A5038400715- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
- Cancer-related Molecular Pathways
- TGF-β signaling in diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- RNA modifications and cancer
- Genetic factors in colorectal cancer
- MicroRNA in disease regulation
- Lipid metabolism and disorders
- Medical Imaging and Pathology Studies
- Cancer-related molecular mechanisms research
- Environmental DNA in Biodiversity Studies
- Metabolism, Diabetes, and Cancer
- Ubiquitin and proteasome pathways
- Ferroptosis and cancer prognosis
- Radiomics and Machine Learning in Medical Imaging
- Extracellular vesicles in disease
- Pharmaceutical and Antibiotic Environmental Impacts
- Parathyroid Disorders and Treatments
- Microbial Community Ecology and Physiology
- Otitis Media and Relapsing Polychondritis
- Congenital Ear and Nasal Anomalies
- Cancer Diagnosis and Treatment
- IL-33, ST2, and ILC Pathways
- Antimicrobial Peptides and Activities
Tongji University
2008-2024
Tongji Hospital
2014-2024
Shanghai Pulmonary Hospital
2008
// Xiaoye Liu 1,2,3,* , Xiaoting Yu 4,* Jingjing Xie 5 Mengna Zhan 6 Zhuo 3 Li Hongxiang Zeng Feifei Zhang Guoqiang Chen 1,3 Xianghua Yi 4 and Junke Zheng 2,3 1 Institute of Health Sciences, Shanghai for Biological University Chinese Academy Science, Sciences Jiao Tong School Medicine, Shanghai, China 2 Hongqiao International Tongren Hospital, Faculty Basic Key Laboratory Cell Differentiation Apoptosis Ministry Education, Department Pathology, Tongji Bingzhou Medical University, Taishan...
Abstract Obesity increases the risk of distant metastatic recurrence and reduces breast cancer survival. However, mechanisms behind this pathology identification relevant therapeutic targets are poorly defined. Plasma free fatty acids (FFA) levels elevated in obese individuals. Here we report that TGFβ transiently activates ERK subsequently phosphorylates SMAD4 at Thr277, which facilitates a SMAD4–USP9x interaction, nuclear retention, stimulates TGFβ/SMAD3–mediated transcription Twist Snail....
The aim of this study was to identify potential microRNAs and genes associated with idiopathic pulmonary fibrosis (IPF) through web-available microarrays. microRNA microarray dataset GSE32538 the mRNA datasets GSE32537, GSE53845, GSE10667 were downloaded from Gene Expression Omnibus (GEO) database. Differentially expressed miRNAs (DE-miRNAs)/genes (DEGs) screened GEO2R, their associations IPF analyzed by comprehensive bioinformatic analyses. A total 45 DE-microRNAs identified between control...
Increased expression of Pituitary Tumor Transforming Gene 1 (Pttg1) has been shown in various tumor cells, including breast cancer (BC). However, the precise role Pttg1 tumorigenesis is not clarified yet. Here, we examined BC from patients and detected significant increases correlation phosphorylated SMAD3 (pSMAD3), a key effector activated transforming growth factor β (TGFβ) receptor signaling pathway. levels were then modulated by transgene or small hairpin RNA (shRNA) human cell line,...
Epidemiologic evidence indicates that diabetes may increase risk of breast cancer (BC) and mortality in patients with cancer. The pathophysiological relationships between are not fully understood, personalized treatments for diabetes-associated BC urgently needed.
Objective: Idiopathic interstitial pneumonias (IIPs) are a group of diffuse parenchymal lung diseases unknown etiology characterized by the presence various degrees inflammation and fibrosis. We aimed to screen differences among IIPs subtypes in gene level using microarray expression profiles normal tissue for key genes associated with early diagnosis treatment IIPs. Methods: The profile six kinds (GSE 32537) tissues were downloaded. differentially expressed (DEGs) different selected...
BACKGROUND:Clinical cases of nonmedullary thyroid carcinoma (NMTC) in combination with primary hyperparathyroidism (PHPT) have been reported occasionally. However, the clinical characteristics and risk factors concomitant NMTC PHPT patients remain unclear. This study aimed to assess association between NMTC, evaluate Chinese PHPT. MATERIAL AND METHODS:This was a retrospective cohort analysis. We reviewed medical records 155 who underwent surgery for two large centers China 2009 2014. The...
