A5102008644- Congenital heart defects research
- Congenital Heart Disease Studies
- RNA modifications and cancer
- MicroRNA in disease regulation
- Developmental Biology and Gene Regulation
- Zebrafish Biomedical Research Applications
- Genetics and Neurodevelopmental Disorders
- Coronary Artery Anomalies
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Mitochondrial Function and Pathology
- Genomics and Chromatin Dynamics
- Tissue Engineering and Regenerative Medicine
- Genomic variations and chromosomal abnormalities
- Wnt/β-catenin signaling in development and cancer
- Protease and Inhibitor Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Genomics and Phylogenetic Studies
- Smart Grid Security and Resilience
- Cleft Lip and Palate Research
- CRISPR and Genetic Engineering
- Bone and Dental Protein Studies
Children's Hospital of Fudan University
2014-2024
Oak Ridge National Laboratory
2024
Obstetrics and Gynecology Hospital of Fudan University
2022
Fudan University Shanghai Cancer Center
2022
State Key Laboratory of Genetic Engineering
2017
Nanjing Tech University
2016
Shanghai Medical College of Fudan University
2007-2013
Fudan University
2013
Children's Hospital of Philadelphia
1995-1997
University of Pennsylvania
1995-1996
Abstract Dysregulated maternal nutrition, such as vitamin deficiencies and excessive levels of glucose fatty acids, increases the risk for congenital heart disease (CHD) in offspring. However, association between amino‐acid CHD is unclear. Here, it shown that increased leucine plasma during first trimester are associated with elevated High increase embryonic lysine‐leucylation (K‐Leu), which catalyzed by leucyl‐tRNA synthetase (LARS). LARS preferentially binds to catalyzes K‐Leu modification...
Short-chain enoyl-CoA hydratase 1 (ECHS1) deficiency plays a role in cardiomyopathy. Whether ECHS1 causes or is only associated with cardiomyopathy remains unclear. By using Echs1 heterogeneous knockout (Echs1+/-) mice, we found that caused cardiac dysfunction, as evidenced by diffuse myocardial fibrosis and upregulated fibrosis-related genes. Mechanistically, interacts the p300 nuclear localization sequence, preventing its translocation fibroblasts. promotes translocation, leading to...
Abstract Objectives microRNA‐29 (miR‐29) family have shown different expression patterns in cardiovascular diseases. Our study aims to explore the effect and mechanism of miR‐29 on cardiac development. Materials methods A total 13 patients with congenital heart disease (CHD) 7 controls were included our study. Tissues obtained from right ventricular outflow tract (RVOT) after surgical resection or autopsy. The next‐generation sequencing was applied screen microRNA profiles CHD. Quantitative...
Abstract Considerable remodeling of the extracellular matrix as well cellular migration takes place during embryogenesis. Since metalloproteinase MMP‐9 is implicated in these functions cancer cells, we studied patterns expression mRNA development post‐implantation mouse embryos. was detected using ribonuclease protection assay poly A + RNA from 13 to 17 day embryos, but not at 11 days. In order localize transcripts, situ hybridization performed on sections murine embryos 7.5 15 days...
Abstract TBX5 is a vital transcription factor involved in cardiac development dosage-dependent manner. But little known about the potential association of 3′ untranslated region (UTR) variations with congenital malformations. This study aimed to investigate relationship between 3′UTR variants and risk for heart disease (CHD) susceptibility two Han Chinese populations, reveal its molecular mechanism. The CHD was examined 1 177 patients 990 healthy controls independent case–control studies....
Both retinoic acid (RA) and Tbx1 are definitively indispensable for the development of pharyngeal arches. The defects produced by a loss highly resemble those induced hyper- hypo-RA. Based on these similarities, effects RA expression pattern were explored during arch in zebrafish. Whole-mount situ hybridization real-time quantitative PCR used. Zebrafish embryos treated with 5 x 10(-8)mol/L 10(-7)mol/L at 12.5 hours post fertilization 1.5 hours, respectively. showed that was expressed cardiac...
Ventricular septal defects (VSDs) constitute the most prevalent congenital heart disease (CHD), occurs either in isolation (isolated VSD) or combination with other cardiac (complex VSD). Copy number variation (CNV) has been highlighted as a possible contributing factor to etiology of many diseases. However, little is known concerning involvement CNVs isolated complex VSDs. We analyzed 154 unrelated Chinese individuals VSD by chromosomal microarray analysis. The subjects were recruited from...
