A5004748290- Microtubule and mitosis dynamics
- Immune Cell Function and Interaction
- Image Processing Techniques and Applications
- Immunotherapy and Immune Responses
- Protist diversity and phylogeny
- T-cell and B-cell Immunology
- Cell Image Analysis Techniques
- DNA Repair Mechanisms
- Gene Regulatory Network Analysis
- 14-3-3 protein interactions
- Cancer Genomics and Diagnostics
- Reproductive System and Pregnancy
- Photosynthetic Processes and Mechanisms
- Micro and Nano Robotics
- Fungal and yeast genetics research
- Advanced Vision and Imaging
- Cell death mechanisms and regulation
- Single-cell and spatial transcriptomics
- Chromosomal and Genetic Variations
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- IL-33, ST2, and ILC Pathways
- Protein Structure and Dynamics
- Online Learning and Analytics
IFOM
2017-2025
Bocconi University
2018
T follicular helper (Tfh) cells are fundamental for B cell selection and antibody maturation in germinal centers. Circulating Tfh (cTfh) constitute a minor proportion of the CD4+ peripheral blood, but their clonotypic relationship to populations resident lymph nodes extent which they differ from non-Tfh have been unclear. Using donor-matched blood tonsil samples, we investigate receptor (TCR) sharing between tonsillar populations. TCR transcript sequencing reveals considerable clonal overlap...
Microtubules are polymers required for chromosome segregation. Their drug-induced hyperstabilization impairs segregation and is an established anti-cancer therapy. How cells respond to microtubule hyperstabilization, however, incompletely understood. To study this, we evolved budding yeast expressing a microtubule-hyperstabilizing tubulin mutant isolated adapted strains. Aneuploidy of specific chromosomes carrying the regulators STU2 VIK1/KAR3 was first observable adaptation. In longer run,...
Abstract Accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing-based object methods. In recent years, many applications have attempted to integrate deep-learning frameworks this task, but most still heavily rely on consecutive frame-based or other premises that hinder generalized learning. To address issue, we aimed develop novel deep-learning-based method assumes can be tracked by their spatio-temporal...
Drugs that impair microtubule dynamics alter microtubule-kinetochore attachment and invoke the mitotic checkpoint which arrests cells in mitosis. The arrest can last for hours, but it is leaky: adapt (i.e., slip out of it) exit from Here, we investigate mechanism allows to escape, whether possible prevent it. Based on a model includes presence positive feedback loop, escape described as stochastic transition driven by fluctuations molecular components ON OFF state. According model, drug...
The mitotic checkpoint (also called spindle assembly checkpoint) is a signaling pathway that ensures faithful chromosome segregation. Mitotic proteins inhibit the anaphase-promoting complex (APC/C) and its activator Cdc20 to prevent precocious anaphase. Checkpoint leads of APC/C, Cdc20, proteins, in which APC/C inactive. In principle, this final product can be obtained via different pathways, whose relevance still needs fully ascertained experimentally. Here, we use mathematical models...
Drugs targeting microtubules rely on the mitotic checkpoint to arrest cell proliferation. The prolonged induced by such drugs is followed a G1 arrest. Here, we follow for several weeks fate of G1-arrested human cells after treatment with nocodazole. We find that small fraction escapes from and resumes These escaping experience reduced DNA damage p21 activation. Cells surviving are enriched anti-apoptotic proteins, including Triap1. Increasing Triap1 levels allows survive first lower p21;...
ABSTRACT T follicular helper (Tfh) cells are fundamental for B cell selection and antibody maturation in germinal centers. Circulating Tfh (cTfh) constitute a minor proportion of the CD4 + peripheral blood, but their clonotypic relationship to populations resident lymph nodes extent which they differ from non-Tfh has been unclear. Using donor-matched blood tonsil samples we investigated receptor (TCR) sharing between tonsillar populations. TCR transcript sequencing revealed considerable...
ABSTRACT Microtubules, polymers of alpha- and beta-tubulin, are essential cellular components. When microtubule polymerization is hindered, cells delayed in mitosis, but eventually they manage to proliferate with massive chromosome missegregation. Several studies have analyzed the first cell division upon microtubules impairing conditions. Here, we asked how cope on long term. Taking advantage mutations evolved lab for ∼150 generations 24 populations yeast unable properly polymerize...
The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object methods. In recent years, several existing and new applications have attempted to integrate deep-learning frameworks this task, but most them still heavily rely on consecutive frame embedded in their architecture or other premises that hinder generalized learning. To address issue, we aimed develop method relies solely the assumption can...