A5073801196- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Renal and related cancers
- Retinal Development and Disorders
- Advanced biosensing and bioanalysis techniques
- Pluripotent Stem Cells Research
- RNA regulation and disease
- RNA Interference and Gene Delivery
The University of Adelaide
2021-2025
South Australian Health and Medical Research Institute
2021-2025
Precise genomic modification using prime editing (PE) holds enormous potential for research and clinical applications. In this study, we generated all-in-one (PEA1) constructs that carry all the components required PE, along with a selection marker. We tested these (with selection) in HEK293T, K562, HeLa mouse embryonic stem (ES) cells. discovered PE efficiency HEK293T cells was much higher than previously observed, reaching up to 95% (mean 67%). The K562 cells, however, remained low. To...
ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups from...
Abstract Ensuring sufficient gRNA transcript levels is critical for obtaining optimal CRISPR-Cas9 gene editing efficiency. The standard scaffold contains a sequence of four thymine nucleotides (4T), which known to inhibit transcription from Pol III promoters such as the U6 promoter. Our study showed that using plasmid transfection protocols, presence these 4Ts did not significantly affect efficiency, most gRNAs tested (55 gRNAs) achieved near-perfect outcomes. We observed with lower activity...
Abstract ACE2 expression is altered in pregnancy disorders and gene variants are associated with several major complications including small-for-gestational-age, fetal growth restriction preeclampsia. This study utilised gene-editing to generate both knockout rs2074192 placental organoids, facilitating mechanistic studies into the role of development, effect carriage CC, CT TT genotypes. Parameters cell organoid were measured, together qPCR, Western Blotting, ELISA assessments, all groups...
ABSTRACT Precise genomic modification using prime editing (PE) holds enormous potential for research and clinical applications. Currently, the delivery of PE components to mammalian cell lines requires multiple plasmid vectors. To overcome this limitation, we generated all-in-one (PEA1) constructs that carry all required PE, along with a selection marker. We tested these (with selection) in HEK293T, K562, HeLa mouse embryonic stem (ES) cells. discovered efficiency HEK293T cells was much...