A5069087828- Mesenchymal stem cell research
- Immune cells in cancer
- Osteoarthritis Treatment and Mechanisms
- Bone and Joint Diseases
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Zebrafish Biomedical Research Applications
- Mitochondrial Function and Pathology
- Cardiac Ischemia and Reperfusion
- CAR-T cell therapy research
- Cancer Cells and Metastasis
- Periodontal Regeneration and Treatments
- Autophagy in Disease and Therapy
- RNA Interference and Gene Delivery
- Phagocytosis and Immune Regulation
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- Aquaculture disease management and microbiota
- Peroxisome Proliferator-Activated Receptors
- Tissue Engineering and Regenerative Medicine
- Cardiac Fibrosis and Remodeling
- Wound Healing and Treatments
- Cancer-related cognitive impairment studies
- Immune Cell Function and Interaction
- Cancer, Hypoxia, and Metabolism
Universidad de Los Andes, Chile
2016-2025
Advanced Center for Chronic Diseases
2024
Universidad de Los Andes
2020-2024
Institute for Regenerative Medicine & Biotherapy
2015-2021
Centro de Investigación Biomédica en Red
2020
Medicina
2020
Inserm
2012-2018
Université de Montpellier
2014-2018
Hôpital Saint Eloi
2015
Centre Hospitalier Universitaire de Montpellier
2015
Abstract Introduction Mesenchymal stem cells (MSCs) are adult, multipotent, with immunomodulatory properties. The mechanisms involved in the capacity of MSCs to inhibit proliferation proinflammatory T lymphocytes, which appear responsible for causing autoimmune disease, have yet be fully elucidated. One underlying studied recently is ability generate regulatory (Treg) vitro and vivo from activated peripheral blood mononuclear (PBMC), T-CD4+ also T-CD8+ cells. In present work we investigated...
Objectives: Mesenchymal stem cells (MSCs) release extracellular vesicles (EVs) that display a therapeutic effect in inflammatory disease models. Although MSCs can prevent arthritis, the role of MSCs-derived EVs has never been reported rheumatoid arthritis. This prompted us to compare function exosomes (Exos) and microparticles (MPs) isolated from investigate their immunomodulatory Methods: Exos MPs were by differential ultracentrifugation. Immunosuppressive effects or investigated on T B...
While the mammalian macrophage phenotypes have been intensively studied in vitro, dynamic of their phenotypic polarization has never investigated live vertebrates. We used zebrafish as a model to identify and trail subtypes. generated transgenic line whose macrophages expressing tumour necrosis factor alpha (tnfa), key feature classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding...
Abstract The role of interleukin 1 receptor antagonist (IL1RA) in mediating the immunosuppressive effect mesenchymal stem/stromal cells (MSCs) has been reported several studies. However, how MSC-derived IL1RA influences host response not clearly investigated. We therefore derived MSCs from bone marrow knockout mice and evaluated their on different immune cell subsets. deficient (IL1RA−/−) or wild type (wt) inhibited to same extend proliferation T lymphocytes. On contrary, IL1RA−/− were less...
Abstract Osteoarthritis (OA) is the most common degenerative joint disease. Mesenchymal stromal cells (MSC) are promising cell-based therapy for OA. However, there still a need additional randomized, dose-dependent studies to determine optimal dose and tissue source of MSC improved clinical outcomes. Here, we performed dose-dependant evaluation umbilical cord (UC)-derived (Celllistem) in murine model knee OA patients. For preclinical study, classical (200.000 cells) lower (50.000 Cellistem...
Osteoarthritis (OA) is a joint disease characterized by articular cartilage degradation. Persistent low-grade inflammation defines OA pathogenesis, with crucial involvement of pro-inflammatory M1-like macrophages. While mesenchymal stromal cells (MSC) and their small extracellular vesicles (sEV) hold promise for treatment, achieving consistent clinical-grade sEV products remains significant challenge. This study aims to develop fully characterized, reproducible, batches derived from...
MSC display potent suppressive properties initially described a decade ago. More recently, activities on T-cell effector pathways have been investigated. modulate CD4 differentiation through different mechanisms depending culture conditions and disparate T cells according to their status. A significant amount of evidence for effects Th17 revealed that could be under diverse circumstances but also enhance cell activity other conditions. In the present study, we investigated Th1 subsets using...
Abstract Macrophages are essential for appendage regeneration after amputation in regenerative species. The molecular mechanisms through which macrophages orchestrate blastema formation and still unclear. Here, we use the genetically tractable transparent zebrafish larvae to study functions of polarized macrophage subsets during caudal fin regeneration. After amputation, show an early transient accumulation pro-inflammatory concomitant with non-inflammatory which, contrast macrophages,...