The purpose of this work was to explore the systematic molecular mechanism lung adenocarcinoma and gain a deeper insight into it.Comprehensive bioinformatics methods were applied. Initially, significant differentially expressed genes (DEGs) analyzed from Affymetrix microarray data (GSE27262) deposited in Gene Expression Omnibus (GEO). Subsequently, gene ontology (GO) analysis performed using online Database for Annotation, Visualization Integration Discovery (DAVID) software. Finally,...
Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive lung disease with limited effective therapies. Interstitial macrophages (IMs), especially those derived from monocytes, play an unknown role in IPF pathogenesis. By using single-cell RNA sequencing (scRNA-seq), bleomycin (BLM)-induced mouse lungs were analyzed to characterize the cellular landscape and heterogeneity of this model. scRNA-seq was used identify distinct interstitial macrophage subpopulations fibrotic lungs,...
Expression of miR-106 in endometrial carcinoma RL95-2 cell line and its effect on proliferation invasion cancer cells was investigated. expression vector constructed transiently transfected into vitro cultured human carcinoma. Cells were divided three groups including blank control (MOCK group), transfection group (miR-106 group) negative (siNC group). Reverse-transcription quantitative PCR (RT-qPCR) used to detect the miR-106. Proliferation migration detected by MTT scratch assay, apoptosis...
The aim of this study was to identify the molecular events that distinguish serrated colorectal carcinoma (SCRC) from conventional (CCRC) through differential gene expression, pathway and protein-protein interaction (PPI) network analysis. GSE4045 GSE8671 microarray datasets were downloaded Gene Expression Omnibus database. We identified genes are differentially expressed between SCRC normal colon tissues, CCRC healthy using Student's t-tests Benjamini‑Hochberg (BH) multiple testing...
<p>Figure S6 The comparison of expression levels phospho-ERK and phospho-Smad4 between lean overweight/obesity patients, Related to Figure 7. Staining was analyzed by using a system based on the percentage positively stained cells staining intensity. Integrated optical density all positive in each image determined, its ratio total area photograph calculated as density.</p>
<p>Figure S3 FFA promotes TGF-beta-induced migration by activating ERK and USP9x, Related to Figure 3. A, BT549 cells were plated in Ibidi culture insert dishes (Madison, WI). Cells cultured complete medium 80% confluence, inserts removed create a gap then incubated with BSA, PA, PA plus AZD6244 or WP1130 the absence presence of TGF-beta1. Migrating photographed under phase contrast microscope. B, The percentage wound closed was quantified from three independent replicates is expressed...
<div>Abstract<p>Obesity increases the risk of distant metastatic recurrence and reduces breast cancer survival. However, mechanisms behind this pathology identification relevant therapeutic targets are poorly defined. Plasma free fatty acids (FFA) levels elevated in obese individuals. Here we report that TGFβ transiently activates ERK subsequently phosphorylates SMAD4 at Thr277, which facilitates a SMAD4–USP9x interaction, nuclear retention, stimulates TGFβ/SMAD3–mediated...
<div>Abstract<p>Obesity increases the risk of distant metastatic recurrence and reduces breast cancer survival. However, mechanisms behind this pathology identification relevant therapeutic targets are poorly defined. Plasma free fatty acids (FFA) levels elevated in obese individuals. Here we report that TGFβ transiently activates ERK subsequently phosphorylates SMAD4 at Thr277, which facilitates a SMAD4–USP9x interaction, nuclear retention, stimulates TGFβ/SMAD3–mediated...
<p>Figure S2 A, ERK activation is responsible for FFA promotion of TGF-beta-induced inhibition SMAD4 monoubiquitination, Related to Figure 2. MDA-MB-231 cells were treated with BSA (vehicle) or PA in the presence absence AZD6244 WP1130 4 h followed by treatment without 3 ng/ml TGF-beta another h. Nuclear extracts made and monoubiquitination was detected. The blot a representative blots from independent experiments. *P<0.01 vs control; #P<0.05, BSA; $P<0.01, PA+AZD PA, PA+WP...
<p>Figure S6 The comparison of expression levels phospho-ERK and phospho-Smad4 between lean overweight/obesity patients, Related to Figure 7. Staining was analyzed by using a system based on the percentage positively stained cells staining intensity. Integrated optical density all positive in each image determined, its ratio total area photograph calculated as density.</p>
<p>Figure S4 Quantification of Western blot analysis (related to Figure 4) (B-G). Data are presented as mean fold increases ({plus minus}SD) in treated groups over basal values from three independent experiments. B, *p<0.01 vs BSA; #p<0.01 PA+AZD PA. E, EV/-PA; EV/+PA. F, EV/-TGF-beta-PA; #p<0.05 EV/+TGF-beta-PA; G, WT.</p>