A5077661269- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Renal and related cancers
- Monoclonal and Polyclonal Antibodies Research
- Congenital heart defects research
- Healthcare and Environmental Waste Management
- Tissue Engineering and Regenerative Medicine
- Advanced Nanomaterials in Catalysis
- Mathematical Biology Tumor Growth
- Pluripotent Stem Cells Research
- Nanoparticles: synthesis and applications
- Trace Elements in Health
- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Anesthesia and Neurotoxicity Research
- interferon and immune responses
- Liver Disease and Transplantation
Gladstone Institutes
2020-2023
University of Nebraska–Lincoln
2015-2021
Liver fibrosis occurs as a consequence of chronic injuries from viral infections, metabolic disorders, and alcohol abuse. Fibrotic liver microenvironment (LME) is characterized by excessive deposition aberrant turnover extracellular matrix proteins, which leads to increased tissue stiffness. stiffness acts vital cue in the regulation hepatic responses both healthy diseased states; however, effect varying on cells not well understood. There critical need engineer vitro models that mimic...
Titanium dioxide (TiO2) nanoparticles are one of the most highly manufactured and employed nanomaterials in world with applications copious industrial consumer products. The liver is a major accumulation site for many nanoparticles, including TiO2, directly through intentional exposure or indirectly unintentional ingestion via water, food animals increased environmental contamination. Growing concerns over current usage TiO2 coupled lack mechanistic understanding its potential health risk...
TiO<sub>2</sub>nanoparticle exposure to primary astrocytes induced concentration dependent loss in glutamate uptake, morphological changes mitochondria (tabulation or fragmentation) and damage mitochondrial dynamics.
Hepatitis C virus (HCV) remains a global public health challenge with an estimated 71 million people chronically infected, surges in new cases and no effective vaccine. New methods are needed to study the human immune response HCV since vivo animal models limited vitro cancer cell often show dysregulated proliferative responses. Here, we developed CD8 + T adult stem liver organoid system using microfluidic chip coculture 3D organoids embedded extracellular matrix HLA-matched primary cells...
Abstract During embryogenesis, paracrine signaling between tissues in close proximity contributes to the determination of their respective cell fate(s) and development into functional organs. Organoids are vitro models that mimic organ formation cellular heterogeneity, but lack input surrounding tissues. Here, we describe a human multilineage iPSC-derived organoid recapitulates cooperative cardiac gut displays extensive structural complexity both We demonstrate presence endoderm tissue...
The hepatitis C virus (HCV) causes liver disease, affecting millions. Even though we have effective antivirals that cure HCV, they cannot stop terminal disease. We used an adult stem cell-derived organoid system to understand how HCV infection leads the progression of Here, show maintains low-grade infections in organoids for first time. transcriptional reprogramming causing cancer cell development and altered immune response. Our finding shows mimics patient pathogenesis. These results...
Chronic liver disease is characterized by progressive hepatic fibrosis leading to the formation of cirrhosis irrespective etiology with no effective treatment currently available. Liver stiffness (LS) best clinical predictor this progression etiology. LS and hepatocytes-nonparenchymal cells (NPC) interactions are two variables known be important in regulating function during fibrosis, but little about interplay these cues. Here, we use polydimethyl siloxane (PDMS) based substrates tunable...
Abstract Liver sinusoidal endothelial cells (LSECs) are a highly specialized cell that participates in numerous liver metabolic activities and collectively function as scavenger system the by removing waste macromolecules playing vital role balance of lipids, cholesterol, vitamins. Prior to hepatic fibrosis, independent their etiology, LSECs become pro-inflammatory, capillarized (loss fenestrations), loss receptors (Stabilin-1, Stabilin-2, CD31 SE-1). fibrosis also leads significant...
Background Liver sinusoidal endothelial cells (LSECs) are a highly specialized cell that participates in numerous liver metabolic activities and collectively function as scavenger system the by removing waste macromolecules playing vital role balance of lipids, cholesterol, vitamins. Prior to hepatic fibrosis, independent their etiology, LSECs become pro‐inflammatory, capillarized (loss fenestrations), loss receptors (Stabilin‐1, Stabilin‐2, CD31 SE‐1). fibrosis also leads significant...
Abstract Hepatitis C virus (HCV) remains a global public health challenge with an estimated 71 million people chronically infected, surges in new cases and no effective vaccine. New methods are needed to study the human immune response HCV since vivo animal models limited vitro cancer cell often show dysregulated proliferative responses. Here we developed CD8 + T adult stem liver organoid system using microfluidic chip coculture 3D organoids embedded extracellular matrix HLA-matched primary...
Summary Hepatitis C virus (HCV) is the leading cause of death from liver disease. How HCV infection causes lasting damage and increases cancer risk beyond viral clearance remains unclear. We identify bipotent stem cells as novel targets for infection, their erroneous differentiation potential impaired regeneration development. show 3D organoids generated actively HCV-infected individuals carry replicating maintain low-grade over months. Organoids can be infected with a primary isolate....