A5080682155- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neurogenesis and neuroplasticity mechanisms
- Nicotinic Acetylcholine Receptors Study
- Receptor Mechanisms and Signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Photoreceptor and optogenetics research
- Neuroendocrine regulation and behavior
- Lipid Membrane Structure and Behavior
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- GABA and Rice Research
- Autism Spectrum Disorder Research
- Cholinesterase and Neurodegenerative Diseases
- Neuroscience of respiration and sleep
- Neurotransmitter Receptor Influence on Behavior
- Regulation of Appetite and Obesity
- Stress Responses and Cortisol
- Nerve injury and regeneration
- Neonatal and fetal brain pathology
- Planarian Biology and Electrostimulation
Universidad Nacional Autónoma de México
2016-2025
Autonomous University of Queretaro
2001-2022
Colciencias
2010
Istituto Pasteur
2002-2005
University of California, Irvine
1997-2002
The identification of pathways necessary for retinal pigment epithelium (RPE) function is fundamental to uncover therapies blindness. Prolactin (PRL) receptors are expressed in the retina, but nothing known about role PRL RPE. Using adult RPE 19 (ARPE-19) human cell line and mouse RPE, we identified presence demonstrated that survival via anti-apoptotic antioxidant actions. promotes capacity ARPE-19 cells by reducing glutathione. It also blocks hydrogen peroxide-induced increase deacetylase...
The Xenopus oocyte is a very powerful tool for studies of the structure and function membrane proteins, e.g., messenger RNA extracted from brain injected into oocytes leads to synthesis incorporation many types functional receptors ion channels, vesicles Torpedo electroplaques fuse with cause appearance acetylcholine Cl − channels. This approach was developed further transplant already assembled neurotransmitter human cells plasma oocytes. Membranes isolated temporal neocortex patient,...
Abstract Mefenamic acid (MFA) has anti‐convulsant and pro‐convulsant effects in vivo, been shown to potentiate inhibit GABA A (γ‐aminobutyric acid) receptors vitro. In this study, whole‐cell currents were recorded from Xenopus oocytes human embryonic kidney (HEK) cells expressing recombinant resolve the molecular mechanisms by which MFA modulates receptor function. We demonstrate that potentiated GABA‐activated for α1β2 γ2S (EC 50 = 3.2 ± 0.5 μ m ), but not α1β1 receptors. also enhanced...
ABSTRACT Background Excitotoxicity is a process in which NADPH oxidase‐2 (NOX‐2) plays pivotal role the generation of reactive oxygen species (ROS). Oxidative stress influences expression Aquaporin 4 (AQP4), water channel implicated blood–brain barrier (BBB) permeability and edema formation. The endocannabinoid system widely distributed brain, particularly through cannabinoid receptor type 1 (CB1) 2 (CB2), have been shown to neuroprotective function brain injury. Given significant...
Intracerebral hemorrhage (ICH) is a leading cause of death and disability worldwide. Following the initial mechanical injury caused by hematoma expansion, secondary occurs, characterized production reactive oxygen species (ROS) generated NOX-2 neuroinflammation, which exacerbated upregulation NLRP3 inflammasome. These conditions collectively aggravate brain damage. The endocannabinoid system (ECS), through activation cannabinoid receptors, has demonstrated neuroprotective properties in...
The properties of γ-aminobutyric acid (GABA) type A receptors (GABA receptors) microtransplanted from the human epileptic brain to plasma membrane Xenopus oocytes were compared with those recorded directly neurons, or glial cells, in brains slices. Cell membranes isolated specimens, surgically obtained six patients afflicted drug-resistant temporal lobe epilepsy (TLE) injected into frog oocytes. Within a few hours, these acquired GABA that generated currents an unusual run-down, which was...
About a decade ago, cell membranes from the electric organ of Torpedo and rat brain were transplanted to frog oocytes, which thus acquired functional neurotransmitter receptors. Nevertheless, great potential that this method has for studying human diseases remained virtually untapped. Here, we show postmortem brains humans suffered Alzheimer's disease can be microtransplanted plasma membrane Xenopus oocytes. We also these carry receptors voltage-operated channels are still functional, even...
