A5009027988- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
- Hemoglobinopathies and Related Disorders
- Blood Coagulation and Thrombosis Mechanisms
- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Cell death mechanisms and regulation
- Blood properties and coagulation
- Acute Ischemic Stroke Management
- Cell Adhesion Molecules Research
- Hemophilia Treatment and Research
- Atherosclerosis and Cardiovascular Diseases
- Heme Oxygenase-1 and Carbon Monoxide
- Neurological Disorders and Treatments
- Cancer-related Molecular Pathways
- Hippo pathway signaling and YAP/TAZ
- Blood groups and transfusion
- Protease and Inhibitor Mechanisms
- Immune cells in cancer
- MicroRNA in disease regulation
- Cardiac and Coronary Surgery Techniques
- Mesenchymal stem cell research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Complement system in diseases
Laboratoire Matière et Systèmes Complexes
2025
Inserm
2009-2023
Paris Cardiovascular Research Center
2013-2023
Université Paris Cité
2006-2023
Délégation Paris 5
2012-2018
Sorbonne Paris Cité
2012-2018
Hôpital Lariboisière
2005-2018
Assistance Publique – Hôpitaux de Paris
2013-2016
Hôpital Européen Georges-Pompidou
2009-2016
Hôpital Saint-Antoine
2008-2013
Objectives: Mesenchymal stem cells (MSCs) release extracellular vesicles (EVs) that display a therapeutic effect in inflammatory disease models. Although MSCs can prevent arthritis, the role of MSCs-derived EVs has never been reported rheumatoid arthritis. This prompted us to compare function exosomes (Exos) and microparticles (MPs) isolated from investigate their immunomodulatory Methods: Exos MPs were by differential ultracentrifugation. Immunosuppressive effects or investigated on T B...
Background— Atherosclerosis is an immunoinflammatory disease; however, the key factors responsible for maintenance of immune regulation in a proinflammatory milieu are poorly understood. Methods and Results— Here, we show that milk fat globule-EGF factor 8 (Mfge8, also known as lactadherin) expressed normal atherosclerotic human arteries involved phagocytic clearance apoptotic cells by peritoneal macrophages. Disruption bone marrow–derived Mfge8 murine model atherosclerosis leads to...
The coagulation protease thrombin plays a critical role in hemostasis and exerts pro-inflammatory pro-fibrotic effects via proteolytic activation of the major receptor, protease-activated receptor-1 (PAR-1). Connective tissue growth factor (CTGF) is novel fibroblast mitogen also promotes extracellular matrix protein production. It selectively induced by transforming β (TGF-β) thought to be autocrine agent responsible for mediating its effects. CTGF up-regulated during repair fibrotic...
The inhibitor of apoptosis protein survivin has been implicated in both cell cycle control and resistance. To discriminate between these different roles, we used transgenic expression the skin as a model for proliferation, differentiation, apoptosis. Transgenic mice expressing under keratin-14 promoter developed normally, without histologic abnormalities or hair, epidermal hyperplasia, developmental basal suprabasal epidermis. Keratinocyte proliferation assessed conditions, after...
Objective— To study the role of Mer receptor tyrosine kinase (mertk) in atherosclerosis. Methods and Results— We irradiated reconstituted atherosclerosis-susceptible C57Bl/6 low-density lipoprotein receptor-deficient female mice ( ldlr −/− ) with either a mertk +/+ or (tyrosine kinase-defective mertk) bone marrow. The were put on high-fat diet for 8 15 weeks. Mertk deficiency led to increased accumulation apoptotic cells within lesions, promoted proinflammatory immune response, accelerated...
In hemolytic diseases, such as sickle cell disease (SCD), intravascular hemolysis results in the release of hemoglobin, heme, and heme-loaded membrane microvesicles bloodstream. Intravascular is thus associated with inflammation organ injury. Complement system can be activated by heme vitro. We investigated mechanisms which red blood (RBC) degradation products trigger complement activation vivo. kidney biopsies SCD nephropathy patients a mouse model SCD, we detected tissue deposits C3 C5b-9....
Abstract Photodynamic therapy is an emerging cancer treatment that particularly adapted for localized malignant tumor. The phototherapeutic agent generally injected in the bloodstream and circulates whole organism as a chemotherapeutic agent, but needs light triggering to induce therapeutic effects. We found one of responses vitro vivo cells photodynamic was massive production emission extracellular vesicles (EVs): only 1 hour after photo-activation, thousands per cell were emitted medium. A...
Thrombin is a multifunctional serine protease that has crucial role in blood coagulation. It also potent mesenchymal cell mitogen and chemoattractant might therefore have an important the recruitment local proliferation of cells at sites tissue injury. We hypothesized thrombin affect deposition connective proteins these by directly stimulating fibroblast procollagen production. To address this hypothesis, effect on production gene expression human foetal lung fibroblasts was assessed over 48...
We hypothesized that microparticles (MPs) released after ischemia are endogenous signals leading to postischemic vasculogenesis.MPs from mice ischemic hind-limb muscle were detected by electron microscopy 48 hours unilateral femoral artery ligation as vesicles of 0.1- 1-microm diameter. After isolation sequential centrifugation, flow cytometry analyses showed the annexin V(+) MP concentration was 3.5-fold higher in calves than control muscles (1392+/-406 versus 394+/-180 MPs per 1 mg;...
Arterial cardiovascular events are the leading cause of death in patients with JAK2V617F myeloproliferative neoplasms (MPNs). However, their mechanisms poorly understood. The high prevalence myocardial infarction without significant coronary stenosis or atherosclerosis MPNs suggests that vascular function is altered. consequences mutation on reactivity unknown. We observe here increased responses to vasoconstrictors arteries from Jak2V617F mice resulting a disturbed endothelial NO pathway...
Proangiogenic cell therapy based on administration of bone marrow-derived mononuclear cells (BMCs) or endothelial progenitor (EPCs) is now under investigation in humans for the treatment ischemic diseases. However, mechanisms leading to beneficial effects BMCs and EPCs remain unclear.BMC- CD34+-derived interacted with femoral arteries through SDF-1 CXCR4 signaling released nitric oxide (NO) via an synthase (eNOS)-dependent pathway. BMC-induced NO production promoted a marked vasodilation...
Sickle cell disease (SCD) is linked to hypercoagulability and characterised by high concentrations of erythrocyte-derived microparticles (Ed-MPs). However, the impact procoagulant cell-derived on thrombin generation process remains unclear. We analysed alterations each phase (TG) in relation concentration erythrocyte- or platelet-derived (Ed-MPs Pd-MPs) a cohort patients with steady-state SCD. studied 92 SCD patients, 19 which were under treatment hydroxyurea, 30 healthy age- sex-matched...
Objective— Several secreted phospholipases A2 (sPLA2s), including group IIA, III, V, and X, have been linked to the development of atherosclerosis, which led clinical testing A-002 (varespladib), a broad sPLA2 inhibitor for treatment coronary artery disease. Group X (PLA2G10) has most potent hydrolyzing activity toward phosphatidylcholine is believed play proatherogenic role. Methods Results— Here, we show that Ldlr –/– mice reconstituted with bone marrow from mouse X–deficient ( Pla2g10 )...
Intravascular erythrocyte destruction, accompanied by the release of pro-oxidative and pro-inflammatory components hemoglobin heme, is a common event in pathogenesis numerous diseases with heterogeneous etiology clinical features. A frequent adverse effect related to massive hemolysis renal injury inflammation. Nevertheless, it still unclear whether heme – danger-associated molecular pattern ligand for TLR4 or upstream hemolysis-derived products are responsible these effects....