A5079298662- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Insect symbiosis and bacterial influences
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Irrigation Practices and Water Management
- Research on scale insects
Institut Agro Rennes-Angers
2019-2023
Institut de Recherche en Horticulture et Semences
2013-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2023
L'Institut Agro
2020-2023
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail
2019-2022
Université d'Angers
2019-2021
Abstract Of American origin, a wide diversity of Xylella fastidiosa strains belonging to different subspecies have been reported in Europe since 2013 and its discovery Italian olive groves. Strains from the multiplex (ST6 ST7) were first identified France 2015 urban natural areas. To trace back most probable scenario introduction France, molecular evolution rate this was estimated at 3.2165 × 10 -7 substitutions per site year, based on heterochronous genome sequences collected worldwide....
Xylella fastidiosa (Xf) is an insect-borne bacterium confined to the xylem vessels of plants. This plant pathogen has a broad host range estimated 560 species. Five subspecies with different but overlapping ranges have been described, only three are widely accepted, namely fastidiosa, multiplex, and pauca. Initially limited Americas, Xf detected in Europe since 2013. As management X. outbreaks depends on identification subspecies, accurate determination infected plants as early possible...
Xylella fastidiosa (
Xylella fastidiosa is a quarantine priority pest in Europe and several other countries as it represents major threat to many cultivated wild plants. Early detection of the pathogen needed reduce potential losses stop bacterial dissemination. Very few molecular methods are available for rapid field identify pathogen. Here, we evaluated new commercial test called AmplifyRP, based on recombinase polymerase amplification (RPA). The AmplifyRP was compared with quantitative PCR (qPCR) Harper's...
Identification of sequence types (ST) Xylella fastidiosa based on direct MultiLocus Sequence Typing (MLST) plant DNA samples is partly efficient. In order to improve the sensitivity X. identification, we developed a nested-MLST assay extracted DNA. This method was performed largely used scheme targeting seven housekeeping gene (HKG) loci (cysG, gltT, holC, leuA, malF, nuoL, petC). Samples analyzed included 49 species and two insect (Philaenus spumarius, Neophilaenus campestris) that were...
Abstract Different sequence types (ST) of Xylella fastidiosa were already identified in France and Spain based on direct MultiLocus Sequence Typing (MLST) plant DNA samples. However, typing is partly efficient. In order to improve the sensitivity X. identification, we developed a nested-MLST assay extracted DNA. This method was performed largely used scheme targeting seven housekeeping gene (HKG) loci ( cysG, gltT, holC, leuA, malF, nuoL, petC ). Nested primers designed from multi-sequence...
Abstract Of American origin, a wide diversity of Xylella fastidiosa strains belonging to different subspecies have been reported in Europe since 2013 and its discovery Italian olive groves. Strains from the multiplex (ST6 ST7) were first identified France 2015 urban natural areas. To trace back most probable scenario introduction France, molecular evolution rate this was estimated at 3.2165 × 10 -7 substitutions per site year, based on heterochronous genome sequences collected worldwide....
Abstract Xylella fastidiosa is an insect-borne bacterium confined to the xylem vessels of plants. This plant pathogen has a broad host range estimated 560 species. Five subspecies with different but overlapping ranges have been described, only three are widely accepted, namely , multiplex and pauca . Initially limited Americas, Xf detected in Europe since 2013. As management X. outbreaks depends on identification subspecies, accurate determination infected plants as early possible major...
Abstract Xanthomonas hortorum pv. carotae is a pathogen responsible for the bacterial blight of carrot. While it infects carrot fields worldwide, its diversity remains unknown. Here, we validated by PCR identification as X. most strains isolated from symptoms. We study their sequencing seven housekeeping genes. The analysis confirmed identity previously identified and highlighted presence two clusters inside pathovar . 18 non- have been other species. As clustered only in groups this...
Xanthomonas hortorum pv. carotae is a pathogen responsible for the bacterial blight of carrot. Although it infects carrot fields worldwide, its diversity remains unknown. Here, we validated by PCR identification as X. most strains isolated from symptomatic carrots. We studied their sequencing seven housekeeping genes. The analysis confirmed identity previously identified and highlighted presence two clusters inside pathovar carotae. 18 non- have been other species. As clustered only in...
Abstract Xylella fastidiosa ( Xf ) is a quarantine plant pathogen bacterium originating from the Americas and that has emerged in Europe 2013. can be detected directly on macerate using molecular methods such as real-time PCR, which sensitive technique. However, some plants may contain components act PCR reaction inhibitors, lead to false negative results or an underestimation of bacterial concentration present analyzed sample. Droplet digital (ddPCR) innovative tool based partitioning...
ABSTRACT Xylella fastidiosa ( Xf ) is a globally distributed plant pathogenic bacterium. The primary control strategy for diseases eradicating infected plants; therefore, timely and accurate detection necessary to prevent crop losses further pathogen dispersal. Conventional diagnostics primarily relies on quantitative PCR (qPCR) assays. However, these methods do not consider new or emerging variants due genetic recombination sensitivity limitations. We developed tested metagenomics pipeline...