A5007387330- Aquaculture disease management and microbiota
- Aquaculture Nutrition and Growth
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Dendrimers and Hyperbranched Polymers
- Supramolecular Chemistry and Complexes
- interferon and immune responses
- Computational Drug Discovery Methods
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Viral Infections and Vectors
- Advanced DC-DC Converters
- Animal Virus Infections Studies
- Multilevel Inverters and Converters
- Environmental Toxicology and Ecotoxicology
- Chromosomal and Genetic Variations
- Microgrid Control and Optimization
- Mosquito-borne diseases and control
- Aquatic Ecosystems and Phytoplankton Dynamics
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- RNA modifications and cancer
- Metal-Organic Frameworks: Synthesis and Applications
- X-ray Diffraction in Crystallography
- Invertebrate Immune Response Mechanisms
Chinese Academy of Fishery Sciences
2019-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2016-2025
Ministry of Agriculture and Rural Affairs
2021-2025
Ministère de l'Agriculture et de la Souveraineté alimentaire
2024
Yanshan University
2022-2023
Shantou University
2023
Academy of Medical Sciences
2020
California Institute of Technology
2015-2019
Beijing Normal University
2011-2019
Guangdong University of Technology
2018
Antiviral therapeutics is one effective avenue to control and end this devastating COVID-19 pandemic. The viral RNA-dependent RNA polymerase (RdRp) of SARS-CoV-2 has been recognized as a valuable target antivirals. However, the cell-free RdRp biochemical assay requires conversion nucleotide prodrugs into active triphosphate forms, which regularly occurs in cells yet complicated multiple-step chemical process vitro, thus hinders utility rapid discovery inhibitors. In addition, exoribonuclease...
Abstract The emergence of new highly pathogenic and drug-resistant influenza strains urges the development novel therapeutics for A virus (IAV). Here, we report discovery an anti-IAV microbial metabolite called APL-16-5 that was originally isolated from plant endophytic fungus Aspergillus sp. CPCC 400735. binds to both E3 ligase TRIM25 IAV polymerase subunit PA, leading ubiquitination PA subsequent degradation in proteasome. This mode action conforms a proteolysis targeting chimera which...
The productive infection of influenza A virus (IAV) depends on host factors. However, the involvement long non-coding RNAs (lncRNAs) in IAV remains largely uninvestigated. In this work, we have discovered a human lncRNA, named lncRNA-PAAN (PA-associated noncoding RNA) that enhances replication. level increases upon IAV, but not other viruses, nor interferon treatment, suggesting specific up-regulation expression by IAV. Silencing significantly decreases replication through impairing activity...
Long noncoding RNAs (lncRNAs) participate in host antiviral defense by modulating immune responses. However, it remains largely unexplored how viruses exploit interferon (IFN)-independent lncRNAs to facilitate viral replication. Here, we have identified a group of human that modulate influenza A virus (IAV) replication loss-of-function screen and found an IFN-independent lncRNA, called IPAN, is hijacked IAV assist IPAN specifically induced infection independently IFN associates with...
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has become one major threat to human population health. The RNA-dependent RNA polymerase (RdRp) presents an ideal target of antivirals, whereas nucleoside analogs inhibitor is hindered by the proofreading activity coronavirus. Herein, we report that corilagin (RAI-S-37) as a non-nucleoside SARS-CoV-2 RdRp, binds directly effectively inhibits in both cell-free and cell-based assays, fully resists potently infection with low 50%...
Coronavirus disease 2019 (COVID-19) is a fatal respiratory illness caused by severe acute syndrome coronavirus 2 (SARS-CoV-2). The identification of potential drugs urgently needed to control the pandemic. RNA dependent polymerase (RdRp) conserved protein within viruses and plays crucial role in viral life cycle, thus making it an attractive target for development antiviral drugs. In this study, 101 quinoline quinazoline derivatives were screened against SARS-CoV-2 RdRp using cell-based...
Prometryn, a triazine pesticide product used to control weed growth, poses high risk aquatic organisms in the environment. Several toxicological evaluations have been performed on bony fish and shrimp exposed prometryn. However, there no reports conducted toxic mechanism of prometryn with regard Eriocheir sinensis. In this study, our research evaluated effects via vitro vivo toxicity tests E. Firstly, we estimated exposure sinensis, then constructed 6 h transcriptional profile an enrichment...
In China, the in-pond raceway system (IPRS) recirculating culture is a new model of freshwater aquaculture. A 120-day feeding study was carried out to evaluate influence stocking density on growth, digestive enzymes activity, serum parameters, antioxidant status, liver and intestine histology gene expression HSP70 Cu/Zn-SOD largemouth bass farmed in IPRS. Largemouth bass, Micropterus salmoides (initial body weight, 4.50 ± 0.23 g), were randomly nine raceways stocked at three densities 0.2...
Type I interferon (IFN) inhibits viruses by inducing the expression of antiviral proteins. The IFN-induced myxovirus resistance B (MxB) protein has been reported to inhibit a limited number viruses, including HIV-1 and herpesviruses, but its coverage remains be explored further. Here we show that MxB interferes with RNA replication hepatitis C virus (HCV) significantly viral in cyclophilin A (CypA)-dependent manner. Our data further interacts HCV NS5A, thereby impairing NS5A interaction CypA...