We studied the properties of GABAA receptors microtransplanted from human temporal lobe epilepsy (TLE)-associated brain regions to Xenopus oocytes. Cell membranes, isolated surgically resected specimens drug-resistant TLE patients, were injected into frog oocytes, which rapidly incorporated receptors, and any associated proteins, their surface membrane. The originating different epileptic had a similar run-down but an affinity for GABA that was approximately 60% lower subiculum than issuing...
The present review discusses the functional and molecular diversity of GABAρ receptors. These receptors were originally described in mammalian retina, their role visual pathway has been recently elucidated; however new studies on distribution brain spinal cord have revealed that they are more spread than thought, thus it will be important to determine physiological contribution GABAergic transmission other areas central nervous system. In addition, modeling peculiar traits these impacted...
Anorexia nervosa is an eating disorder associated with severe weight loss as a consequence of voluntary food intake avoidance. Animal models such dehydration-induced anorexia (DIA) mimic core features the disorder, including reduction in intake, which compromises supply energy to brain. Glial cells, major population nerve cells central nervous system, play crucial role supplying neurons. The corpus callosum (CC) largest white matter tract mammals, and more than 99% cell somata correspond...
Anorexia nervosa is an eating disorder observed primarily in young women. The neurobiology of the unknown but recently magnetic resonance imaging showed a volume reduction hippocampus anorexic patients. Dehydration-induced anorexia (DIA) murine model that mimics core features this disorder, including severe weight loss due to voluntary food intake. energy supply brain mediated by astrocytes, whether their density compromised unknown. Thus, aim study was estimate GFAP+ cell main regions (CA1,...
Neuronal nicotinic acetylcholine (AcCho) receptors composed of alpha7-subunits (alpha7-AcChoRs) are involved in many physiological activities. Nevertheless, very little is known about their single-channel characteristics. By using outside-out patch-clamp recordings from Xenopus oocytes expressing wild-type (wt) alpha7-AcChoRs, we identified two classes channel conductance: a low conductance (gamma(L)) 72 pS and high one (gamma(H)) 87 pS, with mean open-times (tau(op)) 0.6 ms. The same...
J. Neurochem. (2012) 122 , 900–910. Abstract GABAergic transmission in the neostriatum plays a central role motor coordination, which plethora of GABA‐A receptor subunits combine to modulate neural inhibition. GABAρ receptors were originally described mammalian retina. These possess special electrophysiological and pharmacological properties, forming characteristic class ionotropic receptors. In previous studies, we suggested that are expressed neostriatum, this report show they indeed...
The role of thyroid hormones (THs) in development has been extensively studied, however, the specific molecular mechanisms involved are far from being clear. THs act by binding to TH nuclear receptors (TR) that as ligand-dependent transcription factors regulate TH-dependent gene expression. Like vertebrates, zebrafish express different isoforms functional Tr alpha and beta, some which can bind alternative ligands like 3,5-T2. In this study, we first analyzed effects exogenous T3 3,5-T2...
The ρ1 γ-aminobutyrate receptor (GABA ) is expressed predominantly in the retina and forms homomeric GABA-gated Cl − channels that are clearly different from multisubunit GABA A receptors. In contrast to these, receptors desensitize very little not blocked by bicuculline. addition , two new variants were identified human cDNA libraries. Cloning sequence analysis showed both contain large deletions putative extracellular domain of receptor. These extend a common 5′ site 3′ sites. with largest...
Several chemical signals synthesized in the ovary, including neurotransmitters, have been proposed to serve as regulators of folliculogenesis, however, their mechanisms action not completely elucidated. Here, electrophysiological and molecular biology techniques were used study responses generated via purinergic stimulation cultured mouse cumulus cell-enclosed oocytes (CEOs). Application extracellular ATP elicited depolarizing CEOs. Using voltage clamp technique by impaling with two...
It is known that serotonin exerts its different nociceptive and motor functions by interacting with distinct receptors subtypes, which could be either G-protein coupled or ionotropic. Previous reports demonstrated the early activation of receptor transcripts during rat development, suggesting a potential role serotoninergic system ontogeny. In this study we have compared cellular distribution three subtypes: 5-HT2C, 5-HT5A, 5-HT7. Immunocytochemical methods were used in slices hippocampus...