmentation chain Transfer (RAFT) mediated free radical polymerization. We investigated cell uptake and gene silencing a series siRNA-star polymer complexes both in presence absence protein "corona". Using multidisciplinary approach including quantitative nanoscale...
The requirement for new antiviral therapeutics is an ever present need. Particularly lacking are broad spectrum antivirals that have low toxicity. We develop such agents based on macromolecular prodrugs whereby both the polymer chain and drug released from upon cell entry effects. Specifically, were designed herein poly(methacrylic acid) ribavirin. Structure–function parameter space was analyzed via synthesis of 10 compositions varied by molar mass content. Antiviral activity tested in...
ABSTRACT Equine rhinitis A virus (ERAV) has recently been classified as an aphthovirus, a genus otherwise comprised of the different serotypes Foot-and-mouth disease (FMDV). FMDV initiates translation via type II internal ribosomal entry site (IRES) and utilizes two in-frame AUG codons to produce leader proteinases Lab Lb. Here we show that ERAV 5′ nontranslated region also possesses core structures IRES. The functional activity this was characterized by transfection bicistronic plasmids...