Ferroptosis is a novel form of iron-dependent cell death and participates in the malignant progression glioblastoma (GBM). Although circular RNAs (circRNAs) are found to play key roles ferroptosis via several mechanisms, including regulating iron metabolism, glutathione lipid peroxidation mitochondrial-related proteins, there many circRNAs need be found, they may become new molecular treatment target GBM.The expression levels circLRFN5, PRRX2 GCH1 were detected by qPCR, western blotting,...

Gliomas are the most malignant and aggressive primary brain tumor in adults. Despite concerted efforts to improve therapies, their prognosis remains very poor. Isocitrate dehydrogenase 1 (IDH1) mutations have been discovered frequently glioma patients strongly correlated with improved survival. However, effect of IDH1 on chemosensitivity gliomas unclear. In this study, we generated clonal U87 U251 cell lines overexpressing R132H mutant protein (IDH1-R132H). Compared control cells IDH wild...

Glioma exhibit heterogeneous susceptibility for targeted ferroptosis. How circRNAs alterations in glioma promote iron metabolism and ferroptosis defense remains unclarified.The highly enriched glioblastoma (GBM) were obtained through analysis of sequencing datasets. Quantitative real-time PCR (qRT-PCR) was used to determine the expression circRNF10 normal brain tissue. Both gain-of-function loss-of-function studies assess effects on using vitro vivo assays. The hypothesis that ZBTB48...

Chemoresistance of glioblastoma (GBM) is a feature this devastating disease. This study to determine the relationship between Hedgehog (HH)/Gli1 signaling pathway and chemoresistance temozolomide (TMZ) in human GBM.We analyzed Gli1 nuclear staining O6-methylguanine DNA methyltransferase (MGMT) expression 48 cases primary GBM tissues by immunohistochemistry. Quantitative PCR, western blot, methylation-specific cell proliferation apoptosis assay were used investigate changes MGMT...

Glioma development is an extremely complex process with changes occurring in numerous genes. HOXD antisense growth-associated long noncoding RNA (HOXD-AS1), important (lncRNA), known to regulate metastasis-related gene expression bladder cancer, ovarian cancer and neuroblastoma. Here, we elucidated the function possible molecular mechanisms of lncRNA HOXD-AS1 human glioma cells. Our results proved that was upregulated tissues cell lines. overexpression promoted migration invasion vitro,...

Glioblastoma (GBM) is characterized by abundant neovascularization as an essential hallmark. Vasculogenic mimicry (VM) a predominant pattern of GBM neovascularization. However, the biological functions circRNAs prompting VM formation in remains unclarified.

Recent studies have shown that aberrant activation of Sonic Hedgehog (SHH)/GLI1 signaling pathway is involved in many kinds human malignancies. Both vitro and vivo experiments demonstrate inhibiting the results tumor regression for a fraction gliomas which harbor an active pathway. However, little known about linkage between SHH/GLI1 cell migration invasion gliomas. In this study, we showed GLI1 expression closely correlates with pathological grades by immunohistochemistry analysis 110 cases...

Traumatic brain injury (TBI) is induced by complex primary and secondary mechanisms that give rise to cell death, inflammation, neurological dysfunction. Understanding the drive damage as well those promote repair can guide development of therapeutic drugs for TBI. Kruppel-like factor 4 (KLF4) has been reported negatively regulate axon regeneration injured retinal ganglion cells (RGCs) through inhibition JAK-STAT3 signaling. However, role KLF4 in TBI remains unreported. Reactive oxygen...

Abstract Glioma stem cells (GSCs) are a special kind of in GBM showing tumor initiation, self-renewal, and multi-lineage differentiation abilities. Finding novel circRNAs related to GSCs is great significance for the study glioma. qPCR, western blotting, immunohistochemistry were used detect expression levels circKPNB1, SPI1, DGCR8, TNF-α. The these molecules was regulated by lentiviral-based infection. RNA immunoprecipitation assay, pull-down, dual-luciferase reporter, chromatin assays...

The objective of this study was to retrospectively the outcomes large vestibular schwannoma resection through suboccipital retrosigmoid keyhole approach and emphasize technical details advantages surgical schwannomas via approach.From January 2010 September 2012, 37 consecutive patients (16 men 21 women) with schwannoma, 4 cm or greater, received in our department. Clinical records, radiographic findings, operative summaries, follow-up data were analyzed retrospectively.The mean age these...

Glioblastoma (GBM), characterized by high morbidity and mortality, is one of the most common lethal diseases worldwide. To identify molecular mechanisms that contribute to development GBM, three cohort profile datasets (GSE50161, GSE90598 GSE104291) were integrated thoroughly analyzed; these included 57 GBM cases 22 normal brain tissue. The current study identified differentially expressed genes (DEGs), analyzed potential candidate pathways. Additionally, a DEGs‑associated protein‑protein...

Glioblastoma (GBM) is the most common primary malignant tumor in brain, and its robust proliferation invasion abilities reduce survival time of patients. Circular RNAs (circRNAs) play an essential role various tumors, such as regulating cell proliferation, apoptosis, invasion, metastasis, other progressive phenotypes through different mechanisms. Finding novel circRNAs may significantly contribute to prognosis GBM provide basis for targeted therapy GBM. In this study, we found circPTPRF a...

CACNA2D3, an auxiliary member of the alpha-2/delta subunit three family voltage-dependent calcium channel complex, plays a critical role in tumor suppression. However, its glioma carcinogenesis remains largely unknown. Here, we investigated putative suppressive CACNA2D3 gliomas. Downregulation was frequently detected tissues and cells compared with their non-tumorigenic counterparts, correlated poor survival. To investigate underlying mechanism development progression glioma, performed...

// Daming Cui 1, * , Pandey Sajan Jinlong Shi 2 Yiwen Shen 3 Ke Wang 1 Xianyu Deng Lin Zhou Pingping Hu Liang Gao Department of Neurosurgery, Shanghai Tenth People’s Hospital, Tongji University School Medicine, 200072, Republic China Affiliated Hospital Nantong University, 226001, Jiangsu Province, Huashan Fudan 200070, These two authors contributed equally to this paper Correspondence to: Gao, email: kalantes@163.com Keywords: GADD45A, miR-148a, β-catenin, migration, invasion...

The delivery of anticancer drugs to the brain is profoundly limited by blood-brain barrier (BBB)The purpose this work develop a new carrier for doxorubicin overcome BBB. Polyamidoamine (PAMAM) dendrimer, novel nanoscopic high-branching polymer, was exploited as an efficient doxorubicin. MTT assay showed that PAMAM (generation 3) had little cytotoxicity in capillary endothelial cells (BCECs). results fluorescence intensity and fluorescent microscopy cellular uptake of  PAMAM/doxorubicin...

Background: Hedgehog/Gli1 (HH/Gli1) pathway plays an important role in the patterning and development of central nervous system during embryogenesis. Recent data have shown its potential involvement a subset human gliomas inhibition resulted tumor suppression both vitro vivo studies. The underlying mechanisms suppression, however, remain to be fully elucidated. Materials Methods: Gli1 expression was investigated 60 surgically resected glioma tissues (World Health Organization (WHO) III-IV)....