A5076442788- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- 3D Printing in Biomedical Research
- Veterinary Medicine and Surgery
- Cardiac Fibrosis and Remodeling
- Photochromic and Fluorescence Chemistry
- Congenital heart defects research
- Supramolecular Self-Assembly in Materials
- Animal testing and alternatives
- Renal and Vascular Pathologies
- COVID-19 Clinical Research Studies
- Diet and metabolism studies
- Biomedical Ethics and Regulation
- SARS-CoV-2 and COVID-19 Research
- RNA Interference and Gene Delivery
- Bone Tissue Engineering Materials
- Cardiovascular Function and Risk Factors
- Muscle metabolism and nutrition
- Peptidase Inhibition and Analysis
- Cellular Mechanics and Interactions
- Chronic Kidney Disease and Diabetes
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2017-2025
University Medical Center Groningen
2017-2025
University of Groningen
2017-2025
Universidade Estadual Paulista (Unesp)
2018-2020
Decellularized-organ-derived extracellular matrix (dECM) has been used for many years in tissue engineering and regenerative medicine. The manufacturing of hydrogels from dECM allows to make use the pro-regenerative properties ECM and, simultaneously, shape material any necessary way. objective present project was investigate differences between cardiovascular tissues (left ventricle, mitral valve, aorta) with respect generating their interaction cells 2D 3D. left aorta porcine hearts were...
Abstract Transforming growth factor-β1 (TGF-β1) is a potent inducer of fibroblast to myofibroblast differentiation and contributes the pro-fibrotic microenvironment during cardiac remodeling. Fibroblast factor-2 (FGF-2) factor secreted by adipose tissue-derived stromal cells (ASC) which can antagonize TGF-β1 signaling. We hypothesized that TGF-β1-induced abrogated FGF-2 ASC conditioned medium (ASC-CMed). Our experiments demonstrated treatment-induced into myofibroblasts, as evidenced...
Progenitor cells cultured on biomaterials with optimal physical-topographical properties respond alignment and differentiation. Stromal from connective tissue can adversely differentiate to profibrotic myofibroblasts or favorably smooth muscle (SMC). We hypothesized that myogenic differentiation of adipose tissue-derived stromal (ASC) depends gradient directional topographic features. Polydimethylsiloxane (PDMS) samples nanometer micrometer topography gradients (wavelength (w) = 464-10, 990...
Animal experimentation requires a solid and rational moral foundation. Objective emphatic decision-making protocol evaluation by researchers ethics committees remain difficult sensitive matter. This article presents three perspectives that facilitate consideration of the minimally acceptable standard for animal experiments, in particular, tissue engineering (TE) regenerative medicine. First, we review boundaries provided law public opinion America Europe. Second, contemporary theory to...
The administration of trophic factors (TFs) released by mesenchymal stromal cells (MSCs) as therapy for cardiovascular diseases requires a delivery vehicle capable binding and releasing the TF in sustained manner. We hypothesized that hydrogels derived from cardiac decellularized extracellular matrix (cardiac dECM) bind MSC secretome-derived release these fashion. Pig-derived ventricular tissue was decellularized, milled to powder, digested, assembled hydrogel upon warming at 37 °C....
Doxorubicin-induced cardiomyopathy (DOX-IC) is a significant and common complication in patients undergoing chemotherapy, leading to cardiac remodeling reduced heart function. We hypothesized that the intrapericardial injection of hydrogels derived from decellularized extracellular matrix (dECM) loaded with adipose tissue-derived stromal cells (ASC) their secretome dampens or reverses progression DOX-IC. DOX-IC was induced Wistar male rats through ten weekly intra-peritoneal injections...
Abstract Objectives Endothelial cells undergo TGF‐β–driven endothelial‐mesenchymal transition (EndMT), representing up to 25% of cardiac myofibroblasts in ischaemic hearts. Previous research showed that conditioned medium adipose tissue–derived stromal (ASC‐CMed) blocks the activation fibroblasts into fibrotic myofibroblasts. We tested hypothesis ASC‐CMed abrogates EndMT and prevents formation adverse Materials methods Human umbilical vein endothelial (HUVEC) were treated with IL‐1β TGF‐β2...
COVID-19, along with most respiratory diseases in the medical field, demonstrates significant ability to take its toll on global population. There is a particular difficulty studying these conditions, which stems especially from short supply of vitro models for detailed investigation, specific therapeutic knowledge required disease scrutinization and occasional need BSL-3 [Biosafety Level 3] laboratories research. Based this, process drug development hampered great extent. In scenario this...
ABSTRACT: The objective was to verify the effectiveness of ketoanalogues in dogs with Chronic Kidney Disease (CKD) stage 3. Controlled randomized clinical trial performed twenty CKD Animals were subjected to: Group 1 (control): conventional therapy (CT) for CKD; 2: CT and 60mg/kg, OA, q48h keto-supplementa; 3, q24h 4, 120mg/kg, q12h keto-supplementa. All received canine renal diet. evaluated at beginning after 15 30 days. Complete blood count (CBC), serum urea, creatinine, phosphorus,...
Background: Chronic kidney disease (CKD) affects both dogs and cats, mainly elderly animals, due to tubulointerstitial inflammation associated with the increase of fibrosis through excess deposition extracellular matrix (ECM) which leads decrease glomerular filtration. Many different underlying renal diseases can affect kidneys such as congenital or acquired in origin. Therefore, main objective this transversal study was evaluate epidemiology clinical laboratory evaluation 225 client-owned...