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Ryan H. B. Lee

ORCID: 0009-0003-2050-4847
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About
Contact & Profiles
A5012518044
Research Areas
  • CRISPR and Genetic Engineering
  • Plant Molecular Biology Research
  • Virus-based gene therapy research
  • Advanced biosensing and bioanalysis techniques
  • Plant Gene Expression Analysis
  • Photosynthetic Processes and Mechanisms
  • RNA Interference and Gene Delivery
  • RNA regulation and disease

The University of Adelaide
 2025

South Australian Health and Medical Research Institute
 2025

 Optimal SpCas9- and SaCas9-mediated gene editing by enhancing gRNA transcript levels through scaffold poly-T tract reduction
OPENALEX - Publications 
Yu Chinn Joshua Chey Luke Gierus Caleb Lushington Jayshen Arudkumar Ashleigh Geiger and 8 more Lachlan G. Staker Louise J. Robertson Chandran Pfitzner Jesse G. Kennedy Ryan H. B. Lee G.I. Godahewa Fatwa Adikusuma Paul Q. Thomas

10.1186/s12864-025-11317-2 article EN cc-by-nc-nd BMC Genomics 2025-02-12
 Enhancing gRNA Transcript levels by Reducing the Scaffold Poly-T Tract for Optimal SpCas9- and SaCas9-mediated Gene Editing
OPENALEX - Publications 
Yu Chinn Joshua Chey Luke Gierus Caleb Lushington Jayshen Arudkumar Ashleigh Geiger and 8 more Lachlan G. Staker Louise J. Robertson Chandran Pfitzner Jesse G. Kennedy Ryan H. B. Lee G.I. Godahewa Paul Q. Thomas Fatwa Adikusuma

Abstract Ensuring sufficient gRNA transcript levels is critical for obtaining optimal CRISPR-Cas9 gene editing efficiency. The standard scaffold contains a sequence of four thymine nucleotides (4T), which known to inhibit transcription from Pol III promoters such as the U6 promoter. Our study showed that using plasmid transfection protocols, presence these 4Ts did not significantly affect efficiency, most gRNAs tested (55 gRNAs) achieved near-perfect outcomes. We observed with lower activity...

10.1101/2024.07.19.604224 preprint EN bioRxiv (Cold Spring Harbor Laboratory) 2024-07-19
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