A5007132946- MicroRNA in disease regulation
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Nuclear Structure and Function
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- Renal and related cancers
- Protein Degradation and Inhibitors
- Renal cell carcinoma treatment
- Circular RNAs in diseases
- Genetic and Kidney Cyst Diseases
- interferon and immune responses
- Cancer-related gene regulation
- Chronic Myeloid Leukemia Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Melanoma and MAPK Pathways
- Epigenetics and DNA Methylation
- Psoriasis: Treatment and Pathogenesis
- Dermatology and Skin Diseases
- RNA regulation and disease
- Kruppel-like factors research
Martin Luther University Halle-Wittenberg
2020-2024
University Hospital in Halle
2017-2019
Wittenberg University
2019
University Hospital Leipzig
2011-2018
Leipzig University
2013-2018
Tumor-associated macrophages (TAMs) are the most abundant immune cells in tumor microenvironment, promoting initiation, growth, progression, metastasis, and evasion. Recently it was shown that cancer cell-derived exosomes induce a tumor-promoting phenotype TAMs. Exosome-loaded proteins, DNA, RNAs may contribute to macrophage reprogramming. However, exact mediators mechanisms, particularly melanoma, not known. In this study we examined effects of cutaneous melanoma-derived on function...
Abstract Non-small cell lung cancer (NSCLC) is the leading cause of death worldwide underlining urgent need for new biomarkers and therapeutic targets this disease. Long noncoding RNAs are critical players in NSCLC but role small RNA species not well understood. In present study, we investigated H/ACA box nucleolar (snoRNAs) snoRNA-bound ribonucleoproteins (snoRNPs) tumorigenesis NSCLC. snoRNPs including NOP10 core protein were highly expressed High levels either mRNA or associated with poor...
In acute promyelocytic leukemia (APL), all-trans retinoic acid (ATRA) treatment induces granulocytic maturation and complete remission of leukemia. microRNAs are known to be critical players in the formation leukemic phenotype. this study, we report downregulation miR-181a/b gene cluster APL blasts NB4 cells upon ATRA as a key event drug response. We found that expression was activated by PML/RARα oncogene transgenic knock-in mice, an observation confirmed extended evidence enhanced patient...
Transcription factor C/EBPα is a master regulator of myelopoiesis and its inactivation associated with acute myeloid leukemia. Deregulation by microRNAs during granulopoiesis or leukemia development has not been studied. Here we show that oncogenic miR-182 strong C/EBPα. Moreover, identify regulatory loop between miR-182. While blocks expression direct promoter binding differentiation, enforced reduces protein level impairs in vitro vivo. In addition, highly elevated particularly patients...
Abstract In IgA vasculitis (IgAV) perivascular deposition of IgA1 immune complexes (IgA-ICs) is traditionally considered the fundamental trigger for polymorphonuclear neutrophil (PMN)–mediated damage. We propose that IgA-IC deposition, although mandatory, not sufficient alone IgAV. Serum levels and binding to PMNs were quantified in IgAV patients controls. Activation was evaluated by extracellular trap (NET) release, adherence, cytotoxicity assays a flow system mirror conditions at...
Abstract Hematopoiesis, the formation of blood cells from hematopoietic stem (HSC), is a highly regulated process. Since discovery microRNAs (miRNAs), several studies have shown their significant role in regulation system. Impaired expression miRNAs leads to disrupted cellular pathways and particular causes loss ability. Here, we report previously unrecognized function miR-143 granulopoiesis. Hematopoietic undergoing granulocytic differentiation exhibited increased expression. Overexpression...
Abstract In melanoma, carcinoma‐associated fibroblasts (CAFs) are important cellular components in the tumour microenvironment due to their potential promote growth and metastatic spread of malignant cells. Melanoma cells have ability affect non‐tumour by releasing extracellular vesicles (EVs). The mechanisms responsible for reprogramming normal dermal (NHDFs) into CAFs remain incompletely understood. However, it is likely thought be mediated melanoma‐specific miRNAs, which transported EVs...
Many melanomas are associated with activating BRAF mutation. Targeted therapies by inhibitors of and MEK (BRAFi, MEKi) show marked antitumor response, but become limited drug resistance. The mechanisms for this not fully revealed, include miRNA. Wishing to improve efficacy BRAFi knowing that certain miRNAs linked resistance BRAFi, we wanted focus on exclusively response BRAFi. We found increased expression miR-129-5p during treatment BRAF- mutant melanoma cells. Parallel emergence observed...
Abstract Background Renal cell carcinomas (RCC) are characterized by the deregulation of several hundred hyperosmolality-responsive genes. High expression a subset these genes including Ran binding protein 3 like ( RANBP3L ) is linked to favorable prognostic outcome in RCC. However, cellular function remains largely unknown. Methods We used CRISPR/Cas9-mediated gene editing generate functional deletions Ranbp3l and nuclear factor activated T cells 5 Nfat5 loci murine renal line. The NFAT5-KO...
Extracellular vesicles (EVs) are important mediators in the intercellular communication, influencing function and phenotype of different cell types within tumor micro-milieu thus promote progression. Since EVs safely transport packages proteins, lipids also nucleic acids such as miRNAs, their cargo can serve diagnostic prognostic markers. Therefore, aim this study was to investigate EV embedded miRNAs specific for melanoma, which could potential biomarkers. In contrast previous studies, we...
Active BCR related (ABR) gene deactivates ras-related C3 botulinum toxin substrate 1 (RAC1), which plays an essential role in regulating normal hematopoiesis and leukemia. gene, closely to ABR, acts as a tumor suppressor chronic myeloid leukemia has overlapping functions with ABR. Evidence for putative of ABR been shown several solid tumors, deletion is present. Our results show downregulation AML. A block prevents differentiation leads repression the transcription factor C/EBPα, major...
Activating BRAF mutations occurs in 50-60% of malignant melanomas. Although initially treatable, the development resistance to BRAF-targeted therapies (BRAFi) is a major challenge and limits their efficacy. We have previously shown that BRAFV600E signaling pathway mediates expression EZH2, an epigenetic regulator related melanoma progression worse overall survival. Therefore, we wondered whether inhibition EZH2 would be way overcome vemurafenib. found addition inhibitor vemurafenib improved...
Metastatic primary cutaneous melanoma is a frequently fatal disease despite recent therapeutic advances. Biomarkers to stratify patients’ prognosis are lacking. MicroRNAs (miRNAs) small, non-coding RNAs. We aimed determine the expression of miR-211-5p in tumors and metastases malignant its potential use as prognostic biomarker. performed situ hybridization for miRNA-211-5p on 109 FFPE samples from 76 patients, including 31 paired tumor/metastasis samples. For validation, we silico analyses...
Despite the constant development of innovative therapeutic options for hematological malignancies, gold-standard therapy regimen curative treatment often includes allogeneic hematopoietic stem cell transplantation (HSCT). The graft-versus-leukemia effect (GVL) is one main goals that arises from HSCT. On other hand, graft-versus-host disease (GVHD) still and most serious complications following In acute myeloid leukemia (AML), HSCT together with high-dose chemotherapy used as a option. An...
Invasive growth is a critical process in tumor progression, requiring the activation of various molecular processes cells at invasive front. Intercellular communication between heterogeneous enhances cellular and adaptation to specific microenvironments. One mechanism intercellular delivery miRNAs through cell-derived extracellular vesicles (EVs). In this context we have observed that conditioned media from highly cell subpopulation (BLM-HI) capacity parental line (BLM). Therefore,...