A5000215774- Colorectal Cancer Surgical Treatments
- Colorectal Cancer Screening and Detection
- Cell Adhesion Molecules Research
- Colorectal and Anal Carcinomas
- Genetic factors in colorectal cancer
- Colorectal Cancer Treatments and Studies
- Gastric Cancer Management and Outcomes
- Neuroendocrine regulation and behavior
- Radiomics and Machine Learning in Medical Imaging
- Cancer Immunotherapy and Biomarkers
- Drug Transport and Resistance Mechanisms
- Cancer Research and Treatments
- Peptidase Inhibition and Analysis
- Cytomegalovirus and herpesvirus research
- Melanoma and MAPK Pathways
- Mitochondrial Function and Pathology
- Ion Transport and Channel Regulation
- Electrolyte and hormonal disorders
- Clinical practice guidelines implementation
- Cancer, Stress, Anesthesia, and Immune Response
- Renal cell carcinoma treatment
- Lymphoma Diagnosis and Treatment
- Cancer Genomics and Diagnostics
- Estrogen and related hormone effects
- Neuroscience of respiration and sleep
University of South Florida
2025
Memorial Sloan Kettering Cancer Center
2020-2024
University of California, Irvine
2007-2023
Kettering University
2022
National Institutes of Health
2021
American Marketing Association
2021
Rutgers New Jersey Medical School
2019
Rutgers, The State University of New Jersey
2019
Chungnam National University
2016
University of Rochester Medical Center
2002
PURPOSE Prospective data on the efficacy of a watch-and-wait strategy to achieve organ preservation in patients with locally advanced rectal cancer treated total neoadjuvant therapy are limited. METHODS In this prospective, randomized phase II trial, we assessed outcomes 324 stage or III adenocarcinoma induction chemotherapy followed by chemoradiotherapy (INCT-CRT) consolidation (CRT-CNCT) and either mesorectal excision (TME) basis tumor response. Patients both groups received 4 months...
Multiple steroid receptors (SR) have been proposed to localize the plasma membrane. Some structural elements for membrane translocation of estrogen receptor alpha (ER alpha) described, but mechanisms relevant other are entirely unknown. Here, we identify a highly conserved 9 amino acid motif in ligand binding domains (E domains) human/mouse ER and beta, progesterone A B, androgen receptor. Mutation phenylalanine or tyrosine at position-2, cysteine position 0, hydrophobic isoleucine/leucine...
4008 Background: Organ preservation (OP) with a watch and wait strategy (WW) total neoadjuvant therapy (TNT) are new treatment paradigms for patients locally advanced rectal cancer. The safety efficacy of WW TNT have not been studied prospectively. Methods: Patients MRI stage II III adenocarcinoma were randomized to 4 months FOLFOX or CAPEOX before (Induction) after (Consolidation) fluorouracil capecitabine based chemoradiotherapy (CRT). re-staged 8-12 weeks finishing digital exam, flexible...
JCO
Importance Assessing clinical tumor response following completion of total neoadjuvant therapy (TNT) in patients with locally advanced rectal cancer is paramount to select for watch-and-wait treatment. Objective To assess organ preservation (OP) and oncologic outcomes according grade. Design, Setting, Participants This was secondary analysis the Organ Preservation Patients Rectal Adenocarcinoma trial, a phase 2, nonblinded, multicenter, randomized trial. Randomization occurred between April...
Estrogen receptors (ERs) α and β exist as nuclear, cytoplasmic, membrane cellular pools in a wide variety of organs. The relative contributions each ERα pool to vivo phenotypes resulting from estrogen signaling have not been determined. To address this, we generated transgenic mouse expressing only functional E domain at the plasma (MOER). Cells isolated many organs showed localized no other receptor pools. Liver cells MOER wild type mice responded 17-β-estradiol (E2) with comparable...
Abstract Background Cellular metabolism is an integral component of cellular adaptation to stress, playing a pivotal role in the resistance cancer cells various treatment modalities, including radiotherapy. In response radiotherapy, engage antioxidant and DNA repair mechanisms which mitigate remove damage, facilitating cell survival. Given reliance these on amino acid metabolism, we hypothesised that controlling exogenous availability non-essential acids serine glycine would radiosensitise...
Abstract Background Total neoadjuvant therapy (TNT) improves tumor response in locally advanced rectal cancer (LARC) patients compared to chemoradiotherapy alone. The effect of TNT on patient survival has not been fully investigated. Materials and Methods This was a retrospective case series with LARC at comprehensive center. Three hundred eleven received (chemoRT) as the sole treatment planned adjuvant chemotherapy, 313 (induction fluorouracil oxaliplatin-based chemotherapy followed by...