Salvianolic acid B (Sal B), as one of the main water-soluble components Salvia miltiorrhizae, has significant pharmacological activities, including antioxidant, free radical elimination and biofilm protection actions. However, protective effect Sal on Nile tilapia underlying mechanism are rarely reported. Therefore, aim this study was to evaluate effects antioxidant stress, apoptosis autophagy in liver. In experiment, were fed diets containing sal (0.25, 0.50 0.75 g·kg−1) for 60 days, then...
To investigate the potential toxic mechanism of Microcystin-LR (MC-LR) on juvenile Eriocheir sinensis (E. sinensis), a toxicity test was conducted by injecting MC-LR into 3rd pereiopod. Following injection, we evaluated alterations in immune response biomarkers, antioxidant status, lipid metabolism-related genes expression, and intestinal microbiota composition hepatopancreas tissues E. sinensis. The results showed that after injection MC-LR, serum complement C3 level significantly decreased...
Type I interferon (IFN) inhibits a wide spectrum of viruses through stimulating the expression antiviral proteins. As an IFN-induced protein, myxovirus resistance B (MXB) protein was reported to inhibit multiple highly pathogenic human viruses. It remains be determined whether MXB employs common mechanism restrict different Here, we find that IFN alters subcellular localization hundreds host proteins, and this effect is partially lost upon depletion. The results our mechanistic study reveal...
Abstract Background Seasonal migration is one of the most spectacular events in nature; however, molecular mechanisms related to this phenomenon have not been investigated detail. The Chinese tapertail, or Japanese grenadier anchovy, Coilia nasus, a valuable migratory fish high economic importance and special dimorphism (with certain individuals as non-migratory residents). Results In study, an 870.0-Mb high-quality genome was assembled by combination Illumina Pacific Biosciences sequencing....
Coronavirus disease 2019 (COVID-19) is a newly emerged infectious caused by novel coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The rapid global emergence of SARS-CoV-2 highlights importance and urgency for potential drugs to control pandemic. functional RNA-dependent RNA polymerase (RdRp) in viral life cycle, combined with structural conservation absence closely related homologs humans, makes it an attractive target designing antiviral drugs. Nucleos(t)ide...
The construction of a new family endo-functionalized multiferrocenyl hexagons with various sizes via coordination-driven self-assembly is described. structures these novel metallacycles, containing several ferrocenyl moieties at their interior surface, are characterized by multinuclear NMR ((31)P and (1)H) spectroscopy, cold-spray ionization mass spectrometry (CSI-TOF-MS), elemental analysis, molecular modeling. Insight into the structural electrochemical properties was obtained through...
Ebola virus (EBOV) infection leads to staggeringly high mortality rate. Effective and low-cost treatments are urgently needed control frequent EBOV outbreaks in Africa. In this study, we report that a natural compound called berbamine hydrochloride strongly inhibits replication vitro vivo. Our work further showed acts by directly binding the cleaved glycoprotein (GPcl), disrupting GPcl interaction with viral receptor Niemann-Pick C1, thus blocking fusion of cellular membranes. data support...
There is growing evidence that long-term exposure to prometryn (a widely used herbicide) can induce toxicity in bony fish and shrimp. Our previous study demonstrated its 96 h acute on the crab Eriocheir sinensis. However, studies whether longer with a lower dose induces E. sinensis are scarce. Therefore, we conducted 20 d experiment investigate effects hepatopancreas intestine of sinensi. Prometryn reduce activities antioxidant enzymes, increase level lipid peroxidation cause oxidative...
Recently, probiotics have been widely applied for the in situ remediation of aquatic water. Numerous studies proved that can regulate water quality by improving microbial community. Nitrogen cycling, induced microorganisms, is a crucial process maintaining balance ecosystem. Nevertheless, underlying mechanisms which enhance systems remain poorly understood. To explore indicators and their correlation with nitrogen cycling-related functional genes, metagenomic analysis element cycling was...
This study employed multi-omics analysis to systematically evaluate the toxic effects of intraperitoneal injection MC-LR on GIFT tilapia. The results showed that 96 h post injection, serum levels aspartate GOT, GPT, LYZ, T-AOC, and SOD significantly decreased (p < 0.05). In contrast, hepatic CAT MDA increased. 16S rDNA sequencing method revealed a significant reduction in α diversity intestinal microbiota. At phylum level, relative abundances Firmicutes Bacteroidota decreased; at genus...
African swine fever is a highly lethal disease caused by the virus (ASFV), posing significant threat to global pig industry, wherease no approved treatments are currently available. The ASFV DNA-binding protein, pA104R, plays critical role in viral genome packaging and replication, making it key target for drug discovery. Through structure-based virtual screening, we identified polyphenolic compound, thonningianin A, which disrupts pA104R − DNA binding significantly inhibits replication....
LINE-1 is the only active autonomous mobile element in human, and its mobilization tightly restricted by host to maintain genetic stability. We recently reported that human MOV10 recruits DCP2 decap RNA liquid-liquid phase separation (LLPS), resulting inhibition of retrotransposition, while detailed mechanism still awaits further exploration. In this report, we found extended motif II (563–675aa) C-terminal domain (907–1003aa) cooperated achieve maximal on retrotransposition. The involves...