A5038163975- Themes in Literature Analysis
- History of Science and Medicine
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Virginia Tech
2012-2024
American Headache Society
2019
Carolina Headache Institute
2019
William & Mary
1991-2015
AECC University College
2009
Saint Mary's College
2009
University of Southampton
2009
The University of Melbourne
1966
Stringent standards of water quality have prompted many horticultural enterprises to limit pollutant discharge associated with nutrient and pesticide applications. Collecting recycling effluent is a method that has been implemented by operations contain pollutants; however, plant pathogens may be spread through recycled effluent. In this study, Phytophthora Pythium spp. present in water-recycling irrigation system at perennial container nursery southwestern Virginia were characterized using...
Abstract Pathogen detection and identification are key elements in outbreak control of human, animal, plant diseases. Since many fungal pathogens cause similar symptoms, difficult to distinguish morphologically, grow slowly culture, culture-independent, sequence-based diagnostic methods desirable. Whole genome metagenomic sequencing has emerged as a promising technique because it can potentially detect any pathogen without culturing the need for pathogen-specific probes. However, efficient...
Recycled irrigation water is a primary source of inoculum for Phytophthora spp. and capable spreading propagules throughout nursery production. Chlorination commonly used by the industry to disinfest recycled water; however, chlorine has not been fully researched as disinfestant this purpose. In study, zoospores seven species eight isolates were exposed 2 min free available at 0.25, 0.5, 1.0, 2.0, 4.0 mg/liter. Zoospores, mycelial fragments, culture plugs P. nicotianae also concentrations...
Phytophthora diseases are commonly diagnosed on ornamental plants, but taxonomic identification to species can be intimidating. This illustrative guide is designed aid diagnosticians in morphological of certain spp. that have been reported irrigation and effluent water nurseries. Using both molecular methods, the authors identify diagnostic characteristics consistent easy use. Accepted for publication 6 April 2006. Published 21 June
Early disease detection is a prerequisite for enacting effective interventions control. Strains of the bacterial plant pathogen Xylella fastidiosa have recurrently spread to new crops in countries causing devastating outbreaks. So far, investigation outbreak strains and highly resolved phylogenetic reconstruction required whole-genome sequencing pure cultures, which are challenging obtain due fastidious nature X. . Here, we show that culture-independent metagenomic sequencing, using Oxford...
Ratios of certain organochlorine pollutants in fish tissues are significantly different between the eastern and western North Atlantic have been used to identify recent (<1 year) transatlantic migrants bluefin tuna (Thunnus thynnus). In this study we observed limited variation pollutant ratios across collections young-of-the-year (YOY) from (WNA) (ENA) years highly significant differences WNA ENA YOY samples each year for all combined. A increase was noted age-1 tuna, consistent with...
Xylella fastidiosa (
During the spring of 2007, approximately 2,000 switchgrass plants, representing 168 core germplasm, were established in a field nursery at Virginia Tech Kentland Farm Research Center Montgomery County, VA. These germplasms originally obtained from USDA Plant Genetic Resources Conservation Unit (Griffin, GA) and included both lowland upland ecotypes. After planting, plants allowed to establish for 3 years. In summers 2009, 2010, 2011, substantial infection foliar rust pathogen was observed on...
Global travel and trade in combination with climate change are expanding the geographic distribution of plant pathogens. The bacterium Xylella fastidiosa is a prime example. Native to Americas, it has spread Europe, Asia, Middle East. To assess risk that pathogen introductions pose crops newly invaded areas, key survey their diversity, host range, disease incidence relation climatic conditions where they already present. We performed X. grapevine Virginia using quantitative PCR, multilocus...
Trematode cercariae inhabit predictable environments and respond to trigger cues with genetically fixed releaser responses when foraging for the upstream host. The effect of light gravity on transmission Echinostoma caproni Biomphalaria glabrata was investigated experimentally. Transmission chambers were constructed clear polyvinyl chloride pipe. Snails constrained within chamber prevent movement, while permitting swim freely. A trial consisted 2 infected B. shedding E. placed at center...
In early May 2008 and 2009, peony samples (Paeonia spp.) with symptoms of leaf spot blight were submitted to the Virginia Tech Plant Disease Clinic. The was an unknown cultivar from a northern landscape. three cultivars (Dr. Alexander Fleming, Felix Crousse, Karl Rosenfield) in 2009 commercial nursery southwestern that reporting progressing severe blight, which rendered plants unsalable, on 75% 1,219 m2 block during 10-day period heavy rainfall. Bacterial streaming spots observed. On basis...
Plants in a single field of commercial tomato (Solanum lycopersicum) unidentified cultivars Virginia July, 2012, were observed showing stunting, leaf distortion, twisting and thickening, discoloration, color streaking ringspots on fruits. Serological tests negative for Cucumber mosaic virus, Groundnut ringspot Tomato spotted wilt chlorotic spot Impatiens necrotic Tobacco bushy stunt virus (Agdia, Inc., Elkhart, IN). Using membrane-based macroarray (3), hybridization was to 8 9 70-mer...
HomePlant DiseaseVol. 106, No. 6First Report of Laurel Wilt Disease Caused by Raffaelea lauricola on Sassafras in Virginia PreviousNext DISEASE NOTE OPENOpen Access licenseFirst VirginiaR. Gazis, K. M. DeWitt, L. Johnson, A. Chamberlin, H. Kennedy, Hansen, and E. BushR. Gazishttps://orcid.org/0000-0002-7979-176XUniversity Florida, Homestead, FL 33031, DeWittVirginia Department Forestry, Charlottesville, VA 22903, JohnsonVirginia ChamberlinVirginia KennedyNational Identification...
We developed a NitroPure Nitrocellulose (NPN) membrane-based method for sampling and storing grapevine sap virus detection. devised an efficient nucleic acid extraction the NPN membrane, resulting in 100% amplification success leafroll-associated 2 (GLRaV2) 3 (GLRaV3), rupestris stem pitting-associated (GRSPaV), A, B, red blotch (GRBV). This also allowed storage of recoverable 18 months at room temperature. created kit to survey GLRaV2, GLRaV3, GRBV Japanese vineyards. tested kits field 2018...
Reviewed by: Take Me Out to the Ball Game Elizabeth Bush Burke, Jim Game; written and illus. by Burke; lyrics Jack Norworth. Little, 200632p ISBN 0-316-75819-1$16.99 R Gr. 3-6 It will doubtless come as a surprise many young readers that song they've known since early youth actually has verses well chorus. Burke lays out Norworth in large print, casts song's protagonist, rabid fan Katie Casey, preteen with clean-cut, All-American beau, sends couple contested 1908 match-up between Giants Cubs....
Spring dead spot (SDS) of bermudagrass (Cynodon dactylon) is primarily caused by Ophiosphaerella herpotricha and korrae in North America. These two species respond differently to numerous management practices, grow optimally at different soil pH ranges, differ aggressiveness. Understanding the distribution regions where SDS occurs will allow turfgrass managers tailor their practices toward predominant present. A survey was conducted Mid-Atlantic United States which 1 14 samples expressing...
Reviewed by: Animal Talk: Mexican Folk Art Sounds in English and Spanish by Cynthia Weill Elizabeth Bush Weill, Spanish; with sculptures Rubi Fuentes Efrain Broa. Cinco Puntos, 2016 32p ISBN 978-1-941026-32-8 $14.95 R 1-4 yrs again collaborates traditional artists—in this title, Oaxacan wood carvers—in a presentation that does double duty as concept book art book. Complementary of an animal face each other across spread, on page narrator notes the animals “speak” differently Spanish: “What...