A5013089120- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Additive Manufacturing and 3D Printing Technologies
- Cardiac Valve Diseases and Treatments
- Infective Endocarditis Diagnosis and Management
- Aortic Disease and Treatment Approaches
- Engineering Education and Technology
- Cardiac and Coronary Surgery Techniques
- Xenotransplantation and immune response
- Bone Tissue Engineering Materials
- Cellular and Composite Structures
- Technology Assessment and Management
Kyiv City Clinical Oncology Center
2021
Ministry of Health
2020
Ukrainian Museum
2020
The rapid growth of cardiovascular morbidity and high mortality rates patients with congenital heart disease requiring surgery have led to the search for new modern approaches treatment these groups patients. main trends today include use cardiaс implants synthetic biological origin. Of particular interest are scaffolds based on decellularized extracellular matrix, which in its functional structural characteristics is close native pericardium. In contrast analogues, such grafts can fully...
Aim. Determination of the most effective protocol for decellularization bovine pericardium.Methods.Bovine pericardium was decellularized following five protocols: group 1 -Ammonium Hydroxide + Triton X-100, 2 -1 % SDS Ammonium -Triton 3 SDS, 4 -Trypsin enzyme 5 -0,1 respectively.Histological, biomechanical properties and detection nucleic acids concentration in samples were studied.Results.The time groups is much longer than described previous scientific publication.Also, lower control...
The decellularized bovine pericardium and its potential use as a natural scaffold is promising approach in the field of tissue engineering regenerative medicine. reaction host toward scaffolds depends on their biocompatibility, which should be satisfied being before applied clinical use. Purpose: to evaluate biocompatibility extracellular matrices, were by trypsin enzyme anionic sodium dodecyl sulfate (SDS) detergent. Material methods. Pericardial sacs acquired from 12-18 months’ age bulls....
Patients with congenital heart defects and cardiovascular diseases are required new approaches to surgical intervention. The use of biological cardiac implants, which made from the extracellular matrix, is a promising trend in modern regenerative medicine. These bioimplants can completely replace defective tissue or organs, when manufactured strict protocols quality control measures, be safe effective for therapeutic applications. process manufacturing involves various risks that need...
Background. In modern cardiovasclar surgery, it is a promising method to use xenotissues, which in their properties are close human tissues, order restore the integrity of heart chambers, its walls or valves. Decellularization extracellular matrix (EMC) applied process creation such bioprotheses. EMC, elastin and collagen components preserved, antigenic molecules eliminated resulting reduction risk rejection. The study devoted assessment histological, molecular-genetic cytotoxic...
The aim: To investigate the effectiveness of using low concentrations sodium dodecyl sulfate (SDS) and cross-linking with EDC/NHS in decellularization process to create a potential bioimplant for cardiac surgery. Materials methods: Pericardial sacs were derived from 12-18 months bulls. Tissue was performed by 0.1% SDS following cross-linking. experiment included standard histological, microscopic, molecular genetic biomechanical methods. Scaffold tested vitro cytotoxicity biocompatibility....
The aim of the\* study was to estimate the properties scaffold obtained by decellularization bovine pericardium with a 0.1% solution sodium dodecyl sulfate. experiment included standard histological, microscopic, molecular genetic, and biomechanical methods. Scaffold tested in vitro for cytotoxicity vivo biocompatibility. A high degree removal cells their components from pericardium-derived matrix shown. Biomechanical characteristics artificial were same as those native pericardium. With...
The use of xenotissue for the needs regenerative and cardiovasculare medicine is a promising area tissue engineering.The decellularization process provides complete purification elastin-collagen matrix bovine pericardium from cells their components.The high concentrations sodium dodecyl sulfate glutaraldehyde can lead to damage extracellular matrix.Therefore, purpose this study was microarchitectonics decellularized using low concentration (0.1% solution) avoiding glutaraldehyde.Further...

 Background. Recent developments in the field of transcatheter aortic valve implantation (TAVI) have led to arised interest for optimization this procedure and related devices patients with severe stenosis. This is first study examine novel XPand device, jointly developed by German, French Ukrainian scientists. aims contribute growing area research exploring efficacy safety stenosis.
 Objective. To evaluate effectiveness long-term outcomes device Materials methods. We initiated a...
Introduction. Cardiovascular diseases have become the leading mortality cause, requiring a novel technologies and techniques for treatment. biomaterials play vital role as potentially eligible alternative modality an operation, covering gap between facilities patients in need of medical devices procedures.Purpose. The aim this essay is to explore analyze currently used cardiac surgery tools repair or replace damaged tissues. This study seeks obtain data which will help address research gaps...
The aim – to systematize information on key features of echocardiographic evaluation transcatheter aortic valve implantation (TAVI) procedure stages and their effectiveness in cardiac surgery, patients with severe stenosis.Materials methods. We initiated a single-center clinical study evaluate the XPand device initial analysis primary results was performed. Patients met inclusion criteria underwent full range examinations TAVI procedures using device. parameters for examination TAVI, which...
The use of xenotissue for the needs regenerative and cardiac medicine is a promising area tissue engineering.The decellularization process provides complete purification elastin-collagen matrix bovine pericardium from cells their components.The high concentrations sodium dodecyl sulfate glutaraldehyde can lead to damage architectonics.Therefore, purpose this study was microarchitectonics decellularized using low concentration (0.1% solution) avoiding glutaraldehyde.Further stabilization...