Stem cells isolated from menstrual fluid (MenSCs) exhibit mesenchymal stem cell (MSCs)-like properties including multi-lineage differentiation capacity. Besides, has important advantages over other sources for the isolation of MSCs, ease access and repeated sampling in a noninvasive manner. Such attributes allow rapid culture MenSCs numbers that are sufficient therapeutical doses, at lower passages.In this study, we advance characterization MenSC populations comparison to bone marrow derived...
Mesenchymal stem cells (MSCs) are multipotent with broad immunosuppressive capacities. Recently, it has been reported that MSCs can transfer mitochondria to various cell types, including fibroblast, cancer, and endothelial cells. It suggested mitochondrial is associated a physiological response cues released by damaged restore regenerate tissue. However, the role of immune competent poorly investigated. Here, we analyzed capacity from bone marrow (BM) healthy donors (BM-MSCs) primary...
Recently, a noninvasive and highly proliferative stem cell population from menstrual blood called MenSCs has been identified. Despite their use in clinical studies, immunomodulatory properties have not yet investigated. In this context, we studied the immunosuppressive of comparison with well-characterized bone marrow derived-MSCs (BM-MSCs). Using an vitro proliferation assays, showed that displayed lower suppressive effect on peripheral mononuclear cells particular proinflammatory CD4(+)...
Background Application of mesenchymal stem/stromal cells (MSCs) in treating different disorders, particular osteo-articular diseases, is currently under investigation. We have already documented the safety administrating human adipose tissue-derived stromal MSCs (hASCs) immunodeficient mice. In present study, we investigated whether persistence MSC affected by degree inflammation and related to therapeutic effect two inflammatory models arthritis. Methodology/Principal Findings used C57BL/6...
Abstract Background Mesenchymal stem cells (MSCs) have been recognized for their regenerative and anti-inflammatory capacity which makes them very attractive to cell therapy, especially those ones treat inflammatory autoimmune disease. Two different immune-phenotypes described MSCs depending on Toll-like receptor (TLR) is activated. MSC1 endowed with a pro-inflammatory phenotype following TLR4 activation LPS. On the other hand, MSC2 induced by of TLR3 Poly(I:C). High immunoplasticity matter...
Hypoxia-inducible factor 1 α (HIF1α), a regulator of metabolic change, is required for the survival and differentiation potential mesenchymal stem/stromal cells (MSC). Its role in MSC immunoregulatory activity, however, has not been completely elucidated. In present study, we evaluate HIF1α on immunosuppressive potential. We show that silencing decreases their inhibitory Th1 Th17 cell generation limits capacity to generate regulatory T cells. This reduced associated with switch from...
Osteoarthrosis (OA) is a leading cause of disability and early mortality, with no disease modifying treatment. Mitochondrial (MT) dysfunction changes in energy metabolism, to oxidative stress apoptosis, are main drivers disease. In reaction stress, mesenchymal stromal/stem cells (MSCs) donate their MT damaged tissues.
The inflammatory responses from synovial fibroblasts and macrophages the mitochondrial dysfunction in chondrocytes lead to oxidative stress, disrupt extracellular matrix (ECM) homeostasis, accelerate deterioration process of articular cartilage osteoarthritis (OA). In recent years, it has been proposed that mesenchymal stromal cells (MSC) transfer their functional mitochondria damaged response cellular becoming one mechanisms underpinning therapeutic effects. Therefore, we hypothesize a...
To define how peroxisome proliferator-activated receptor (PPAR) β/δ expression level in mesenchymal stem cells (MSCs) could predict and direct both their immunosuppressive therapeutic properties. PPARβ/δ interacts with factors such as nuclear factor-kappa B (NF-κB) regulates the of molecules including vascular cell adhesion molecule (VCAM)-1 intercellular (ICAM)-1. Since these are critical for MSC function, we investigated role on properties.We either treated human MSCs (hMSCs) irreversible...
Objective Mesenchymal stem cells (MSCs) are potent immunosuppressive that have shown promise in the treatment of rheumatoid arthritis (RA). Deciphering intrinsic characteristics MSCs correlate with their biologic activity will facilitate clinical use. Recently, role glucocorticoid‐induced leucine zipper (GILZ) development RA has been documented. The aim this study was to evaluate whether GILZ expression by may contribute therapeutic effect. Methods were isolated from GILZ‐deficient (GILZ −/−...