Abstract Maternal hyperglycemia potentially inhibits the development of fetal heart by suppressing cardiomyocyte proliferation and promoting apoptosis. Different studies have indicated that miRNAs are key regulators proliferation, differentiation, apoptosis play a protective role in variety cardiovascular diseases. However, biological function miRNA-23a hyperglycemia-related injury is not fully understood. The present study investigated effect miRNA-23a-3p on cell myocardial model induced...
Abstract BACKGROUND: Maternal diabetes increases the risk of congenital heart defects in infants, and hyperglycemia acts as a major teratogen. Multiple steps cardiac development, including endocardial cushion morphogenesis development neural crest cells, are challenged under elevated glucose conditions. However, direct effect on embryo organogenesis remains to be investigated. METHODS: Zebrafish embryos different stages were exposed D‐glucose for 12 or 24 hr determine sensitive window during...
The hippo (Hpo) signaling pathway plays a critical role in regulation of organ size.The kinase cascade ultimately antagonizes the transcriptional co-activator Yki/YAP, which is key regulator cell proliferation and apoptosis.In this study, we performed knocking down study using antisense morpholino (MO) reagents found that zebrafish YAP, Hpo pathway, early embryonic development.At cellular level, yap inhibition increases apoptosis decreases proliferation.Reduction function severely delays...
Congenital heart disease (CHD) is one of the most common birth defects in humans, but its genetic etiology remains largely unknown despite decades research. The Notch signaling pathway plays critical roles embryonic cardiogenesis. Mind bomb 1 (Mib1) a vital protein that activates through promoting ubiquitination, endocytosis and subsequent activation ligands. Previous studies show Mib1 knockout mice completely abolishes signaling, leading to cardiac deformity. However, function MIB1...
Cardiac development is a peculiar process involving coordinated cellular differentiation, migration, proliferation, and apoptosis. DNA methylation plays key role in genomic stability, tissue-specific gene expression, cell Hypomethylation the global genome has been reported cardiovascular diseases. However, little known about impact specific mechanism of hypomethylation on cardiomyocytes. In present study, we explored methyltransferase inhibitors 5-azacytidine cardiac development. vivo...
Methotrexate (MTX), an antagonist of folic acid, can inhibit dihydrofolate reductase (DHFR) which is great importance in the synthesis tetrahydrofolic acid and embryonic development. In this study, we found that after being exposed to 1.5 mM MTX at 6-10 hours post-fertilization, zebrafish embryos fail form normal cardiovascular system. MTX-treated embryos, morphological development ventricle atrium was disrupted, cardiac twist abnormal, heart rate ventricular shortening fraction were...
As a member of the miR-29 family, miR-29b regulates global DNA methylation through target methyltransferases (DNMTs) and acts as both key effector in methylation. In this study, we found that miR-29b-3p expression was inversely correlated with DNMT heart tissues patients congenital disease (CHD), but whether it interacts DNMTs cardiomyocytes remains unknown. Further results revealed feedback loop between cardiomyocytes. Moreover, inhibitor relieved deformity hypomethylated zebrafish restored...
Folic acid is very important for embryonic development and folic inhibition can cause congenital heart defects in vertebrates. Dihydrofolate reductase (DHFR) a key enzyme folate-mediated metabolism. The dysfunction of DHFR disrupts the biological processes which participates in. gene conserved during vertebrate evolution. It to investigate roles cardiac developments. In this study, we showed that knockdown resulted abnormal developments zebrafish embryos early stages. Obvious malformations...
Abstract Pericardial cavity formation and epithelialization of the cardiac precursor cell population constitute a critical developmental period that precedes stable commitment differentiation. These events delineate myocardial endocardial in embryo. Restriction Na/K‐ATPase (the sodium pump) expression to pre‐cardiomyocyte lateral membranes coincides with these events. has been implicated developmentally cavitation maintaining membrane potential. Experiments were undertaken determine if...
As one of the major epigenetic modifications, DNA methylation is constantly regulated during embryonic development, cell lineage commitment, and pathological processes. To facilitate real-time observation methylation, we generated a transgenic zebrafish reporter (zebraRDM) via knockin an mCherry-fused methyl-CpG binding domain (MBD) probe driven by bactin2 promoter. The colocalized with heterochromatin, its intensity was positively correlated 5 mC immunostaining at subcellular resolution in...