Background MRI plays a crucial role in restaging locally advanced rectal cancer treated with total neoadjuvant therapy (TNT); however, prospective studies have not evaluated its ability to accurately select patients for nonoperative management. Purpose To evaluate the of predict oncologic outcomes and identify imaging features associated residual disease (RD) after TNT. Materials Methods This was secondary analysis Organ Preservation Rectal Adenocarcinoma (OPRA) trial, which randomized...
To characterize the relationship between tumor-infiltrating lymphocytes (TIL), tumor mutational burden (TMB), and genetic alterations in microsatellite stable (MSS), instability (MSI), or mutant POLE/POLD1 colon cancer.Four hundred ninety-nine resected stage I-III tumors treated 2014 2019 were assessed for TIL; somatic mutations, copy number alterations, structural changes > 400 oncogenes; MSI status.Of 499 analyzed, 313 MSS, 175 MSI, 11 had pathogenic mutations. Both percentage of with a...
Recombination promotes retrovirus evolution. It involves transferring a growing DNA primer from one genomic RNA template in the virus to other. Strand transfer results vitro suggested that pausing of reverse transcriptase during synthesis allows enhanced RNase H cleavage initial, or donor, facilitates interaction with acceptor template. Hairpins are common structures RNAs induce pausing. Analyzing transfers hairpins by base substitution markers showed sites well beyond site We developed...
Abstract Background Prospective randomized trials have not yet identified baseline features predictive of organ preservation in locally advanced rectal cancers treated with total neoadjuvant therapy and a selective watch-and-wait strategy. Methods This was secondary analysis the OPRA trial, which patients stage II–III adenocarcinoma to receive either induction or consolidation therapy. Patients were recommended for mesorectal excision, watch wait based on clinical response at 8 ± 4 weeks...
Enzymes of hormone-dependent cAMP metabolism and other enzymes were studied in renal medulla, cortex, liver hypothyroid rats treated for 1 week with T4. In the group, basal activity medullary adenylate cyclase stimulated by vasopressin markedly decreased. Treatment T4 resulted an increase a decrease phosphodiesterase. studies enzymes, only cytochrome c oxidase was decreased hypothyroidism, but it not influenced treatment. vitro, addition 10-7-10-5 M had no effect on or its stimulation NaF....
A barrier to the widespread adoption of watch-and-wait management for locally advanced rectal cancer is inaccuracy and variability identifying tumor response endoscopically in patients who have completed total neoadjuvant therapy (chemoradiotherapy systemic chemotherapy).
Abstract KRAS mutation in colorectal cancer is associated with aggressive tumor behavior through increased invasiveness and higher rates of lung metastases, but the biological mechanisms behind these features are not fully understood. In this study, we show that KRAS-mutant upregulates integrin α6β4 ERK/MEK signaling. Knocking-out β4 (ITGB4) specifically depleted expression resulted a reduction invasion migration ability cells. We also observed number area metastatic foci mice were injected...
Human immunodeficiency virus reverse transcribes its single-stranded RNA genome making a DNA copy. As synthesis proceeds, the is simultaneously degraded to oligomers; one of these, polypurine tract, primes plus strand DNA. The viral transcriptase (RT) degrades all non-polypurine tract oligomers. We show that unlike other polymerases retroviral RT can bind either end an annealed primer, 5′-end for degradation and 3′-end synthesis. competition between two binding modes at any primer determines...
Colon tumors with deficient DNA mismatch repair (dMMR) are generally infiltrated by T cells more densely than proficient (pMMR). However, high numbers of tumor-infiltrating lymphocytes (TILs) found in select pMMR tumors, and low TILs seen dMMR tumors. In this study, we compared T-cell repertoires 20 27 colon TIL counts. We that clonal their repertoire is less rich the group, TIL-high were was TIL-low but diversity comparable to These findings suggest clonally expand possibly response MMR...
Somatic mutations in the KRAS oncogene are associated with poor outcomes locally advanced rectal cancer but underlying biologic mechanisms not fully understood. We profiled mRNA 76 adenocarcinomas from patients that were enrolled a prospective clinical trial and investigated differences gene expression between mutant (KRAS-mt) KRAS-wild-type (KRAS-wt) patients. found KRAS-mt tumors display lower of genes related to tumor stroma remodeling extracellular matrix. validated our findings using...
<h3>Importance</h3> Accurate clinical staging is important in rectal cancer because it determines the appropriate treatment and prognosis. Despite use of multiple diagnostic imaging tools, sometimes difficult to clinically distinguish stage I tumors from II or III locally advanced disease. Identification differentiating microRNAs (miRNAs) between these 2 groups may improve power provide insight into biology tumor progression. <h3>Objectives</h3> To investigate differences expression miRNAs...