A5083835142- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
- Folate and B Vitamins Research
- Birth, Development, and Health
- Gestational Diabetes Research and Management
- Circular RNAs in diseases
- Renal and related cancers
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Maternal and fetal healthcare
- Glioma Diagnosis and Treatment
- Mitochondrial Function and Pathology
- Pregnancy-related medical research
- Asthma and respiratory diseases
- Pulmonary Hypertension Research and Treatments
- Pluripotent Stem Cells Research
- Cardiovascular Issues in Pregnancy
- Biomedical Text Mining and Ontologies
- Preterm Birth and Chorioamnionitis
- Maternal and Perinatal Health Interventions
- RNA modifications and cancer
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Molecular Biology Techniques and Applications
- COVID-19 Impact on Reproduction
Flinders University
2019-2025
Flinders Medical Centre
2021-2023
Adelaide Institute for Sleep Health
2022
Hunter Medical Research Institute
2017-2019
University of Newcastle Australia
2018-2019
Chronic obstructive pulmonary disease (COPD) is the third leading cause of morbidity and death imposes major socioeconomic burdens globally. It a progressive disabling condition that severely impairs breathing lung function. There lack effective treatments for COPD, which direct consequence poor understanding underlying mechanisms involved in driving pathogenesis disease. Toll-like receptor (TLR)2 TLR4 are implicated chronic respiratory diseases, including asthma fibrosis. However, their...
Are any microRNAs (miRNAs) that target the placental renin-angiotensin system (RAS) in human placenta suppressed early gestation?Overall, 21 miRNAs with predicted RAS mRNA targets were less abundant versus term placentae and nine more highly expressed.Regulation of expression could alter development therefore normal pregnancy outcome. The genes encoding prorenin (REN), angiotensinogen, (pro)renin receptor, angiotensin converting enzyme 2, II type 1 receptor are highest gestation, at a time...
ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups from...
In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in development. Among other functions, signalling through angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting RAS mRNAs are downregulated at this time. We propose that gestation miRNAs target downregulated, allowing for increased expression proliferation required adequate placentation. HTR-8/SVneo cells (an immortalized trophoblast cell line)...
Folic acid (FA) food fortification in Australia has resulted a higher-than-expected intake of FA during pregnancy. High is associated with increased insulin resistance and gestational diabetes. We aimed to establish whether maternal one-carbon metabolism hormones that regulate glucose homeostasis change healthy pregnancies post-FA fortification. Circulating folate, B12, homocysteine, prolactin (PRL), human placental lactogen (hPL) growth hormone (GH2) were measured early pregnancy blood...
In contrast to most non-malignant tissue, cells comprising the brain tumour glioblastoma (GBM) preferentially utilise glycolysis for metabolism via “the Warburg effect”. Research into therapeutics targeting disease’s highly glycolytic state offer a promising avenue improve patient survival. These studies often employ GBM cell lines in vitro which translate poorly vivo context. The metabolic traits of five used were assessed and compared primary matched, healthy tissue. patient-derived lines,...
Parturition signals the end of immune tolerance in pregnancy. Term labour is usually a sterile inflammatory process triggered by damage associated molecular patterns (DAMPs) as consequence functional progesterone withdrawal. Activation DAMPs recruits leukocytes and cytokine responses myometrium, decidua, cervix fetal membranes. Emerging evidence shows components inflammasome are detectable both maternal decidua placenta. However, activation placental with respect to mode delivery has not...
High yield and integrity of placental RNA are crucial for transcriptomics studies. We assessed the effects time to collection post-delivery; tissue storage, amount method used extraction; mode delivery; type on total yield. The optimal protocol extraction from includes cryofreezing sample upon 50 mg using TRIzol reagent. Decidua yielded highest quantity/mg tissue, followed by villous chorion. Comparisons with murine kidney HEK293T show lower yield, likely due highly dense heterogeneous...
Human placental renin-angiotensin system (RAS) expression is highest in early gestation, at a time when oxygen tension its lowest (1-3%), and promotes development. Some miRNAs predicted to target RAS mRNAs are downregulated gestation. We tested the hypothesis that low suppresses of mRNAs, thus increasing concentrations mRNAs. HTR-8/SVneo cells were cultured 1, 5 20% for 48 h. Differences miRNA measured on an Affymetrix microarray (n = 3/group). Those or decreased confirmed by qPCR 9/group)....
The search for novel microRNA (miRNA) biomarkers in plasma is hampered by haemolysis, the lysis and subsequent release of red blood cell contents, including miRNAs, into surrounding fluid. biomarker potential miRNAs comes part from their multicompartment origin long-lived nature miRNA transcripts plasma, giving researchers a functional window tissues that are otherwise difficult or disadvantageous to sample. inclusion red-blood-cell-derived downstream analysis introduces source error...
<title>Abstract</title> Background In contrast to most non-malignant cells, cells comprising Glioblastoma multiforme (GBM), a deadly brain tumour with extremely poor prognosis, preferentially utilise glycolysis over oxidative phosphorylation for metabolism in phenomenon known as the ‘Warburg effect’. As effective treatments GBM are severely lacking, research into therapeutics targeting disease’s highly glycolytic state offer promising avenue improve patient survival. These studies often...
In a paper published in EClinicalMedicine, Eran Bornstein and colleagues [[1]Bornstein E Eliner Y Chervenak FA Grünebaum A. Concerning trends maternal risk factors the United States: 1989-2018.EClinicalMedicine. 2020; https://doi.org/10.1016/j.eclinm.2020.100657Summary Full Text PDF PubMed Scopus (3) Google Scholar] report data on pregnancy complications that increase morbidity mortality over 120 million American women with livebirths from 1989–2018. They show significant increases time of...
Abstract ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups...
Abstract Unexplained stillbirth may occur due to premature placental ageing, with unexpected deterioration of function for gestational age. Circular RNAs (circRNAs) are enzyme-resistant RNA molecules that accumulate in ageing tissues. Furthermore, circRNAs bind gDNA directly, forming circRNA:DNA complexes which can induce DNA breaks. This study investigated circRNA accumulation age healthy and placentae determined whether directly interact causing damage. Placenta samples (n=60 term...